LOCUS       sequence-363847-        1913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing J23117 anderson promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_044
FEATURES             Location/Qualifiers
     source          1..1913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(615..649)
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     rep_origin      complement(764..1352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(844..863)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1455..1559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1560..1628,1629..1913,1..507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1778..1797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
       61 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      361 gggtcccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
      541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
      601 cttggtctca gccagctagc acaatcccta ggactgagct agctgtcaag gtcagagacc
      661 gactacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      721 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      781 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      841 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      901 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      961 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1021 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1081 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1141 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1201 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1261 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1381 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaca ggtggcactt
     1441 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1501 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1561 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1621 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1681 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1741 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1801 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1861 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta aga
//
