LOCUS       sequence-363824-        4256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Subcloning YAP1 S94A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D,
            White EA
  TITLE     YAP1 activation by human papillomavirus E7 promotes basal cell 
            identity in squamous epithelia.
  JOURNAL   Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466.
  PUBMED    35170430
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-16"; volume: "11. pii: 75466. doi"; pages: " 
            10.7554/eLife.75466"
COMMENT     Sequence Label: p8322 pDONR ATG-YAP1 S94A-Stop
FEATURES             Location/Qualifiers
     source          1..4256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     376..393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(552..579)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(671..757)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     806..828
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     820..837
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     821..837
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    853..952
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2419..2518)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2535..2554)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2536..2554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2559..2575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2559..2575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3006..3797
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3890..4256,1..222)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      481 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
      541 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
      601 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
      661 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      721 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      781 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
      841 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      901 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg catggatccc
      961 gggcagcagc cgccgcctca accggccccc cagggccaag ggcagccgcc ttcgcagccc
     1021 ccgcaggggc agggcccgcc gtccggaccc gggcaaccgg cacccgcggc gacccaggcg
     1081 gcgccgcagg caccccccgc cgggcatcag atcgtgcacg tccgcgggga ctcggagacc
     1141 gacctggagg cgctcttcaa cgccgtcatg aaccccaaga cggccaacgt gccccagacc
     1201 gtgcccatga ggctccggaa gctgcccgac gccttcttca agccgccgga gcccaaatcc
     1261 cactcccgac aggccagtac tgatgcaggc actgcaggag ccctgactcc acagcatgtt
     1321 cgagctcatt cctctccagc ttctctgcag ttgggagctg tttctcctgg gacactgacc
     1381 cccactggag tagtctctgg cccagcagct acacccacag ctcagcatct tcgacagtct
     1441 tcttttgaga tacctgatga tgtacctctg ccagcaggtt gggagatggc aaagacatct
     1501 tctggtcaga gatacttctt aaatcacatc gatcagacaa caacatggca ggaccccagg
     1561 aaggccatgc tgtcccagat gaacgtcaca gcccccacca gtccaccagt gcagcagaat
     1621 atgatgaact cggcttcagg tcctcttcct gatggatggg aacaagccat gactcaggat
     1681 ggagaaattt actatataaa ccataagaac aagaccacct cttggctaga cccaaggctt
     1741 gaccctcgtt ttgccatgaa ccagagaatc agtcagagtg ctccagtgaa acagccacca
     1801 cccctggctc cccagagccc acagggaggc gtcatgggtg gcagcaactc caaccagcag
     1861 caacagatgc gactgcagca actgcagatg gagaaggaga ggctgcggct gaaacagcaa
     1921 gaactgcttc ggcaggagtt agccctgcgt agccagttac caacactgga gcaggatggt
     1981 gggactcaaa atccagtgtc ttctcccggg atgtctcagg aattgagaac aatgacgacc
     2041 aatagctcag atcctttcct taacagtggc acctatcact ctcgagatga gagtacagac
     2101 agtggactaa gcatgagcag ctacagtgtc cctcgaaccc cagatgactt cctgaacagt
     2161 gtggatgaga tggatacagg tgatactatc aaccaaagca ccctgccctc acagcagaac
     2221 cgtttcccag actaccttga agccattcct gggacaaatg tggaccttgg aacactggaa
     2281 ggagatggaa tgaacataga aggagaggag ctgatgccaa gtctgcagga agctttgagt
     2341 tctgacatcc ttaatgacat ggagtctgtt ttggctgcca ccaagctaga taaagaaagc
     2401 tttcttacat ggttataaac ccaactttct tgtacaaagt tggcattata agaaagcatt
     2461 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2521 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2581 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2641 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2701 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2761 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2821 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2881 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2941 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3001 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3061 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3121 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3181 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3241 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3301 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3361 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3421 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3481 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3541 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3601 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3661 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3721 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3781 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3841 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacacc
//
