LOCUS       sequence-363848-        8142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transposon containing BiFC VC cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, 
            Ashworth A, Lord CJ
  TITLE     Coupling bimolecular PARylation biosensors with genetic screens to 
            identify PARylation targets.
  JOURNAL   Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4.
  PUBMED    29789535
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-22"; volume: "9(1):2016. doi"; pages: " 
            10.1038/s41467-018-04466-4"
COMMENT     Sequence Label: UPATrap-VC-ORF1
FEATURES             Location/Qualifiers
     source          1..8142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2222..2238
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2222..2238
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2272..2283,2284..2471)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    2511..2544
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2664..2697
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(2756..2789)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2966..2999)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3262..3288
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3391..3642
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3579..3600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3673..3897
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3924..4148
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4816..4849
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5024..5057
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(5103..5136)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             5892..6695
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5955..5974)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6565..6584
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(7034..7067)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    join(7291..7453,7454..7465)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7495..7514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7496..7514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7524..7541)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7524..7540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7533..7555)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7682..8109
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7768..7787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7978..7999
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccagctag ctggcggcgg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tgggcccggc tcgatctccg cggccgcatg atcgattgcc gaagttccta
     2521 ttctctagaa agtataggaa cttcggcatc ggcatcaatt catcatcgga tatttgcatc
     2581 gcaatatcaa tccattgccc ggatggccgg atgatcgatg gcggatgcca tgcgatggat
     2641 cggaaatcgc atccattccg gccgaagttc ctattcttca aatagtatag gaacttcggc
     2701 gaattcggaa tcattggcaa tgccatccat cggatttgat gccatgcaat tccggataac
     2761 ttcgtatagc atacattata cgaagttatc cgaaagctgt ccccaatcct cccaccacga
     2821 cccgccgcac tgcagatacc gcaccgtatt ggcaagtagc ccgtaaacgc ggcgaatcgc
     2881 agccagcata gccaggtcca gccgctcgcc ggggcgctgg cgtttggcca ggcggtcgat
     2941 gtgtctgtcc tccggaaggg cccggataac ttcgtatagt acacattata cgaagttatc
     3001 cgggatctgc tgtatctcta agatggctgg cacgtaaaga gggtctcaat aaatatgcct
     3061 tgaattaatg aaaacagtgg tttctgaatc atggcctcca aagagcattt gccttttccc
     3121 ctctcaccct tctgaaggtg cccaggctgc cctggcagga tgcctctttc tctgtggggt
     3181 ggcattctct gctctctctc taatactctt tctttttctt ccctcctctc ccccaactgc
     3241 aggatgcctt tgtggaactg ctacccatac gatgttccag attacgctct tatggccatg
     3301 gaggcccgaa ttcggtcgac cgagatctct cgaggtaccc gtccggcgtg caaaatcccg
     3361 aacgacctga aacagaaagt catgaaccac gacaagcaga agaacggcat caaggccaac
     3421 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     3481 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     3541 tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3601 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgc cagcacagtg
     3661 gtcgagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3721 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3781 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3841 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3901 ctgaggcgga aagctagact cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3961 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4021 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4081 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4141 ctctatggct tctgaggcgg aaagcttcag cgatggcttc tagctgcccg ggtggcatcc
     4201 ctgtgacccc tccccagtgc ctcttctggc cctggaagtt gccactccag tgcccaccag
     4261 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     4321 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     4381 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     4441 ctccgcctcc tgggttcaag cgattctcct gcctcagcct tctagcttca gcgatggctt
     4501 ctagctgccc gggtggcatc cctgtgaccc ctccccagtg cctcttctgg ccctggaagt
     4561 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     4621 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     4681 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     4741 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     4801 tcccgagtcg agcggataac ttcgtataat gtatgctata cgaagttatc cgcgccaggt
     4861 cgcagatcgt cggtatggag ccgggggtgg tgacgtgggt ctggaccatc ccggaggtaa
     4921 gttgcagcag ggcgtcccgg cagccggcgg gcgattggtc gtaatccagg ataaagacgt
     4981 gcatggaacg gaggcgtttg gccaagacgt ccaaggccca cggataactt cgtataatgt
     5041 gtactatacg aagttatccg atcgatatcc atcccaaata tgcgatggat ggatcggatg
     5101 ccgaagttcc tatactttct agagaatagg aacttcggca ttgcggccgc aaatagatcc
     5161 atccaatgaa gcttgggctg aacatcgagc gccagggctc cgtaaagcta ctagagcaca
     5221 ggcggtgccc caacgtcctg gggcctctcc actaataacg gctacttcca attgattgga
     5281 cgcgccatct tgcctgcctt atgcatattc agcggtgaac tgaatattca tgaacgaggc
     5341 ccgtcccgtc cctccctcct tccccccacc cccggaaccc gctccggagg acccgaaggg
     5401 ccccgccttc attaccgatg cgtaggacaa accattttcc cgatgtgtgt ggggggatac
     5461 taatgagaga ctttagctga aaatgagcct gaactccgaa gctgagtaaa aatggcctaa
     5521 ctttatcctc cgttctgtaa gtcctcggtt tgagtgcacg ggaaacccga aaggaggacg
     5581 acaggaccag gaacattctc ctcctcctgt cgcgtcagaa agaacaccca accagggagc
     5641 cggagcccta gcgtcaacaa ctccgccgcg cgcgctccgt gtaggccggt gcgggcggcc
     5701 ccgtagcgca agggagggcg ggaaaggaag gggcgggaca caagggcgaa tctataaagg
     5761 gcgtcactca gccagttctc tcctcagaag cgccgagagc gcgaccggga cggttggaga
     5821 agaaggtggc tcccggaagg gggagagaca aactgccgta acctctgccg ttcaggatcc
     5881 gtgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     5941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6061 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6421 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     6481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6661 tcgccttcta tcgccttctt gacgagttct tctgaccacc ggtgacgcat ggccaatcgc
     6721 atcatggatt ccatccgatg cgaatcgcat ccatggatgc gccaatcgga ttccatgcga
     6781 tgccatttcc gatgcgggat ccgaaattcc agacaagttt gttgttggat atgccctcga
     6841 ctacaacgag tacttcagga atttgcaggt aagtaatgct tttttttttc tcgctctcat
     6901 ttttcaaaaa cccacataaa aattgaggaa agggaagaat tgttttctcc ttccagcacc
     6961 tcgtaatttg acccgactga tggttcccat tagtcacata aagctgtagt caagtacaga
     7021 cgtcgaattc gccgaagttc ctatactatt tgaagaatag gaacttcggc ctcgagcatg
     7081 catcatatcc atcgcaatcg catccccatt caattcacat cgcatatccg atgccgatat
     7141 ggcatcggca ttgccatcca atcctgatca atccatatat gatgatatat atatgatgat
     7201 atatatatat ttgggcattg cgcgatgatc aatcaaaatc catccgaatg gatccatgcg
     7261 gatcatcgca tccattgcga taaaggatct ttcttgcttt tacttttact tccttaatac
     7321 tcaagtacaa ttttaatgga gtactttttt acttttactc aagtaagatt ctagccagat
     7381 acttttactt ttaattgagt aaaattttcc ctaagtactt gtactttcac ttgagtaaaa
     7441 tttttgagta ctttttacac ctctgtcaag aaccatatgc cggtacccaa ttcgccctat
     7501 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7561 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7621 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7681 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7741 accacacccg ccgcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7801 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7861 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7921 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7981 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8041 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8101 acgcgaattt taacaaaata ttaacgctta caatttaggt gg
//
