LOCUS       sequence-363823-        8533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Tauzin A, Nicolas A, Ding S, Benlarbi M, Medjahed H, Chatterjee D, 
            Dionne K, Gong SY, Gendron-Lepage G, Bo Y, Perreault J, Goyette G, 
            Gokool L, Arlotto P, Morrisseau C, Tremblay C, Martel-Laferriere V, 
            De Serres G, Levade I, Kaufmann DE, Cote M, Bazin R, Finzi A
  TITLE     Spike recognition and neutralization of SARS-CoV-2 Omicron 
            subvariants elicited after the third dose of mRNA vaccine.
  JOURNAL   Cell Rep. 2023 Jan 31;42(1):111998. doi: 
            10.1016/j.celrep.2023.111998. Epub 2023 Jan 9.
  PUBMED    36656710
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-01-31"; volume: "42(1):111998. doi"; pages: " 
            10.1016/j.celrep.2023.111998. Epub 2023 Jan 9"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 BA.4/5 Spike
FEATURES             Location/Qualifiers
     source          1..8533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5554..5573)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5619..5674
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5673..5692)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6035..6051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6035..6051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6048..6070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6059..6075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6083..6089,6090..6107,6108..6113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6128..6149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6208..6403
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6254..6389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6409..6543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6446..6465)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6500..6519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6611..6628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6782..7370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6862..6881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7541..8332,8333..8401))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8164..8183
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8402..8506)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg sggggsgggg sgrggggsgg
      541 ggsggggsgr ggsggrgrgg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccggggr gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cgccaccatg
     1741 ttcgtcttcc tcgtcctcct ccccctcgtc tcctcccagt gcgtcaacct catcacccgc
     1801 acccagagct acaccaactc cttcacccgc ggcgtctact accccgacaa ggtcttccgc
     1861 tcctccgtcc tccactccac ccaggacctc ttcctcccct tcttctccaa cgtcacctgg
     1921 ttccacgcca tctccggcac caacggcacc aagcgcttcg acaaccccgt cctccccttc
     1981 aacgacggcg tctacttcgc ctccaccgag aagtccaaca tcatccgcgg ctggatcttc
     2041 ggcaccaccc tcgactccaa gacccagtcc ctcctcatcg tcaacaacgc caccaacgtc
     2101 gtcatcaagg tctgcgagtt ccagttctgc aacgacccct tcctcgacgt ctactaccac
     2161 aagaacaaca agtcctggat ggagtccgag ttccgcgtct actcctccgc caacaactgc
     2221 accttcgagt acgtctccca gcccttcctc atggacctcg agggcaagca gggcaacttc
     2281 aagaacctcc gcgagttcgt cttcaagaac atcgacggct acttcaagat ctactccaag
     2341 cacaccccca tcaacctcgg ccgcgacctc ccccagggct tctccgccct cgagcccctc
     2401 gtcgacctcc ccatcggcat caacatcacc cgcttccaga ccctcctcgc cctccaccgc
     2461 tcctacctca cccccggcga ctcctcctcc ggctggaccg ccggcgccgc cgcctactac
     2521 gtcggctacc tccagccccg caccttcctc ctcaagtaca acgagaacgg caccatcacc
     2581 gacgccgtcg actgcgccct cgaccccctc tccgagacca agtgcaccct caagtccttc
     2641 accgtcgaga agggcatcta ccagacctcc aacttccgcg tccagcccac cgagtccatc
     2701 gtccgcttcc ccaacatcac caacctctgc cccttcgacg aggtcttcaa cgccacccgc
     2761 ttcgcctccg tctacgcctg gaaccgcaag cgcatctcca actgcgtcgc cgactactcc
     2821 gtcctctaca acttcgcccc tttcttcgcc ttcaagtgct acggcgtctc ccccaccaag
     2881 ctcaacgacc tctgcttcac caacgtctac gccgactcct tcgtcatccg cggcaacgag
     2941 gtcagccaga tcgcccccgg ccagaccggc aacatcgccg actacaacta caagctcccc
     3001 gacgacttca ccggctgcgt catcgcctgg aactccaaca agctcgactc caaggtcggc
     3061 ggcaactaca actacaggta ccgcctcttc cgcaagtcca acctcaagcc cttcgagcgc
     3121 gacatctcca ccgagatcta ccaggccggc aacaaaccct gcaacggcgt cgccggcgtg
     3181 aactgctact tccccctcca gtcctacggc ttcaggccca cctacggcgt cggccaccag
     3241 ccctaccgcg tcgtcgtcct ctccttcgag ctcctccacg cccccgccac cgtctgcggc
     3301 cccaagaagt ccaccaacct cgtcaagaac aagtgcgtca acttcaactt caacggcctc
     3361 accggcaccg gcgtcctcac cgagtccaac aagaagttcc tccccttcca gcagttcggc
     3421 cgcgacatcg ccgacaccac cgacgccgtc cgcgaccccc agaccctcga gatcctcgac
     3481 atcaccccct gctccttcgg cggcgtctcc gtcatcaccc ccggcaccaa cacctccaac
     3541 caggtcgccg tcctctacca gggtgtcaac tgcaccgagg tccccgtcgc catccacgcc
     3601 gaccagctca cccccacctg gcgcgtctac tccaccggct ccaacgtctt ccagacccgc
     3661 gccggctgcc tcatcggcgc cgagtacgtc aacaactcct acgagtgcga catccccatc
     3721 ggcgccggca tctgcgcctc ctaccagacc cagaccaagt cccaccgccg cgcccgctcc
     3781 gtcgcctccc agtccatcat cgcctacacc atgtccctcg gcgccgagaa ctccgtcgcc
     3841 tactccaaca actccatcgc catccccacc aacttcacca tctccgtcac caccgagatc
     3901 ctccccgtct ccatgaccaa gacctccgtc gactgcacca tgtacatctg cggcgactcc
     3961 accgagtgct ccaacctcct cctccagtac ggctccttct gcacccagct caagcgcgcc
     4021 ctcaccggca tcgccgtcga gcaggacaag aacacccagg aggtcttcgc ccaggtcaag
     4081 cagatctaca agaccccccc catcaagtac ttcggcggct tcaacttctc ccagatcctc
     4141 cccgacccct ccaagccctc caagcgctcc ttcatcgagg acctcctctt caacaaggtc
     4201 accctcgccg acgccggctt catcaagcag tacggcgact gcctcggcga catcgccgcc
     4261 cgcgacctca tctgcgccca gaagttcaac ggcctcaccg tcctcccccc cctcctcacc
     4321 gacgagatga tcgcccagta cacctccgcc ctcctcgccg gcaccatcac ctccggctgg
     4381 accttcggcg ccggcgccgc cctccagatc cccttcgcca tgcagatggc ctaccgcttc
     4441 aacggcatcg gcgtcaccca gaacgtcctc tacgagaacc agaagctcat cgccaaccag
     4501 ttcaactccg ccatcggcaa gatccaggac tccctctcct ccaccgcctc cgccctcggc
     4561 aagctccagg acgtcgtcaa ccacaacgcc caggccctca acaccctcgt caagcagctc
     4621 tcctccaagt tcggcgccat ctcctccgtc ctcaacgaca tcctgtcccg cctcgacaag
     4681 gtcgaggccg aggtccagat cgaccgcctc atcaccggcc gcctccagtc cctccagacc
     4741 tacgtcaccc agcagctcat ccgcgccgcc gagatccgcg cctccgccaa cctcgccgcc
     4801 accaagatgt ccgagtgcgt cctcggccag tccaagcgcg tcgacttctg cggcaagggc
     4861 taccacctca tgtccttccc ccagtccgcc ccccacggcg tcgtcttcct ccacgtcacc
     4921 tacgtccccg cccaggagaa gaacttcacc accgcccccg ccatctgcca cgacggcaag
     4981 gcccacttcc cccgcgaggg cgtcttcgtc tccaacggca cccactggtt cgtcacccag
     5041 cgcaacttct acgagcccca gatcatcacc accgacaaca ccttcgtctc cggcaactgc
     5101 gacgtcgtca tcggcatcgt caacaacacc gtctacgacc ccctccagcc cgagctcgac
     5161 tccttcaagg aggagctcga caagtacttc aagaaccaca cctcccccga cgtcgacctc
     5221 ggcgacatct ccggcatcaa cgcctccgtc gtcaacatcc agaaggagat cgaccgcctc
     5281 aacgaggtcg ccaagaacct caacgagtcc ctcatcgacc tccaggagct cggcaagtac
     5341 gagcagtaca tcaagtggcc ctggtacatc tggctcggct tcatcgccgg cctcatcgcc
     5401 atcgtcatgg tcaccatcat gctctgctgc atgacctcct gctgctcctg cctcaagggc
     5461 tgctgctcct gcggctcctg ctgcaagttc gacgaggacg actccgagcc cgtcctcaag
     5521 ggcgtcaagc tccactacac ctaagctagc agatcttttt ccctctgcca aaaattatgg
     5581 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     5641 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     5701 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     5761 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     5821 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     5881 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     5941 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     6001 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6061 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6181 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     6241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     6421 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6481 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6541 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8521 aagtgccacc tgg
//
