LOCUS       sequence-36379-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 NusA TEV LIC cloning vector (2N-T)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..121)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcagcgc gcacgcgtgg taccggccgg ccaccaggtg
       61 gcgcgccgcg atcgcggatc cgttatccac ttccaatatt ggattggaag tacaggtttt
      121 cctcgatccc cgcttcgtca ccgaaccagc aaatgttacg ggcagccata atcagtgctc
      181 cggctttttc gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga
      241 gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctaccc
      301 cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct
      361 gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat
      421 caaggccttc gatttccaac agctctttca tcggcacata ggccaattct tccagcgtcg
      481 agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga
      541 aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca
      601 tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc
      661 caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat
      721 ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacgaac tgcgccgggt
      781 tatcatccca caggacgata tcgatacgct cgccacccag ttcagtagac accgcctgaa
      841 cacgcgcgcc acgcatacct acgcaagcac ctaccggatc gatacgttta tcgttggttt
//
