LOCUS       sequence-363733-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of YAP1 S94A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D,
            White EA
  TITLE     YAP1 activation by human papillomavirus E7 promotes basal cell 
            identity in squamous epithelia.
  JOURNAL   Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466.
  PUBMED    35170430
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-16"; volume: "11. pii: 75466. doi"; pages: " 
            10.7554/eLife.75466"
COMMENT     Sequence Label: p8328 pLIX YAP1 S94A
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    60..78
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    96..114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    131..149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    203..221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    238..256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    274..292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    354..471
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(684..728)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    913..1146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1639..1764
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1811..1991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1992..2218)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2246..2266)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2246..2264)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2285..2301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2285..2301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2298..2320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2309..2325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2333..2339,2340..2357,2358..2363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2378..2399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2516..2533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2687..3275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2767..2786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3446..4237,4238..4306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4069..4088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4307..4378)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4378..4513
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4553..4674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4564..4583)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4618..4637
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4746..4979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5184..5195
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5593..5613
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5653..5661,5662..5697,5698..5700,5701..5736,
                     5737..5739,5740..5775,5776..5781,5782..6399))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6400..6453)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6454..7050)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6534..6554)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7031..7050
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7060..7570)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7123..7141)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7580..7597)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7613..7639)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    7647..7671
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9138..9162)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(join(9191..9205,1..300))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(join(9194..9205,1..13))
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cactaaacga gctctgctta tataggcctc ccaccgtaca cgcctacctc gacatacgtt
       61 ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata gggataaact
      121 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
      181 agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg acatcgttct
      241 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
      301 gacatatcga ttcgcggcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt
      361 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
      421 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
      481 aatactgcca tttgtctcga ggtcgagaat tgtcccctcg gggttgggag gtgggtctga
      541 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
      601 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
      661 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
      721 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
      781 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
      841 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
      901 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
      961 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     1021 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     1081 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     1141 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     1201 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     1261 tccctcatat ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt
     1321 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     1381 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     1441 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     1501 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     1561 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     1621 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     1681 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     1741 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     1801 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     1861 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     1921 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     1981 cagagagacc cgtttatgta tcgagctagg cacttaaata caatatctct gcaatgcggc
     2041 aattcagtgg ttcgtccaat ccatgtcaga cccgtctgtt gccttcctaa taaggcacga
     2101 tcgtaccacc ttacttccac caatcggcat gcacggtgct ttttctctcc ttgtaaggca
     2161 tgttgctaac tcatcgttac catgttgcaa gactacaaga gtattgcata agactacatt
     2221 aagcttgcag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     2281 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2341 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2401 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2461 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2521 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2581 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3121 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaaaggc
     4381 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     4441 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     4501 ttaggggcgg gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac
     4561 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     4621 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc
     4681 actctctgat attcatttct ttgcaagtta taaatactga ataataagat gacatgaact
     4741 actactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     4801 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     4861 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     4921 agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag
     4981 tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
     5041 taaaggtacc tctagtccgg acgccgaggc gaaacaggcg gggaggcggc ccaaagggag
     5101 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
     5161 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
     5221 cgcagaatcc aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac
     5281 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
     5341 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
     5401 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
     5461 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
     5521 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
     5581 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaatacgata tcttggatcg
     5641 atccttactt agttacccgg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg
     5701 cagcatatca aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc
     5761 gtcaagggcg tcggtcggcc cgccgctttc gcactttagc tgtttctcca ggccacatat
     5821 gattagttcc aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga acagctcaat
     5881 tgcttgtctc agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc
     5941 tacttgatgc tcctgttcct ccaatacgca gcccagtgta aagtggccca cggcggacag
     6001 agcgtacagt gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc
     6061 cagggtttcg tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg
     6121 atgtgagagg agagcacagc ggtatgactt ggcgttgttc cgcagaaagt cttgccatga
     6181 ctcgccttcc agggggcaga agtgggtatg atgcctgtcc agcatctcga ttggcagggc
     6241 atcgagcagg gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct caactcccag
     6301 cttttgagcg agtttccttg tcgtcaggcc ttcgataccg acaccattga gtaattccag
     6361 agctccgttt atgactttgc tcttgtccag tctagacatt ggaccagggt tttcttcaac
     6421 atcaccacaa gtgaggagag aacctctacc ttcggcaccg ggcttgcggg tcatgcacca
     6481 ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta
     6541 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
     6601 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga
     6661 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc
     6721 ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat
     6781 ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc
     6841 gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc
     6901 ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
     6961 gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc
     7021 gaggcgcacc gtgggcttgt actcggtcat ggtgaattgc tggggagaga ggtcggtgat
     7081 tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga atgcggaaca
     7141 ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc gcaccgcccc
     7201 ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca cagcccatcg
     7261 cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac tagtgagacg
     7321 tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt cccgacttag
     7381 gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga tccgggtgac
     7441 gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc gtttcccgga
     7501 accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa
     7561 ccccaacccc ggatccttag tggtggtggt ggtggtggac cggacgcgtt tacgcataat
     7621 ccggcacatc atacggataa ccggtaacca ctttgtacaa gaaagttggg tttataacca
     7681 tgtaagaaag ctttctttat ctagcttggt ggcagccaaa acagactcca tgtcattaag
     7741 gatgtcagaa ctcaaagctt cctgcagact tggcatcagc tcctctcctt ctatgttcat
     7801 tccatctcct tccagtgttc caaggtccac atttgtccca ggaatggctt caaggtagtc
     7861 tgggaaacgg ttctgctgtg agggcagggt gctttggttg atagtatcac ctgtatccat
     7921 ctcatccaca ctgttcagga agtcatctgg ggttcgaggg acactgtagc tgctcatgct
     7981 tagtccactg tctgtactct catctcgaga gtgataggtg ccactgttaa ggaaaggatc
     8041 tgagctattg gtcgtcattg ttctcaattc ctgagacatc ccgggagaag acactggatt
     8101 ttgagtccca ccatcctgct ccagtgttgg taactggcta cgcagggcta actcctgccg
     8161 aagcagttct tgctgtttca gccgcagcct ctccttctcc atctgcagtt gctgcagtcg
     8221 catctgttgc tgctggttgg agttgctgcc acccatgacg cctccctgtg ggctctgggg
     8281 agccaggggt ggtggctgtt tcactggagc actctgactg attctctggt tcatggcaaa
     8341 acgagggtca agccttgggt ctagccaaga ggtggtcttg ttcttatggt ttatatagta
     8401 aatttctcca tcctgagtca tggcttgttc ccatccatca ggaagaggac ctgaagccga
     8461 gttcatcata ttctgctgca ctggtggact ggtgggggct gtgacgttca tctgggacag
     8521 catggccttc ctggggtcct gccatgttgt tgtctgatcg atgtgattta agaagtatct
     8581 ctgaccagaa gatgtctttg ccatctccca acctgctggc agaggtacat catcaggtat
     8641 ctcaaaagaa gactgtcgaa gatgctgagc tgtgggtgta gctgctgggc cagagactac
     8701 tccagtgggg gtcagtgtcc caggagaaac agctcccaac tgcagagaag ctggagagga
     8761 atgagctcga acatgctgtg gagtcagggc tcctgcagtg cctgcatcag tactggcctg
     8821 tcgggagtgg gatttgggct ccggcggctt gaagaaggcg tcgggcagct tccggagcct
     8881 catgggcacg gtctggggca cgttggccgt cttggggttc atgacggcgt tgaagagcgc
     8941 ctccaggtcg gtctccgagt ccccgcggac gtgcacgatc tgatgcccgg cggggggtgc
     9001 ctgcggcgcc gcctgggtcg ccgcgggtgc cggttgcccg ggtccggacg gcgggccctg
     9061 cccctgcggg ggctgcgaag gcggctgccc ttggccctgg ggggccggtt gaggcggcgg
     9121 ctgctgcccg ggatccatgc caactttttt gtacaaactt gttgatcaat cgatgctagc
     9181 caattctcca ggcgatctga cggtt
//
