LOCUS       sequence-36372-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP TEV LIC cloning vector (2M-T)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..111)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(127..150)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gttagcagcc ggatcagcgc gcacgcgtgg taccggccgg ccaccaggtg gcgcgccgcg
       61 atcgcggatc cgttatccac ttccaatatt ggattggaag tacaggtttt cctcgatccc
      121 attagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg cggcgttgat
      181 caccgcagta cgcacggcat accagaaagc ggacatctgc gggatgttcg gcatgatttc
      241 acctttctgg gcgttttcca tagtggcggc aatacgtgga tctttcgcca actcttcctc
      301 gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt ccagaccttc
      361 atcagtcagc agatagtttt cgaggaactc ttttgccagc tctttgttcg gactggcggc
      421 gttaatacct gcgctcagca cgccaacgaa cggtttggat ggttgaccct tgaaggtcgg
      481 cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg cccacgggcc
      541 gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg agtaatcggt
      601 gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac ccgctttcgc
      661 gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat acttgaacgc
      721 ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca ggttgaacat
      781 cagcgcgctc ttacctttcg ctttcagttc tttatccagc gccgggatct cttccc
//
