LOCUS       sequence-363921-       14296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT9_i1 fused to GFP10 and of 
            human SEPT7 fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 14296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10
FEATURES             Location/Qualifiers
     source          1..14296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    22..103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1661..2048
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1742..1760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1778..1796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1813..1831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1849..1867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1885..1903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1920..1938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1956..1974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(4121..4138)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4348..4367
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4529..5230
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5513..5693
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7221..7240)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7454..7473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7573..7595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7633..7651)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7719..7823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7824..7892,7893..8684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8042..8061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8855..9443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9344..9363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9597..9614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9684..9704)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9689..10018
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9869..10004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11152..11217
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11347..11367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11792..11926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11883..11902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12094..12727
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12774..12899
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13392..13625
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13810..13854
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14147..14264
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttggctgata cgcgtacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtatggctg attatgatcc tctagattaa aagatcttcc ctttcttctt
      241 gttcttttcc aaggttcttg aagagttctg ttgttctaaa atacgttgtt gagcttccca
      301 gtttgctttc tcatcctcga actgacgacg tttttcctcc aattctttgt gctgtgcttc
      361 caaattcttt ttcatttgct catggcgccg ctggagctca gcttcagagt ccttcagttt
      421 ttgaactttt tctttgacct tcatctcaaa cacctgctcc atctccatct ccatcttctt
      481 cattttagct acatgctccc ttctttcttc ttccatttgt gccagagggc tcttagtcag
      541 ctgcccttta ttcttgttgt tatcaactcc attataagtc acagctgcaa gttttctgct
      601 tctgtagttc tcatagtgga cattattagt aacatctttc aagtcctgca tgtgtgttct
      661 tatcaacata tttcttagga ttgtaaaatc acaatgttca ccattttcaa cttcagcaac
      721 accccaagga tactgccttc ctctgaccct tttgccatta acttcaatga tagtattact
      781 acctaccaca gcaagaggta aacggtcctt tatcttttta acaagtttat tttcttcttc
      841 atcatctgtt tctggaaatt cgtatatttt aattttatgt tcttggattt ctttcattat
      901 ctgtttttta aactgttggc attcctctgg tgtgagtgtg tctgctttgg caataagtgg
      961 gatgatattc actttttcat gcaaacgctt cataaactca atatccaatg gtttaagtcc
     1021 atgtcctgaa ggagcaatga agtataaaca acactgcacc ctgttatcag gcatctgacg
     1081 tctgttcact cgtgattctg catttaggta gtcctcaaat ttactatcaa tgtagtcgat
     1141 aacaggctgc cagcaattac tattatccac tgcatctcca aatcctgggg tatcaactat
     1201 tgtgagcagc aactgaacac caccttcttt gattaaaact ttggattgtt ccacctgtac
     1261 agtcttttta attctatgag aaggacctgg atactctgga gaatacaaat ctgtgaggaa
     1321 taatgagttg attaatgtcg actttcccaa tccagattca cccactacca taagcgtgaa
     1381 ttcaaaacct ctcttcaccg attttctgta tacttgattt gggagattgg caaatcccac
     1441 atagccttca aggttcttct gttgagctac catggtgctg ctgttgacgc tcctctcctc
     1501 agcagcagcg gatctcgcac tgaccgacat tccggaggac ccaccacctc cagagccacc
     1561 gccaccatcg atggaggcgt cggtgatgcc ggcggcggtc acgtactcca gcagcaccat
     1621 gtggtcgcgc ttctccatca tatgaccggt gaattctcca ggcgatctga cggttcacta
     1681 aacgagctct gcttatatag gcctcccacc gtacacgcca cctcgacata ctcgagttta
     1741 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
     1801 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1861 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
     1921 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     1981 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2041 agatcgcctg gagatttcga gctcggtacc gcggatgaag aagtcttact caggaggcac
     2101 gcggacctcc agtggccggc tccggaggct tggtgactcc agtggcccag ccttaaaaag
     2161 atcttttgag gtcgaggagg tcgagacacc caactccacc ccaccccgga gggtccagac
     2221 tcccctactc cgagccactg tggccagctc cacccagaaa ttccaggacc tgggcgtgaa
     2281 gaactcagaa ccctcggccc gccatgtgga ctccctaagc caacgctccc ccaaggcgtc
     2341 cctgcggagg gtggagctct cgggccccaa ggcggccgag ccggtgtccc ggcgcactga
     2401 gctgtccatt gacatctcgt ccaagcaggt ggagaacgcc ggggccatcg gcccgtcccg
     2461 gttcgggctc aagagggccg aggtgttggg ccacaagacg ccagaaccgg cccctcggag
     2521 gacggagatc accatcgtca aaccccagga gtcagcccac cggaggatgg agccccctgc
     2581 ctccaaggtc cccgaggtgc ccactgcccc tgccaccgac gcagccccca agagggtgga
     2641 gatccagatg cccaagcctg ctgaggcgcc caccgccccc agcccagccc agaccttgga
     2701 gaattcagag cctgcccctg tgtctcagct gcagagcagg ctggagccca agccccagcc
     2761 ccctgtggct gaggctacac cccggagcca ggaggccact gaggcggctc ccagctgcgt
     2821 tggcgacatg gccgacaccc ccagagatgc cgggctcaag caggcgcctg catcacggaa
     2881 cgagaaggcc ccggtggact tcggctacgt ggggattgac tccatcctgg agcagatgcg
     2941 ccggaaggcc atgaagcagg gcttcgagtt caacatcatg gtggtcgggc agagcggctt
     3001 gggtaaatcc accttaatca acaccctctt caaatccaaa atcagccgga agtcggtgca
     3061 gcccacctca gaggagcgca tccccaagac catcgagatc aagtccatca cgcacgatat
     3121 tgaggagaaa ggcgtccgga tgaagctgac agtgattgac acaccagggt tcggggacca
     3181 catcaacaac gagaactgct ggcagcccat catgaagttc atcaatgacc agtacgagaa
     3241 atacctgcag gaggaggtca acatcaaccg caagaagcgc atcccggaca cccgcgtcca
     3301 ctgctgcctc tacttcatcc ccgccaccgg ccactccctc aggcccctgg acatcgagtt
     3361 tatgaaacgc ctgagcaagg tggtcaacat cgtccctgtc atcgccaagg cggacacact
     3421 caccctggag gagagggtcc acttcaaaca gcggatcacc gcagacctgc tgtccaacgg
     3481 catcgacgtg tacccccaga aggaatttga tgaggactcg gaggaccggc tggtgaacga
     3541 gaagttccgg gagatgatcc catttgctgt ggtgggcagt gaccacgagt accaggtcaa
     3601 cggcaagagg atccttggga ggaagaccaa gtggggtacc atcgaagttg aaaacaccac
     3661 acactgtgag tttgcctacc tgcgggacct tctcatcagg acgcacatgc agaacatcaa
     3721 ggacatcacc agcagcatcc acttcgaggc gtaccgtgtg aagcgcctca acgagggcag
     3781 cagcgccatg gccaacggcg tggaggagaa ggagccagaa gccccagaga tgatcgatgg
     3841 tggcggtggc tctggaggtg gtgggtcctc cggaatgggc gacctgcccg acgaccacta
     3901 cctgtccacc cagaccatcc tgagcaagga cctgaactaa gctagctaat agttccgccc
     3961 ctctccctcc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     4021 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     4081 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     4141 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     4201 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     4261 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     4321 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     4381 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     4441 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     4501 gttttccttt gaaaaacacg atgcaagcat gagcgagctg attaaggaga acatgcacat
     4561 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     4621 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     4681 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     4741 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     4801 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     4861 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     4921 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     4981 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     5041 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     5101 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     5161 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     5221 gcttaattaa ggatccagcg gccgctcgag acctagaaaa acatggagca atcacaagta
     5281 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5341 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5401 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5461 gacaaaatcg tcgagagatg ctgcatataa gcagctgctt tttgcttgta ctgggtctct
     5521 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5581 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5641 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     5701 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     5761 gaggccttga cattataata gatttagcag gaattgaact aggagtggag cacacaggca
     5821 aagctgcaga agtacttgga agaagccacc agagatactc acgattctgc acatacctgg
     5881 ctaatcccag atcctaagga ttacattaag tttactaaca tttatataat gatttatagt
     5941 ttaaagtata aacttatcta atttactatt ctgacagata ttaattaatc ctcaaatatc
     6001 ataagagatg attactatta tccccattta acacaagagg aaactgagag ggaaagatgt
     6061 tgaagtaatt ttcccacaat tacagcatcc gttagttacg actctatgat cttctgacac
     6121 aaattccatt tactcctcac cctatgactc agtcgaatat atcaaagtta tggacattat
     6181 gctaagtaac aaattaccct tttatatagt aaatactgag tagattgaga gaagaaattg
     6241 tttgcaaacc tgaatagctt caagaagaag agaagtgagg ataagaataa cagttgtcat
     6301 ttaacaagtt ttaacaagta acttggttag aaagggattc aaatgcataa agcaagggat
     6361 aaatttttct ggcaacaaga ctatacaata taaccttaaa tatgacttca aataattgtt
     6421 ggaacttgat aaaactaatt aaatattatt gaagattatc aatattataa atgtaattta
     6481 cttttaaaaa gggaacatag aaatgtgtat cattagagta gaaaacaatc cttattatca
     6541 caatttgtca aaacaagttt gttattaaca caagtagaat actgcattca attaagttga
     6601 ctgcagattt tgtgttttgt taaaattaga aagagataac aacaatttga attattgaaa
     6661 gtaacatgta aatagttcta catacgttct tttgacatct tgttcaatca ttgatcgaag
     6721 ttctttatct tggaagaatt tgttccaaag actctgaaat aaggaaaaca atctattata
     6781 tagtctcaca cctttgtttt acttttagtg atttcaattt aataatgtaa atggttaaaa
     6841 tttattcttc tctgagatca tttcacattg cagatagaaa acctgagact ggggtaattt
     6901 ttattaaaat ctaatttaat ctcagaaaca catctttatt ctaacatcaa tttttccagt
     6961 ttgatattat catataaagt cagccttcct catctgcagg ttccacaaca aaaatccaac
     7021 caactgtgga tcaaaaatat tgggaaaaaa ttaaaaatag caatacaaca ataaaaaaat
     7081 acaaatcaga aaaacagcac agtataacaa ctttatttag catttacaat ctattaggta
     7141 ttataagtaa tctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     7201 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     7261 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     7321 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     7381 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     7441 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     7501 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     7561 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7621 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     7681 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7801 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7861 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7921 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7981 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8041 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8101 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8161 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8221 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8281 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8341 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8401 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8461 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8521 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8581 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8641 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8701 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8761 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8821 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8941 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9001 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9061 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9241 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9481 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9541 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9601 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9661 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     9721 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     9781 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9841 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9901 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     9961 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10021 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
    10081 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
    10141 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
    10201 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
    10261 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
    10321 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
    10381 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
    10441 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
    10501 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
    10561 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
    10621 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
    10681 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
    10741 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
    10801 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
    10861 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
    10921 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
    10981 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
    11041 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
    11101 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
    11161 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
    11221 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
    11281 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
    11341 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
    11401 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
    11461 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
    11521 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
    11581 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    11641 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    11701 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    11761 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    11821 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    11881 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    11941 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    12001 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    12061 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
    12121 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
    12181 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
    12241 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
    12301 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
    12361 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
    12421 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
    12481 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
    12541 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    12601 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    12661 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    12721 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    12781 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    12841 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    12901 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    12961 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    13021 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    13081 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    13141 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    13201 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
    13261 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    13321 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    13381 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    13441 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    13501 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    13561 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    13621 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    13681 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    13741 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    13801 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    13861 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    13921 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    13981 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    14041 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    14101 tctcgacggt atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag
    14161 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    14221 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    14281 acagcagaga tccact
//
