LOCUS       sequence-363785-        8073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transposon containing BiFC VC cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, 
            Ashworth A, Lord CJ
  TITLE     Coupling bimolecular PARylation biosensors with genetic screens to 
            identify PARylation targets.
  JOURNAL   Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4.
  PUBMED    29789535
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-22"; volume: "9(1):2016. doi"; pages: " 
            10.1038/s41467-018-04466-4"
COMMENT     Sequence Label: UPATrap-VC-ORF0
FEATURES             Location/Qualifiers
     source          1..8073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2222..2238
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2222..2238
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2272..2283,2284..2471)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    2511..2544
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2664..2697
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(2756..2789)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2966..2999)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3261..3287
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3390..3641
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3578..3599
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3672..3896
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3923..4147
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4815..4848
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5023..5056
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(5102..5135)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             5891..6694
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5954..5973)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6564..6583
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(7034..7067)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    join(7291..7453,7454..7465)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7495..7514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7496..7514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7524..7541)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7524..7540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7533..7555)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7699..7718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7909..7930
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccagctag ctggcggcgg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tgggcccggc tcgatctccg cggccgcatg atcgattgcc gaagttccta
     2521 ttctctagaa agtataggaa cttcggcatc ggcatcaatt catcatcgga tatttgcatc
     2581 gcaatatcaa tccattgccc ggatggccgg atgatcgatg gcggatgcca tgcgatggat
     2641 cggaaatcgc atccattccg gccgaagttc ctattcttca aatagtatag gaacttcggc
     2701 gaattcggaa tcattggcaa tgccatccat cggatttgat gccatgcaat tccggataac
     2761 ttcgtatagc atacattata cgaagttatc cgaaagctgt ccccaatcct cccaccacga
     2821 cccgccgcac tgcagatacc gcaccgtatt ggcaagtagc ccgtaaacgc ggcgaatcgc
     2881 agccagcata gccaggtcca gccgctcgcc ggggcgctgg cgtttggcca ggcggtcgat
     2941 gtgtctgtcc tccggaaggg cccggataac ttcgtatagt acacattata cgaagttatc
     3001 cgggatctgc tgtatctcta agatggctgg cacgtaaaga gggtctcaat aaatatgcct
     3061 tgaattaatg aaaacagtgg tttctgaatc atggcctcca aagagcattt gccttttccc
     3121 ctctcaccct tctgaaggtg cccaggctgc cctggcagga tgcctctttc tctgtggggt
     3181 ggcattctct gctctctctc taatactctt tctttttctt ccctcctctc ccccaactgc
     3241 aggatgcctt tgtggaactg tacccatacg atgttccaga ttacgctctt atggccatgg
     3301 aggcccgaat tcggtcgacc gagatctctc gaggtacccg tccggcgtgc aaaatcccga
     3361 acgacctgaa acagaaagtc atgaaccacg acaagcagaa gaacggcatc aaggccaact
     3421 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     3481 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     3541 ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3601 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcgcc agcacagtgg
     3661 tcgagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3721 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3781 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3841 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3901 tgaggcggaa agctagactc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3961 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4021 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4081 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4141 tctatggctt ctgaggcgga aagcttcagc gatggcttct agctgcccgg gtggcatccc
     4201 tgtgacccct ccccagtgcc tcttctggcc ctggaagttg ccactccagt gcccaccagc
     4261 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     4321 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     4381 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     4441 tccgcctcct gggttcaagc gattctcctg cctcagcctt ctagcttcag cgatggcttc
     4501 tagctgcccg ggtggcatcc ctgtgacccc tccccagtgc ctcttctggc cctggaagtt
     4561 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4621 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     4681 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     4741 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     4801 cccgagtcga gcggataact tcgtataatg tatgctatac gaagttatcc gcgccaggtc
     4861 gcagatcgtc ggtatggagc cgggggtggt gacgtgggtc tggaccatcc cggaggtaag
     4921 ttgcagcagg gcgtcccggc agccggcggg cgattggtcg taatccagga taaagacgtg
     4981 catggaacgg aggcgtttgg ccaagacgtc caaggcccac ggataacttc gtataatgtg
     5041 tactatacga agttatccga tcgatatcca tcccaaatat gcgatggatg gatcggatgc
     5101 cgaagttcct atactttcta gagaatagga acttcggcat tgcggccgca aatagatcca
     5161 tccaatgaag cttgggctga acatcgagcg ccagggctcc gtaaagctac tagagcacag
     5221 gcggtgcccc aacgtcctgg ggcctctcca ctaataacgg ctacttccaa ttgattggac
     5281 gcgccatctt gcctgcctta tgcatattca gcggtgaact gaatattcat gaacgaggcc
     5341 cgtcccgtcc ctccctcctt ccccccaccc ccggaacccg ctccggagga cccgaagggc
     5401 cccgccttca ttaccgatgc gtaggacaaa ccattttccc gatgtgtgtg gggggatact
     5461 aatgagagac tttagctgaa aatgagcctg aactccgaag ctgagtaaaa atggcctaac
     5521 tttatcctcc gttctgtaag tcctcggttt gagtgcacgg gaaacccgaa aggaggacga
     5581 caggaccagg aacattctcc tcctcctgtc gcgtcagaaa gaacacccaa ccagggagcc
     5641 ggagccctag cgtcaacaac tccgccgcgc gcgctccgtg taggccggtg cgggcggccc
     5701 cgtagcgcaa gggagggcgg gaaaggaagg ggcgggacac aagggcgaat ctataaaggg
     5761 cgtcactcag ccagttctct cctcagaagc gccgagagcg cgaccgggac ggttggagaa
     5821 gaaggtggct cccggaaggg ggagagacaa actgccgtaa cctctgccgt tcaggatccg
     5881 tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc
     5941 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6001 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6061 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6121 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6181 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6241 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6301 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6361 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6421 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc
     6481 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6541 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6601 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6661 cgccttctat cgccttcttg acgagttctt ctgaccaccg gtgacgccat ggccaatcgc
     6721 atcatggatt ccatccgatg cgaatcgcat ccatggatgc gccaatcgga ttccatgcga
     6781 tgccatttcc gatgcgggat ccgaaattcc agacaagttt gttgttggat atgccctcga
     6841 ctacaacgag tacttcagga atttgcaggt aagtaatgct tttttttttc tcgctctcat
     6901 ttttcaaaaa cccacataaa aattgaggaa agggaagaat tgttttctcc ttccagcacc
     6961 tcgtaatttg acccgactga tggttcccat tagtcacata aagctgtagt caagtacaga
     7021 cgtcgaattc gccgaagttc ctatactatt tgaagaatag gaacttcggc ctcgagcatg
     7081 catcatatcc atcgcaatcg catccccatt caattcacat cgcatatccg atgccgatat
     7141 ggcatcggca ttgccatcca atcctgatca ccatatatat gatgatatat atatgatgat
     7201 atatatatat tatgatcgcc atccaatttg cgcgaattgc aatccgaatg gatccatgcg
     7261 gatcatcgca tccattgcga taaaggatct ttcttgcttt tacttttact tccttaatac
     7321 tcaagtacaa ttttaatgga gtactttttt acttttactc aagtaagatt ctagccagat
     7381 acttttactt ttaattgagt aaaattttcc ctaagtactt gtactttcac ttgagtaaaa
     7441 tttttgagta ctttttacac ctctgtcaag aaccatatgc cggtacccaa ttcgccctat
     7501 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7561 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7621 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7681 gacgcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7741 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7801 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7861 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7921 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7981 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8041 ttaacaaaat attaacgctt acaatttagg tgg
//
