LOCUS       sequence-363820-        8542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 Delta Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Benlarbi M, Laroche G, Fink C, Fu K, Mulloy RP, Phan A, Ariana A, 
            Stewart CM, Prevost J, Beaudoin-Bussieres G, Daniel R, Bo Y, El 
            Ferri O, Yockell-Lelievre J, Stanford WL, Giguere PM, Mubareka S, 
            Finzi A, Dekaban GA, Dikeakos JD, Cote M
  TITLE     Identification and differential usage of a host metalloproteinase 
            entry pathway by SARS-CoV-2 Delta and Omicron.
  JOURNAL   iScience. 2022 Nov 18;25(11):105316. doi: 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10.
  PUBMED    36254158
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-18"; volume: "25(11):105316. doi"; pages: " 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 Delta Spike
FEATURES             Location/Qualifiers
     source          1..8542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5563..5582)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5628..5683
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5682..5701)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6044..6060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6044..6060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6057..6079)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6068..6084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6092..6098,6099..6116,6117..6122))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6137..6158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6217..6412
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6263..6398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6418..6552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6455..6474)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6509..6528
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6620..6637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6791..7379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6871..6890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7550..8341,8342..8410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8173..8192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8411..8515)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg sggggsgggg sgrggggsgg
      541 ggsggggsgr ggcggrgagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccggggr gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cgccaccatg
     1741 ttcgtcttcc tcgtcctcct ccccctcgtc tcctcccagt gcgtcaacct cagaacccgc
     1801 acccagctcc cccccgccta caccaactcc ttcacccgcg gcgtctacta ccccgacaag
     1861 gtcttccgct cctccgtcct ccactccacc caggacctct tcctcccctt cttctccaac
     1921 gtcacctggt tccacgccat ccacgtctcc ggcaccaacg gcaccaagcg cttcgacaac
     1981 cccgtcctcc ccttcaacga cggcgtctac ttcgcctcca ccgagaagtc caacatcatc
     2041 cgcggctgga tcttcggcac caccctcgac tccaagaccc agtccctcct catcgtcaac
     2101 aacgccacca acgtcgtcat caaggtctgc gagttccagt tctgcaacga ccccttcctc
     2161 ggcgtctact accacaagaa caacaagtcc tggatggagt ccggcgtcta ctcctccgcc
     2221 aacaactgca ccttcgagta cgtctcccag cccttcctca tggacctcga gggcaagcag
     2281 ggcaacttca agaacctccg cgagttcgtc ttcaagaaca tcgacggcta cttcaagatc
     2341 tactccaagc acacccccat caacctcgtc cgcgacctcc cccagggctt ctccgccctc
     2401 gagcccctcg tcgacctccc catcggcatc aacatcaccc gcttccagac cctcctcgcc
     2461 ctccaccgct cctacctcac ccccggcgac tcctcctccg gctggaccgc cggcgccgcc
     2521 gcctactacg tcggctacct ccagccccgc accttcctcc tcaagtacaa cgagaacggc
     2581 accatcaccg acgccgtcga ctgcgccctc gaccccctct ccgagaccaa gtgcaccctc
     2641 aagtccttca ccgtcgagaa gggcatctac cagacctcca acttccgcgt ccagcccacc
     2701 gagtccatcg tccgcttccc caacatcacc aacctctgcc ccttcggcga ggtcttcaac
     2761 gccacccgct tcgcctccgt ctacgcctgg aaccgcaagc gcatctccaa ctgcgtcgcc
     2821 gactactccg tcctctacaa ctccgcctcc ttctccacct tcaagtgcta cggcgtctcc
     2881 cccaccaagc tcaacgacct ctgcttcacc aacgtctacg ccgactcctt cgtcatccgc
     2941 ggcgacgagg tccgccagat cgcccccggc cagaccggca agatcgccga ctacaactac
     3001 aagctccccg acgacttcac cggctgcgtc atcgcctgga actccaacaa cctcgactcc
     3061 aaggtcggcg gcaactacaa ctaccgctac cgcctcttcc gcaagtccaa cctcaagccc
     3121 ttcgagcgcg acatctccac cgagatctac caggccggct ccaagccctg caacggtgtc
     3181 gagggcttca actgctactt ccccctccag tcctacggct tccagcccac caacggcgtc
     3241 ggctaccagc cctaccgcgt cgtcgtcctc tccttcgagc tcctccacgc ccccgccacc
     3301 gtctgcggcc ccaagaagtc caccaacctc gtcaagaaca agtgcgtcaa cttcaacttc
     3361 aacggcctca ccggcaccgg cgtcctcacc gagtccaaca agaagttcct ccccttccag
     3421 cagttcggcc gcgacatcgc cgacaccacc gacgccgtcc gcgaccccca gaccctcgag
     3481 atcctcgaca tcaccccctg ctccttcggc ggcgtctccg tcatcacccc cggcaccaac
     3541 acctccaacc aggtcgccgt cctctaccag ggtgtcaact gcaccgaggt ccccgtcgcc
     3601 atccacgccg accagctcac ccccacctgg cgcgtctact ccaccggctc caacgtcttc
     3661 cagacccgcg ccggctgcct catcggcgcc gagcacgtca acaactccta cgagtgcgac
     3721 atccccatcg gcgccggcat ctgcgcctcc taccagaccc agaccaactc cagacgccgc
     3781 gcccgctccg tcgcctccca gtccatcatc gcctacacca tgtccctcgg cgccgagaac
     3841 tccgtcgcct actccaacaa ctccatcgcc atccccacca acttcaccat ctccgtcacc
     3901 accgagatcc tccccgtctc catgaccaag acctccgtcg actgcaccat gtacatctgc
     3961 ggcgactcca ccgagtgctc caacctcctc ctccagtacg gctccttctg cacccagctc
     4021 aaccgcgccc tcaccggcat cgccgtcgag caggacaaga acacccagga ggtcttcgcc
     4081 caggtcaagc agatctacaa gacccccccc atcaaggact tcggcggctt caacttctcc
     4141 cagatcctcc ccgacccctc caagccctcc aagcgctcct tcatcgagga cctcctcttc
     4201 aacaaggtca ccctcgccga cgccggcttc atcaagcagt acggcgactg cctcggcgac
     4261 atcgccgccc gcgacctcat ctgcgcccag aagttcaacg gcctcaccgt cctccccccc
     4321 ctcctcaccg acgagatgat cgcccagtac acctccgccc tcctcgccgg caccatcacc
     4381 tccggctgga ccttcggcgc cggcgccgcc ctccagatcc ccttcgccat gcagatggcc
     4441 taccgcttca acggcatcgg cgtcacccag aacgtcctct acgagaacca gaagctcatc
     4501 gccaaccagt tcaactccgc catcggcaag atccaggact ccctctcctc caccgcctcc
     4561 gccctcggca agctccagaa cgtcgtcaac cagaacgccc aggccctcaa caccctcgtc
     4621 aagcagctct cctccaactt cggcgccatc tcctccgtcc tcaacgacat cctctcccgc
     4681 ctcgacaagg tcgaggccga ggtccagatc gaccgcctca tcaccggccg cctccagtcc
     4741 ctccagacct acgtcaccca gcagctcatc cgcgccgccg agatccgcgc ctccgccaac
     4801 ctcgccgcca ccaagatgtc cgagtgcgtc ctcggccagt ccaagcgcgt cgacttctgc
     4861 ggcaagggct accacctcat gtccttcccc cagtccgccc cccacggcgt cgtcttcctc
     4921 cacgtcacct acgtccccgc ccaggagaag aacttcacca ccgcccccgc catctgccac
     4981 gacggcaagg cccacttccc ccgcgagggc gtcttcgtct ccaacggcac ccactggttc
     5041 gtcacccagc gcaacttcta cgagccccag atcatcacca ccgacaacac cttcgtctcc
     5101 ggcaactgcg acgtcgtcat cggcatcgtc aacaacaccg tctacgaccc cctccagccc
     5161 gagctcgact ccttcaagga ggagctcgac aagtacttca agaaccacac ctcccccgac
     5221 gtcgacctcg gcgacatctc cggcatcaac gcctccgtcg tcaacatcca gaaggagatc
     5281 gaccgcctca acgaggtcgc caagaacctc aacgagtccc tcatcgacct ccaggagctc
     5341 ggcaagtacg agcagtacat caagtggccc tggtacatct ggctcggctt catcgccggc
     5401 ctcatcgcca tcgtcatggt caccatcatg ctctgctgca tgacctcctg ctgctcctgc
     5461 ctcaagggct gctgctcctg cggctcctgc tgcaagttcg acgaggacga ctccgagccc
     5521 gtcctcaagg gcgtcaagct ccactacacc taagctagca gatctttttc cctctgccaa
     5581 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     5641 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     5701 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     5761 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     5821 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     5881 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     5941 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     6001 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     6061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6181 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     6241 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6301 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6361 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     6421 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6481 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6541 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7501 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7561 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7621 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7681 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7741 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7801 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7861 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7921 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7981 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8041 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8101 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8161 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8221 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8281 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8341 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8401 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8521 ttccccgaaa agtgccacct gg
//
