LOCUS       sequence-36371-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Schnutgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, 
            Devries I, Wurst W, Hyman A, Mann M, von Melchner H
  TITLE     Resources for proteomics in mouse embryonic stem cells.
  JOURNAL   Nat Methods. 2011 Feb . 8(2):103-4.
  PUBMED    21278719
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Feb . 8(2):103-4."
COMMENT     Sequence Label: pDTnLAPP2A frame 2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             147..203
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(510..529)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
       61 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
      121 ctcgtccgag ggcaaaggaa cgcggagcca cgaacttctc tctgttaaag caagcaggag
      181 atgttgaaga aaaccccggg cccatggtgt ccaagggcga ggaactgttc accggcgtgg
      241 tgcccatcct ggtggagctg gacggcgacg tgaacggcca caagttcagc gtgtccggcg
      301 agggcgaagg cgacgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      361 agctgcccgt gccctggccc accctggtga ccaccctgac ctacggcgtg cagtgcttca
      421 gcagataccc cgaccacatg aagcagcacg atttcttcaa gagcgccatg cccgagggct
      481 acgtccagga gcgcaccatc tttttcaagg acgacggcaa ctacaagacc agggccgagg
      541 tgaagttcga gggcgacaca ctggtgaaca ggatcgagct gaagggcatc gacttcaaag
      601 aggacggcaa catcctgggc cacaagctgg aatacaacta caacagccac aacgtgtaca
      661 ttatggccga caagcagaag aacggcatca aggtcaactt caagatccgg cacaacatcg
      721 aggacggcag cgtgcagctg gccgaccact accagcagaa cacccccatc ggcgacggcc
      781 ccgtgctgct gcccgacaac cactacctga gcacccagag cgctctcagc aaggacccca
      841 acgagaagcg ggaccacatg gtgctgaaag aatttgtgac agccgccgga atcaccctgg
      901 gcatggacga gctgtacaag agcagcggcc cccagcggca gcagcagc
//
