LOCUS       sequence-363738-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of YAP1 S127A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D,
            White EA
  TITLE     YAP1 activation by human papillomavirus E7 promotes basal cell 
            identity in squamous epithelia.
  JOURNAL   Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466.
  PUBMED    35170430
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-16"; volume: "11. pii: 75466. doi"; pages: " 
            10.7554/eLife.75466"
COMMENT     Sequence Label: p8327 pLIX YAP1 S127A
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    154..172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    190..208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    261..279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    341..458
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(671..715)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    900..1133
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1626..1751
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1798..1978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1979..2205)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2233..2253)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2233..2251)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2272..2288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2285..2307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2296..2312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2320..2326,2327..2344,2345..2350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2365..2386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2503..2520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2674..3262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2754..2773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3433..4224,4225..4293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4056..4075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4294..4365)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4365..4500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4540..4661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4551..4570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4605..4624
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4733..4966
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5171..5182
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5580..5600
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5640..5648,5649..5684,5685..5687,5688..5723,
                     5724..5726,5727..5762,5763..5768,5769..6386))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6387..6440)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6441..7037)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6521..6541)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7018..7037
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7047..7557)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7110..7128)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7567..7584)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7600..7626)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    7634..7658
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9125..9149)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(join(9178..9205,1..287))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(9181..9205)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctgcttatat aggcctccca ccgtacacgc ctacctcgac atacgttctc tatcactgat
       61 agggagtaaa ctcgacatac gttctctatc actgataggg ataaactcga catacgttct
      121 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
      181 gacatacgtt ctctatcact gatagggagt aaactcgaca tcgttctcta tcactgatag
      241 ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgac atatcgattc
      301 gcggccaaag tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
      361 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
      421 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
      481 gtctcgaggt cgagaattgt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
      541 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
      601 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
      661 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
      721 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
      781 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
      841 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
      901 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
      961 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     1021 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     1081 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     1141 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
     1201 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
     1261 ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc
     1321 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     1381 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     1441 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga
     1501 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     1561 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     1621 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     1681 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     1741 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     1801 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     1861 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     1921 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccgt
     1981 ttatgtatcg agctaggcac ttaaatacaa tatctctgca atgcggcaat tcagtggttc
     2041 gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg taccacctta
     2101 cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca
     2161 tcgttaccat gttgcaagac tacaagagta ttgcataaga ctacattaag cttgcagctc
     2221 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     2281 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2341 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2401 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2461 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2521 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2581 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2641 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2701 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2761 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2821 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2881 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2941 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3001 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3061 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3301 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3361 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3421 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3481 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3541 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3601 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3661 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3721 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3781 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3901 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3961 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4021 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4081 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4321 catttatcag ggttattgtc tcatgagcgg atacatattt gaaaggcctc caaaaaagcc
     4381 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     4441 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     4501 gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg
     4561 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4621 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt
     4681 catttctttg caagttataa atactgaata ataagatgac atgaactact actgctagag
     4741 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     4801 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     4861 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     4921 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     4981 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctct
     5041 agtccggacg ccgaggcgaa acaggcgggg aggcggccca aagggagatc cgactcgtct
     5101 gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa
     5161 ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg
     5221 tggcaacaca ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat
     5281 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
     5341 ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg
     5401 caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc
     5461 tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga
     5521 aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac
     5581 atagcgtaaa aggagcaaca tagttaagaa tacgatatct tggatcgatc cttacttagt
     5641 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg
     5701 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg
     5761 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg
     5821 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtctcaga
     5881 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc
     5941 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg
     6001 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac
     6061 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga
     6121 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg
     6181 gggcagaagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc
     6241 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt
     6301 ttccttgtcg tcaggccttc gataccgaca ccattgagta attccagagc tccgtttatg
     6361 actttgctct tgtccagtct agacattgga ccagggtttt cttcaacatc accacaagtg
     6421 aggagagaac ctctaccttc ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
     6481 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
     6541 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
     6601 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
     6661 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
     6721 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
     6781 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg
     6841 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
     6901 acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac
     6961 gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg
     7021 ggcttgtact cggtcatggt gaattgctgg ggagagaggt cggtgattcg gtcaacgagg
     7081 gagccgactg ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg cgcgggcagg
     7141 aacagggccc acactaccgc cccacacccc gcctcccgca ccgccccttc ccggccgctg
     7201 ctctcggcgc gccctgctga gcagccgcta ttggccacag cccatcgcgg tcggcgcgct
     7261 gccattgctc cctggcgctg tccgtctgcg agggtactag tgagacgtgc ggcttccgtt
     7321 tgtcacgtcc ggcacgccgc gaaccgcaag gaaccttccc gacttagggg cggagcagga
     7381 agcgtcgccg gggggcccac aagggtagcg gcgaagatcc gggtgacgct gcgaacggac
     7441 gtgaagaatg tgcgagaccc agggtcggcg ccgctgcgtt tcccggaacc acgcccagag
     7501 cagccgcgtc cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc caaccccgga
     7561 tccttagtgg tggtggtggt ggtggaccgg acgcgtttac gcataatccg gcacatcata
     7621 cggataaccg gtaaccactt tgtacaagaa agttgggttt ataaccatgt aagaaagctt
     7681 tctttatcta gcttggtggc agccaaaaca gactccatgt cattaaggat gtcagaactc
     7741 aaagcttcct gcagacttgg catcagctcc tctccttcta tgttcattcc atctccttcc
     7801 agtgttccaa ggtccacatt tgtcccagga atggcttcaa ggtagtctgg gaaacggttc
     7861 tgctgtgagg gcagggtgct ttggttgata gtatcacctg tatccatctc atccacactg
     7921 ttcaggaagt catctggggt tcgagggaca ctgtagctgc tcatgcttag tccactgtct
     7981 gtactctcat ctcgagagtg ataggtgcca ctgttaagga aaggatctga gctattggtc
     8041 gtcattgttc tcaattcctg agacatcccg ggagaagaca ctggattttg agtcccacca
     8101 tcctgctcca gtgttggtaa ctggctacgc agggctaact cctgccgaag cagttcttgc
     8161 tgtttcagcc gcagcctctc cttctccatc tgcagttgct gcagtcgcat ctgttgctgc
     8221 tggttggagt tgctgccacc catgacgcct ccctgtgggc tctggggagc caggggtggt
     8281 ggctgtttca ctggagcact ctgactgatt ctctggttca tggcaaaacg agggtcaagc
     8341 cttgggtcta gccaagaggt ggtcttgttc ttatggttta tatagtaaat ttctccatcc
     8401 tgagtcatgg cttgttccca tccatcagga agaggacctg aagccgagtt catcatattc
     8461 tgctgcactg gtggactggt gggggctgtg acgttcatct gggacagcat ggccttcctg
     8521 gggtcctgcc atgttgttgt ctgatcgatg tgatttaaga agtatctctg accagaagat
     8581 gtctttgcca tctcccaacc tgctggcaga ggtacatcat caggtatctc aaaagaagac
     8641 tgtcgaagat gctgagctgt gggtgtagct gctgggccag agactactcc agtgggggtc
     8701 agtgtcccag gagaaacagc tcccaactgc agagaagctg gagaggcatg agctcgaaca
     8761 tgctgtggag tcagggctcc tgcagtgcct gcatcagtac tggcctgtcg ggagtgggat
     8821 ttgggctccg gcggcttgaa gaaggagtcg ggcagcttcc ggagcctcat gggcacggtc
     8881 tggggcacgt tggccgtctt ggggttcatg acggcgttga agagcgcctc caggtcggtc
     8941 tccgagtccc cgcggacgtg cacgatctga tgcccggcgg ggggtgcctg cggcgccgcc
     9001 tgggtcgccg cgggtgccgg ttgcccgggt ccggacggcg ggccctgccc ctgcgggggc
     9061 tgcgaaggcg gctgcccttg gccctggggg gccggttgag gcggcggctg ctgcccggga
     9121 tccatgccaa cttttttgta caaacttgtt gatcaatcga tgctagccaa ttctccaggc
     9181 gatctgacgg ttcactaaac gagct
//
