LOCUS       sequence-363839-        8573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of  WT human  MOV10 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C
  TITLE     Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts 
            retrotransposition.
  JOURNAL   EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458.
  PUBMED    35856394
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 20:e54458. doi: 10.15252/embr.202154458."
COMMENT     Sequence Label: T10-T7-hMOV10
FEATURES             Location/Qualifiers
     source          1..8573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        131..510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        511..714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     665..685
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     759..778
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        759..777
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             891..923
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    3600..3611
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(4060..4077)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4066..4290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4336..4764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4423..4442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4633..4654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4773..4793)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4778..5107
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4958..5093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5020..5039
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5174..5968
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5228..5247)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5838..5857
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6142..6263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6179..6198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6233..6252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6312..6328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6312..6328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6325..6347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6336..6352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6360..6366,6367..6384,6385..6390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6405..6426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6543..6560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6714..7302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6794..6813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7473..8264,8265..8333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8096..8115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8334..8438)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8513..8532)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
       61 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      121 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      181 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      241 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      301 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      361 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      421 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      481 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      541 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      601 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      661 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      721 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      781 acccaagctg gctagcgttt aaacttaagc ttaccatgga tctcccagac gatcattacc
      841 tgtccaccca gacaatcctg agcaaagatc ttaatggggt accaggttca atggctagca
      901 tgactggtgg acagcaaatg ggtcccggga gcggcggtga gggctcagcc ggcggaggac
      961 cggtcggagg cggatccatg cccagtaagt tcagctgccg gcagctccgg gaggcgggcc
     1021 agtgtttcga gagtttcctg gtcgttcggg gactggacat ggagacagat cgcgagcggc
     1081 tgcggaccat ttataaccgc gacttcaaga tcagctttgg gacccccgcc cctggcttct
     1141 cctccatgct gtatggaatg aagattgcaa atctggccta cgtcaccaag actcgggtca
     1201 ggttcttcag actcgaccgc tgggccgacg tgcggttccc agaaaagagg agaatgaagc
     1261 tggggtcaga tatcagcaaa caccacaagt cactgctagc caagatcttt tatgacaggg
     1321 ctgagtatct tcatgggaaa catggtgtgg atgtggaagt ccaggggccc catgaagccc
     1381 gagatgggca gctccttatc cgcctggatt tgaaccgcaa agaggtgctg accctgaggc
     1441 ttcggaatgg cggaacccag tctgttaccc tcactcacct cttcccactc tgccggacac
     1501 cccagtttgc tttctacaat gaagaccagg agttgccctg tccactgggc cccggtgaat
     1561 gctatgaact ccatgtccat tgtaagacca gctttgtggg ctacttccca gccacagtgc
     1621 tctgggagct gctgggacct ggggagtcgg gttcagaagg agccggcaca ttctacattg
     1681 cccgcttctt ggctgccgtc gcccacagcc ccctggctgc acagctgaag cccatgactc
     1741 ccttcaagcg gacccggatc accggaaacc ctgtggtgac caatcggata gaggaaggag
     1801 agagacctga ccgcgctaag ggctatgacc tggagttaag tatggcgctg gggacatact
     1861 acccacctcc ccgcctcagg cagctgctcc ccatgcttct tcagggaaca agtatcttca
     1921 ctgcccctaa ggagatcgca gagatcaagg cccagctgga gacagccctg aagtggagga
     1981 actatgaggt gaagctgcgg ctgctgctgc acctggagga actgcagatg gagcatgata
     2041 tccggcacta tgacctggag tcggtgccca tgacctggga ccctgtggac cagaacccca
     2101 ggctgctcac gctggaggtt cctggagtga ctgagagccg cccctcagtg ctacggggcg
     2161 accacctgtt tgcccttttg tcctcggaga cacaccagga ggaccccatc acatataagg
     2221 gctttgtgca caaggtggaa ttggaccgtg tcaagctgag cttttccatg agcctcctga
     2281 gccgctttgt ggatgggctg accttcaagg tgaactttac cttcaaccgc cagccgctgc
     2341 gagtccagca ccgtgccctg gagctgacag ggcgctggct gctgtggccc atgctctttc
     2401 ctgtggcacc tcgggacgtc ccgctgctgc cctcagatgt gaaactcaag ctgtacgacc
     2461 ggagtctgga gtcaaaccca gagcagctgc aggccatgag gcacattgtt acgggcacca
     2521 cccgtccagc cccctacatc atctttgggc ctccaggcac cggcaagact gtcacgttag
     2581 tggaggcaat taagcaggtg gtgaagcact tgcccaaagc ccacatcttg gcctgcgctc
     2641 catccaactc aggggctgac ctactctgtc aaaggctccg ggtccacctt cctagctcca
     2701 tctaccgcct cctggccccc agcagggaca tccgcatggt acctgaggac atcaagccct
     2761 gctgcaactg ggacgcaaag aagggggagt atgtatttcc cgccaagaag aagctgcagg
     2821 aataccgggt cttaattacc accctcatca ctgccggcag gttggtctcg gcccagtttc
     2881 ccattgatca cttcacacac atcttcatcg atgaggctgg ccactgcatg gagcctgaga
     2941 gtctggtagc tatagcaggg ctgatggaag taaaggaaac aggtgatcca ggagggcagc
     3001 tggtgctggc aggagaccct cggcagctgg ggcctgtgct gcgttcccca ctgacccaga
     3061 agcatggact gggatactca ctgctggagc ggctgctcac ctacaactcc ctgtacaaga
     3121 agggccctga tggctatgac ccccagttca taaccaagct gctccgcaac tacaggtctc
     3181 atcccaccat cctggacatt cctaaccagc tctattatga aggggagctg caggcctgtg
     3241 ctgatgtcgt ggatcgagaa cgcttctgcc gctgggcggg cctacctcga cagggctttc
     3301 ccatcatctt tcacggcgta atgggcaaag atgagcgtga aggcaacagc ccatccttct
     3361 tcaaccctga agaggctgcc acagtgactt cctacctgaa gctgctcctg gccccctcct
     3421 ccaagaaggg caaagctcgc ctgagccctc gaagtgtggg cgtcatctcc ccgtaccgga
     3481 aacaggtgga gaaaatccgt tactgcatca ccaaacttga cagggagctt cgaggactgg
     3541 atgacatcaa ggacttgaag gtgggttcag tagaagaatt ccaaggccaa gaacgaagcg
     3601 tcatcctcat ctccaccgtg cgaagcagcc agagctttgt gcagctggat ctggacttta
     3661 atctgggttt ccttaagaac cccaagaggt tcaatgtagc tgtgacccgg gccaaggccc
     3721 tgctcatcat cgtggggaac ccccttctcc tgggccatga ccctgactgg aaagtattcc
     3781 tggagttctg taaagaaaac ggagggtata ccgggtgtcc cttccctgcc aaactggacc
     3841 tgcaacaggg acagaattta ctgcaaggtc tgagcaagct cagcccctct acctcagggc
     3901 cccacagcca tgactacctc ccccaggagc gggagggtga agggggcctg tctctgcaag
     3961 tggagccaga gtggaggaat gagctctgag aattctgcag atatccagca cagtggcggc
     4021 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4081 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4141 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4201 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4261 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4321 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4381 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4441 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4501 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4561 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4621 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4681 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4741 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     4801 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4861 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4921 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4981 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5041 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5101 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     5161 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5221 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5281 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5341 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5401 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5461 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5521 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5581 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5641 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     5701 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     5761 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5821 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5881 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5941 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6001 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6061 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6121 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6241 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8461 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     8521 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gag
//
