LOCUS       sequence-363819-        7679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of human GST-OSR1-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7679)
  TITLE     Najafov lab plasmids
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEBG-hOSR1-Flag
FEATURES             Location/Qualifiers
     source          1..7679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    112..133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(148..153,154..171,172..178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    186..202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     191..213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     210..226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     210..226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        258..468
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        280..476
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      327..462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        563..1741
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          794..1732
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1689..1709
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1793..2446
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2404..2426
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2453..2470
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             2471..2494
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4103..4126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4813..4830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4813..4829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4822..4844)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5042..5497
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5129..5148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5339..5360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5514..5533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5633..5655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5693..5711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5779..5883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5884..5952,5953..6744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6102..6121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6915..7503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7404..7423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7657..7674
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
       61 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      121 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      181 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      241 gcttaattcc ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      301 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      361 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
      421 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
      481 tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
      541 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      601 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      661 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      721 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      781 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
      841 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
      901 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      961 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     1021 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     1081 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     1141 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     1201 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1261 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1321 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1381 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1441 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1501 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1561 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1621 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1681 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1741 aggaattctc tagagatccc tcgacctcga gatccattgt gctggatcta caatggcccc
     1801 tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc ttttggaata
     1861 tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa
     1921 caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt
     1981 taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg
     2041 tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag
     2101 atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct
     2161 tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt
     2221 aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt
     2281 atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat
     2341 tgaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt
     2401 gcagggctgg caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg atctggttcc
     2461 gcgtggatct ctggaagttc tgttccaggg gcccctggga tccactagtg gtaccatgtc
     2521 cgaggactcg agcgccctgc cctggtccat caacagggac gattacgagc tgcaggaggt
     2581 gatcgggagt ggagcaactg ctgtagtcca agcagcttat tgtgccccta aaaaggagaa
     2641 agtggcaatc aaacggataa accttgagaa atgtcaaact agcatggatg aactcctgaa
     2701 agaaattcaa gccatgagtc aatgccatca tcctaatatt gtatcttact acacatcttt
     2761 tgtggtaaaa gatgagctgt ggcttgtcat gaagctgcta agtggaggtt ctgttctgga
     2821 tattattaag cacattgtgg caaaagggga acacaaaagt ggagtcctag atgaatctac
     2881 cattgctacg atactccgag aagtactgga agggctggaa tatctgcata aaaatggaca
     2941 gatccacaga gatgtgaaag ctggaaacat tcttcttgga gaagatggct cagtacagat
     3001 tgcagacttt ggggttagtg cttttttagc aactggtggt gatattaccc gaaataaagt
     3061 gagaaagacc tttgttggca ccccttgttg gatggcacct gaagttatgg aacaggtccg
     3121 tggttatgat ttcaaagctg atatttggag ttttggaatt acagcaattg aattggctac
     3181 aggggcggct ccttatcata aatatccacc aatgaaggtt ttaatgctga cactgcagaa
     3241 cgatcctcct tctttggaaa ctggtgttca agataaagaa atgctgaaaa aatatggaaa
     3301 atcatttaga aaaatgattt cattgtgcct tcaaaaagat ccagaaaaaa gaccaacagc
     3361 agcagaacta ttaaggcaca aatttttcca gaaagcaaag aataaagaat ttcttcaaga
     3421 aaaaacattg cagagagcac caaccatttc tgaaagagca aaaaaggttc ggagagtacc
     3481 aggttccagt gggcgtcttc ataagacaga ggatggaggc tgggagtgga gtgatgatga
     3541 atttgatgaa gaaagtgagg aagggaaagc agcaatttca caactcaggt ctccccgagt
     3601 gaaagaatca atatcaaatt ctgagctctt tccaacaact gatcctgtgg gtactttgct
     3661 ccaagttcca gaacagatct ctgctcatct acctcagcca gctgggcaga ttgctacaca
     3721 gccaactcaa gtctctctcc cacccaccgc agagccagca aaaacagctc aggctttgtc
     3781 ttcaggatca ggttcacaag aaaccaagat cccaatcagt ctagtactaa gattaaggaa
     3841 ttccaaaaaa gaactaaatg atattcgatt tgaatttact cctgggagag atacagcaga
     3901 gggtgtctct caggaactca tttctgctgg cctggtcgac ggaagggatt tagtaatagt
     3961 ggcagctaat ttgcagaaaa ttgtggaaga acctcagtca aatcgatctg tcactttcaa
     4021 actggcatct ggtgtcgaag gctcagatat tcctgatgat ggtaaactga taggatttgc
     4081 ccagctcagc atcagcggag gagattacaa ggatgacgat gacaagtagg cggccgcgac
     4141 tctagagtga gggtccccac ctgggaccct tgagagtatc aggtctccca cgtgggagac
     4201 aagaaatccc tgtttaatat ttaaacagca gtgttcccca tctgggtcct tgcacccctc
     4261 actctggcct cagccgactg cacagcggcc cctgcatccc cttggctgtg aggcccctgg
     4321 acaagcagag gtggccagag ctgggaggca tggccctggg gtcccacgaa tttgctgggg
     4381 aatctcgttt ttcttcttaa gacttttggg acatggtttg actcccgaac atcaccgacg
     4441 tgtctcctgt ttttctgggt ggcctcggga cacctgccct gcccccacga gggtcaggac
     4501 tgtgactctt tttagggcca ggcaggtgcc tggacatttg ccttgctgga cggggactgg
     4561 ggatgtggga gggagcagac aggaggaatc atgtcaggcc tgtgtgtgaa aggaagctcc
     4621 actgtcaccc tccacctctt caccccccac tcaccagtgt cccctccact gtcacattgt
     4681 aactgaactt caggataata aagwgwttgc ctccaaaaaa aaaaaaaaaa aaaaaaaaaa
     4741 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4801 aaaaccgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4861 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4921 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     4981 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     5041 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5101 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5161 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5221 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     5281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5341 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
     5401 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
     5461 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     5521 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5581 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5641 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5701 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5761 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5821 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5881 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5941 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6001 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6061 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6121 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6181 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6241 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6301 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6361 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6421 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6481 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6541 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6601 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6661 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6721 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6781 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6841 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6901 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6961 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7021 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7081 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7141 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7201 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7261 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7321 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7381 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7441 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7501 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7561 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7621 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagc
//
