LOCUS       sequence-363844-       11697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deletion of BHLH domain of mouse TFAP4 in combination with 
            TFAP4_BHLH_3-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Schaefer M, Nabih A, Spies D, Hermes V, Bodak M, Wischnewski H, 
            Stalder P, Ngondo RP, Liechti LA, Sajic T, Aebersold R, Gatfield D, 
            Ciaudo C
  TITLE     Global and precise identification of functional miRNA targets in 
            mESCs by integrative analysis.
  JOURNAL   EMBO Rep. 2022 Jul 28:e54762. doi: 10.15252/embr.202254762.
  PUBMED    35899551
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 28:e54762. doi: 10.15252/embr.202254762."
COMMENT     Sequence Label: TFAP4_BHLH_5-2
FEATURES             Location/Qualifiers
     source          1..11697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    13..29
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18..40
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     37..53
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     37..53
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     72..92
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        74..92
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        120..346
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             347..527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    574..699
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1192..1425
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1610..1654
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1832..2080
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1832..1852
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2003..2022
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2102..2177
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2225..2342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2411..2622
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2642..2651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2651..2674
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2681..2701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2726..6826
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2726..6826
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6827..6874
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6881..6937
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6938..6940,6941..7654)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6938..6940,6941..7651)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7241..7260)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7588..7609
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7669..8257
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7722..7742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8140..8151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8329..8562
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8640..8761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8677..8696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8731..8750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8801..8936
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8956..8975)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8957..8975)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8985..9002)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8985..9001)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8994..9016)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9143..9598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9230..9249)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9440..9461
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9624..9728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9729..9797,9798..10589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9947..9966)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10760..11348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11249..11268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11502..11519
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11636..11657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11672..11677,11678..11695,11696..11697,1..5)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
       61 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
      121 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
      181 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
      241 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
      301 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
      361 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      421 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      481 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
      541 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
      601 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
      661 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
      721 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
      781 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
      841 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
      901 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
      961 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1021 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1081 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1141 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1201 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1261 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1321 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1381 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1441 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1501 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     1561 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     1621 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     1681 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     1741 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     1801 gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga ttccttcata
     1861 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
     1921 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     1981 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     2041 tttcttggct ttatatatct tgtggaaagg acgaaacacc gttgggatgc cggttacaac
     2101 cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     2161 tggcaccgag tcggtgcttt tttgaattct agatcttgag acaaatggca gtattcatcc
     2221 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2281 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2341 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag tggctccggt
     2401 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     2461 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     2521 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     2581 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc
     2641 cgccaccatg gattacaaag acgatgacga taagatggcc ccaaagaaga agcggaaggt
     2701 cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg acatcggcac
     2761 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     2821 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     2881 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     2941 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     3001 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     3061 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     3121 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     3181 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     3241 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     3301 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     3361 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     3421 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     3481 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     3541 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     3601 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     3661 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     3721 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     3781 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     3841 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     3901 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     3961 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     4021 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     4081 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     4141 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     4201 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     4261 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     4321 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     4381 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     4441 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     4501 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     4561 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     4621 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     4681 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     4741 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     4801 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     4861 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     4921 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     4981 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     5041 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     5101 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     5161 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     5221 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     5281 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     5341 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     5401 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     5461 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     5521 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     5581 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     5641 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     5701 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     5761 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     5821 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     5881 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     5941 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     6001 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     6061 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     6121 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     6181 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     6241 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     6301 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     6361 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     6421 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     6481 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     6541 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     6601 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     6661 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     6721 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     6781 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaagc gtcctgctgc
     6841 tactaagaaa gctggtcaag ctaagaaaaa gaaagctagc gccacgaact tctctctgtt
     6901 aaagcaagca ggcgacgtgg aagaaaaccc cggtcccgtg agcaagggcg aggagctgtt
     6961 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     7021 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     7081 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     7141 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     7201 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     7261 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     7321 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     7381 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     7441 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     7501 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     7561 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     7621 gatcactctc ggcatggacg agctgtacaa gtaacgcgtt aagtcgacaa tcaacctctg
     7681 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7741 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7801 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7861 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7921 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7981 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8041 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8101 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8161 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8221 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8281 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8341 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8401 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8461 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8521 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8581 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8641 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8701 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8761 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8821 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8881 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8941 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     9001 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     9061 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9121 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9181 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9241 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9301 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9361 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9421 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9481 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9541 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9781 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9841 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9901 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9961 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10021 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10081 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10141 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10201 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10261 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10321 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10381 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10441 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10501 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10561 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11461 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11521 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11581 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11641 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgta
//
