LOCUS       sequence-363753-        9135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of YAP1 WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D,
            White EA
  TITLE     YAP1 activation by human papillomavirus E7 promotes basal cell 
            identity in squamous epithelia.
  JOURNAL   Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466.
  PUBMED    35170430
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-16"; volume: "11. pii: 75466. doi"; pages: " 
            10.7554/eLife.75466"
COMMENT     Sequence Label: p8325 pLIX YAP1 WT
FEATURES             Location/Qualifiers
     source          1..9135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..50)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(47..85)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    97..115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    133..151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    241..259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    321..438
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(651..695)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    880..1113
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1606..1731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1778..1958
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1959..2185)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2213..2233)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2213..2231)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2252..2268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2252..2268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2265..2287)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2276..2292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2300..2306,2307..2324,2325..2330))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2345..2366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2483..2500)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2654..3242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2734..2753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3413..4204,4205..4273))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4036..4055
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4274..4345)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4345..4480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4520..4641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4531..4550)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4585..4604
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4713..4946
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5151..5162
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5560..5580
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5620..5628,5629..5664,5665..5667,5668..5703,
                     5704..5706,5707..5742,5743..5748,5749..6366))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6367..6420)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6421..7017)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6501..6521)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6998..7017
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7027..7537)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7090..7108)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7547..7564)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7580..7606)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    7614..7638
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9105..9129)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcgatgcta gccaattctc caggcgatct gacggttcac taaacgagct ctgcttatat
       61 aggcctccca ccgtacacgc ctacctcgac atacgttctc tatcactgat agggagtaaa
      121 ctcgacatac gttctctatc actgataggg antaaactcg acatacgttc tctatcactg
      181 atagggagta aactcgacat ncgttctcta tcactgatag ggagtaaact cgacatacgt
      241 tctctatcac tgatagggag taaactcgac atatcgattc gcggccaaag tggatctctg
      301 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
      361 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
      421 cccccttttc ttttaaaatt gtggatgaat actgccattt gtctcgaggt cgagaattgt
      481 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
      541 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
      601 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
      661 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
      721 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
      781 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
      841 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
      901 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
      961 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     1021 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     1081 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     1141 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     1201 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     1261 agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa
     1321 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     1381 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     1441 tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag
     1501 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     1561 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     1621 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     1681 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     1741 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     1801 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     1861 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     1921 agctcccagg ctcagatctg gtctaaccag agagacccgt ttatgtatcg agctaggcac
     1981 ttaaatacaa tatctctgca atgcggcaat tcagtggttc gtccaatcca tgtcagaccc
     2041 gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca
     2101 cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac
     2161 tacaagagta ttgcataaga ctacattaag cttgcagctc cagcttttgt tccctttagt
     2221 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4321 tcatgagcgg atacatattt gaaaggcctc caaaaaagcc tcctcactac ttctggaata
     4381 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     4441 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     4501 tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     4561 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4621 ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg caagttataa
     4681 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     4741 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     4801 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     4861 ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct
     4921 tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc
     4981 tacagctgcc ttgtaagtca ttggtcttaa aggtacctct agtccggacg ccgaggcgaa
     5041 acaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc
     5101 ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc
     5161 gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc
     5221 catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc caacagccga
     5281 gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg
     5341 gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg
     5401 gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc
     5461 tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa
     5521 gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca
     5581 tagttaagaa tacgatatct tggatcgatc cttacttagt tacccgggga gcatgtcaag
     5641 gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc
     5701 ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca
     5761 ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc
     5821 ggctccctgc cggtcgaaca gctcaattgc ttgtctcaga agtgggggca tagaatcggt
     5881 ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc
     5941 cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg agaagccttg
     6001 ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg ttgggcgggt
     6061 gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt atgacttggc
     6121 gttgttccgc agaaagtctt gccatgactc gccttccagg gggcagaagt gggtatgatg
     6181 cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct tcacgtgcca
     6241 gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc
     6301 gataccgaca ccattgagta attccagagc tccgtttatg actttgctct tgtccagtct
     6361 agacattgga ccagggtttt cttcaacatc accacaagtg aggagagaac ctctaccttc
     6421 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
     6481 ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag
     6541 gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc
     6601 cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc
     6661 cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc
     6721 gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc
     6781 cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc
     6841 gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc
     6901 cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac
     6961 ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt
     7021 gaattgctgg ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg
     7081 ctccggaggc ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc
     7141 cccacacccc gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga
     7201 gcagccgcta ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg
     7261 tccgtctgcg agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc
     7321 gaaccgcaag gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac
     7381 aagggtagcg gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc
     7441 agggtcggcg ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa
     7501 cccagggctg ccttggaaaa ggcgcaaccc caaccccgga tccttagtgg tggtggtggt
     7561 ggtggaccgg acgcgtttac gcataatccg gcacatcata cggataaccg gtaaccactt
     7621 tgtacaagaa agttgggttt ataaccatgt aagaaagctt tctttatcta gcttggtggc
     7681 agccaaaaca gactccatgt cattaaggat gtcagaactc aaagcttcct gcagacttgg
     7741 catcagctcc tctccttcta tgttcattcc atctccttcc agtgttccaa ggtccacatt
     7801 tgtcccagga atggcttcaa ggtagtctgg gaaacggttc tgctgtgagg gcagggtgct
     7861 ttggttgata gtatcacctg tatccatctc atccacactg ttcaggaagt catctggggt
     7921 tcgagggaca ctgtagctgc tcatgcttag tccactgtct gtactctcat ctcgagagtg
     7981 ataggtgcca ctgttaagga aaggatctga gctattggtc gtcattgttc tcaattcctg
     8041 agacatcccg ggagaagaca ctggattttg agtcccacca tcctgctcca gtgttggtaa
     8101 ctggctacgc agggctaact cctgccgaag cagttcttgc tgtttcagcc gcagcctctc
     8161 cttctccatc tgcagttgct gcagtcgcat ctgttgctgc tggttggagt tgctgccacc
     8221 catgacgcct ccctgtgggc tctggggagc caggggtggt ggctgtttca ctggagcact
     8281 ctgactgatt ctctggttca tggcaaaacg agggtcaagc cttgggtcta gccaagaggt
     8341 ggtcttgttc ttatggttta tatagtaaat ttctccatcc tgagtcatgg cttgttccca
     8401 tccatcagga agaggacctg aagccgagtt catcatattc tgctgcactg gtggactggt
     8461 gggggctgtg acgttcatct gggacagcat ggccttcctg gggtcctgcc atgttgttgt
     8521 ctgatcgatg tgatttaaga agtatctctg accagaagat gtctttgcca tctcccaacc
     8581 tgctggcaga ggtacatcat caggtatctc aaaagaagac tgtcgaagat gctgagctgt
     8641 gggtgtagct gctgggccag agactactcc agtgggggtc agtgtcccag gagaaacagc
     8701 tcccaactgc agagaagctg gagaggaatg agctcgaaca tgctgtggag tcagggctcc
     8761 tgcagtgcct gcatcagtac tggcctgtcg ggagtgggat ttgggctccg gcggcttgaa
     8821 gaaggagtcg ggcagcttcc ggagcctcat gggcacggtc tggggcacgt tggccgtctt
     8881 ggggttcatg acggcgttga agagcgcctc caggtcggtc tccgagtccc cgcggacgtg
     8941 cacgatctga tgcccggcgg ggggtgcctg cggcgccgcc tgggtcgccg cgggtgccgg
     9001 ttgcccgggt ccggacggcg ggccctgccc ctgcgggggc tgcgaaggcg gctgcccttg
     9061 gccctggggg gccggttgag gcggcggctg ctgcccggga tccatgccaa cttttttgta
     9121 caaacttgtt gatca
//
