LOCUS       sequence-36382-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     mammalian homologous recombination reporter genes
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGCGFP-blcd
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    467..588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(478..497)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     532..551
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(756..777)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cttcctcatc tccgggcctt tcgacctcga gagccgccac catgaaaacc ttcaacatct
       61 ctcagcagga tctggagctg gtggaggtcg ccactgagaa gatcaccatg ctctatgagg
      121 acaacaagca ccatgtcggg gcggccatca ggaccaagac tggggagatc atctctgctg
      181 tccacattga ggcctacatt ggcagggtca ctgtctgtgc tgaagccatt gccattgggt
      241 ctgctgtgag caacgggcag aaggactttg acaccattgt ggctgtcagg cacccctact
      301 ctgatgaggt ggacagatcc atcagggtgg tcagcccctg tggcatgtgc agagagctca
      361 tctctgacta tgctcctgac tgctttgtgc tcattgagat gaatggcaag ctggtcaaaa
      421 ccaccattga ggaactcatc cccctcaagt acaccaggaa ctaacttaag atacattgat
      481 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      541 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      601 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      661 aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac
      721 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      781 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      841 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggta
//
