LOCUS       sequence-363830-        7316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HPV18 E7 Delta-DLLC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D,
            White EA
  TITLE     YAP1 activation by human papillomavirus E7 promotes basal cell 
            identity in squamous epithelia.
  JOURNAL   Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466.
  PUBMED    35170430
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-16"; volume: "11. pii: 75466. doi"; pages: " 
            10.7554/eLife.75466"
COMMENT     Sequence Label: p8291 MSCV-Neo C-HA 18E7 Delta-DLLC
FEATURES             Location/Qualifiers
     source          1..7316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(137..241)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    807..1148
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1215..1631
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1561..1583
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1599..1615
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1643..1662
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1643..1661
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1691..1715
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2019..2043)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2090..2116
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2090..2116
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2129..2628
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2164..2187)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2561..2580
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2649..3443
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2649..3443
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2703..2722)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3313..3332
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             3522..4036
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4198..4214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4222..4228,4229..4246,4247..4252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4267..4288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4405..4422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4576..5164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4656..4675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5335..6126,6127..6195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5958..5977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6196..6300)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6368..6386
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6424..6446)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6546..6565
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6759..6781
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6909..6928
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
       61 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      241 gcacatttcc ccgaaaagtg ccacctgcag cctgaatatg ggccaaacag gatatctgtg
      301 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
      361 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
      421 cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
      481 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
      541 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
      601 gccagtcctc cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct
      661 gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga ttgattgact
      721 gcccacctcg ggggtctttc atttggaggt tccaccgaga tttggagacc cctgcccagg
      781 gaccaccgac ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg tctgtctctg
      841 tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac tagttagcta
      901 actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca
      961 accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg
     1021 agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa
     1081 aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt
     1141 gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     1201 tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga
     1261 aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta
     1321 ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta
     1381 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
     1441 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
     1501 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
     1561 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
     1621 ctctaggcgc cggaattaga tctaatacga ctcactatag ggagacccaa gctggctagt
     1681 taagctatca acaagtttgt acaaaaaagt tggcaccatg catggaccta aggcaacatt
     1741 gcaagacatt gtattgcatt tagagcccca aaatgaaatt ccggttcacg agcaattaag
     1801 cgactcagag gaagaaaacg atgaaataga tggagttaat catcaacatt taccagcccg
     1861 acgagccgaa ccacaacgtc acacaatgtt gtgtatgtgt tgtaagtgtg aagccagaat
     1921 tgagctagta gtagaaagct cagcagacga ccttcgagca ttccagcagc tgtttctgaa
     1981 caccctgtcc tttgtgtgtc cgtggtgtgc atcccagcag ccaactttct tgtacaaagt
     2041 ggttgatcta gagggcccgc ggttcgaact cgagtcggcc gctggaggat acccctacga
     2101 cgtgcccgac tacgcctaga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     2161 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     2221 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     2281 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     2341 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     2401 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     2461 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     2521 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     2581 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     2641 agcttaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     2701 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     2761 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     2821 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     2881 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     2941 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3001 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     3061 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3121 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     3181 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3241 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3301 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3361 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3421 gccttcttga cgagttcttc tgacgcccga ccgaaaggag cgcacgaccc catgcatcga
     3481 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     3541 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag
     3601 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     3661 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     3721 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     3781 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     3841 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     3901 cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     3961 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     4021 agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag
     4081 gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa
     4141 tactcctgaa atagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg
     4201 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4261 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4321 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4381 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccttgg
     4681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4981 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6301 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6361 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6421 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6481 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6541 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6601 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     6661 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     6721 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     6781 gacggcgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     6841 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     6901 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     6961 ccggccacga tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca
     7021 gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag
     7081 ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     7141 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
     7201 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
     7261 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatg
//
