LOCUS       sequence-363937-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent
            transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Rampon C, Gauron C, Lin T, Meda F, Dupont E, Cosson A, Ipendey E, 
            Frerot A, Aujard I, Le Saux T, Bensimon D, Jullien L, Volovitch M, 
            Vriz S, Joliot A
  TITLE     Control of brain patterning by Engrailed paracrine transfer: a new 
            function of the Pbx interaction domain.
  JOURNAL   Development. 2015 May 15;142(10):1840-9. doi: 10.1242/dev.114181. 
            Epub 2015 Apr 29.
  PUBMED    25926358
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.114181"; journalName: "Development"; date: "2015-05-15-
            15"; volume: "142"; issue: "10"; pages: "1840-9"
COMMENT     Sequence Label: pT22Ubm5Eng2aE4PCh(#283)
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(152..172)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(152..170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(181..192,193..380)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    399..520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(540..1247,1248..1250,1251..1253))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(579..600)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     928..947
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1187..1208
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2014..2025)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5677..5706
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5716..5745
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5755..5784
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5794..5823
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5833..5862
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6703..6723
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7690..7746
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7756..8466
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8494..8615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8711..8848,8849..8860)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8928..8945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9099..9687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9179..9198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9858..10649,10650..10718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10481..10500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10719..10823)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc ccgatactcc agcttttgtt ccctttagtg agggttaatt gcgcgttaca
      181 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
      241 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
      301 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
      361 ttattttttg gagatcactt cctcaggacc agacatgata agatacattg atgagtttgg
      421 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      481 tgctttattt gtaaccatta taagctgcaa taaacaagtt gcgtgcgcat ttatttaagg
      541 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      601 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      661 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      721 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc
      781 tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag cttgtgcccc
      841 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      901 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      961 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     1021 tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac gagggtgggc
     1081 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     1141 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     1201 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgtcggct
     1261 ggcgaccggt ggatcatccc ccgcaagtct gactgagtgg aagtttcagt tcaatggaag
     1321 ggagaaaaga gccagccttt atatatgtca tgtattgctg ctgtctgcat gcgatgaacc
     1381 tagagcttac tttttctgtc aagtaaggca aaagcagaga acctttgtct tgcattgtgt
     1441 ttgatgttgt gctgtgacag tcatttctta gttcatgctg gtacttttgt tccatagctt
     1501 tacccaaaga gttatccagc attcccagtt attccaaaaa ctcaatgtca caaattttcc
     1561 agtccattgc ttagcagcgt tgaccccgga gaccctgatg tggtaattta cagtgcaatt
     1621 cagttgctgg tccaaacact ttgtaacagt ttaaagaatt gtggttacta gtaaatcctt
     1681 aatttggcat catctaggtg tcattctttg ccacttttga atcacacaaa tgaaccaaac
     1741 aatggttagc tagcatcaaa catttaccaa ataatctcat cagtgtcacc ccaatgttta
     1801 agaagccaca ttttatgtcc ccagatatat taatattatt ttatatataa tttaaaatag
     1861 taatgtggtg ttactgtatt tgtcatataa cgcaatatat acagtatgtc tttatctgta
     1921 tcaattttgt ggtcacacca tcaataaact ccagtttaat tatttaaaat tgggtagtga
     1981 taaccatata aaggaacagt ggctagttcc atgttgtagc tcccaacctg ctttcaggtg
     2041 atccatgcag tgtcgtataa aaaggtaaca atttttaacc ttggaaagca agtacactta
     2101 aggtcccaga tctgaagcta cccctaacac actattctac acacagaaaa tgctcttcac
     2161 taggtcgaga ccagcaaagt tctagaattt gtcgaaacat ttatgttata tatttcctga
     2221 aaaaaattct gagtaagttc ttaagtgtta ttgccagcaa cataaacaac agacggcaaa
     2281 atgaataaat gataacaaag cagtaggctt aaataaacct aatttttata ggctgttctc
     2341 tacaaccctc aaacagtgat tagttttgta cttataaact tgccctttca ttcatatttc
     2401 aagaaaattg gttcagaaga tctggatatt ctagcagttg ttcaagctca tggagggatc
     2461 agtgacctga ttccacaatg actaggccta atccagaaat tagatgactg tcaacataaa
     2521 aaggcacagc actcactagc tgccctatat attttattat attttacata tattatttta
     2581 tttatttagc tctgagtgct gtactttctg gttaaagaaa actgcttaca acagctaacc
     2641 tgtactacct caggctcagg gaatttggaa caggtttgtc tggtttgttt ctttaaccat
     2701 gcatgcttgt tttcaactat ggcaacacag tcacatggga cattacagaa atgatttgtc
     2761 gatgacatgc gacttttctt taataaagcg caaagatccc aaaaagcaaa cttttaacaa
     2821 aaatcatata attatatttt caatccagct ttgtagcaac tttgtgctgc tgttcactca
     2881 gcaacagata gtcagtataa ggtcagtgtg tctcaaagca gtgccatctg tttcacacat
     2941 tgcgttctat atataagtgt gctggttgac acgacactgt ataaggccta ggctaaaaca
     3001 caaacaatgt agaatgacac tgtgtttttt ttgtaaacaa atgttgtttt tggttaaaca
     3061 tctttgtgaa aacatcctcc tgtcatgtat ttgctatatt caaatgttaa acccgtgcag
     3121 aatagaacat atacaaaaaa aaacaacaca acacattttt aaacattatt aaatatcaag
     3181 tattgctggc agttctgttt ctgttttaca gtaccctttg ccacagttct ccgcttttcc
     3241 tggtccagat tccacaagtc tgattcacca atagcaaagc gaataaacaa ccaaagcagc
     3301 caatcactgc ttgtagactg tcctgcgaga ccggcccatt ccagcacatt ctggaaactt
     3361 cctttatatg ataattataa atacatttaa attattgata caaaacatgt aattcctaga
     3421 acataaccat agcaatcatt agttttcagg gtaattatgt atttttagga tttgactgcg
     3481 gaaagatctg gtcatgtgac gtctcatgaa cgtcacggcc ctgggtttct ataaatacag
     3541 taggactctc gaccatcggc agatttttcg aagaagaaga tcagtttcag gagccgtact
     3601 gttccgtttc aacgcaaata ttaacggtaa gagcgaattt cctagtttgt tttcatgcca
     3661 ttctttaaaa ccatagcgta ttactttaat tatagtaaac tttcgctttc tttattacaa
     3721 gagacgtttt gtgttgattc tccgcggaca ttttcggtca gacaatcaga aaatgaccgc
     3781 ggaggaccag taacttgcat tacacgtaag ttaaatcttc gtgtattaaa atggttaggt
     3841 tgttaacgtc aaataggtta ccgtgtttgc gtgtgatcag gttggttttg ttagattttt
     3901 gtcagtattt ttaatttatt tgttttagtt tatttatttt ttttgctgaa tcatagtttg
     3961 tgaacaaaga acccggatgt tacatacagt acagccgcca tgttacagag agttataact
     4021 taatcatttt aaaaataatt ttgccttact tttagtttgt catgttgaga aatgaggaaa
     4081 tgttaaaatg aggaaatatc caattaattt aatatatcaa aataatccat gattacaatg
     4141 cactgaactg gagaaaatta agatgttttc tagtgtcatg aaacaaatgt aagagatgta
     4201 cattgtagat gttttatgtc aagaattggc tagttgatgc agcatactgg cgatactcag
     4261 ttgtaataac agtaacgtta catgttaata gactactgag tatgctgttc tgtctatgta
     4321 tgctctgtaa gctacgagaa ggactttttt aaacagtaaa gggtgcaata tttttacaaa
     4381 ttgaattaaa taaaggctgt ctattaagta atatgcttga tatttttctt acttgatcgg
     4441 aaataagaaa aaatataaac gttgttgctc taaaaatcct agttcagttt agccaaccac
     4501 aaataccttt ttgttcctcc aacagttttt ttttcttctc tataatattt ggcagtctat
     4561 agtactccaa atgtttcccc acagtctaac taattggtac agccaaaatc atgacactta
     4621 ttgcaataat aattttggtt cattggcatt gttgatagcc tgtgccacta atatggtcga
     4681 ttgatcatgc ttcaggaaga aaactatatt gtttgatgta agattattaa atcttcacct
     4741 gcttccatta caaactattc ccatcttatt gaattctggt atgtcttaaa ggattagttc
     4801 acttcccaaa tcaaaattta cttagccttt tttcatccat gatccctttt tttcatcatt
     4861 aatgaagaaa ttgtttttga aaaagtttca agattttttt ctctatattg tggagcttgt
     4921 tagtttaaaa ttccaaaatg caatatgtgg cttcaaatgg ttctaaatga tcccagtcaa
     4981 ggaataacag tcttatctaa tgaaaccatt agacctttaa aaataaaaaa aataaaagta
     5041 tttattttta aatgactgag tgattaagtt gaatttcagc gtttccttac tgtgtagaag
     5101 tccttcctta ctggccccac cctttggttc tctgccaatc tgctacctaa tgtaatgttg
     5161 tggaacatta ttattcttta tttcttaatt ttttattttt tattttaaaa caatgtaaac
     5221 tgcacagatg tgcagtttgt ttaaaatggc caatgctttg gaaatgcatg acataattag
     5281 atttcatgat gcacaaagcc aaatctcaga gcttgtgcaa aatgagctat catttcacta
     5341 ggtaagaccc taaattttca tataggatca tttggacaat tttgctgcag gtaaaatgca
     5401 ttctatagtc cactgtcagc cattgttttg gatagtattt atttttctct acaagtatag
     5461 tcaatagttt tctattattt taaaggtttg taacatttaa gggtgaccaa atgcaaagta
     5521 aaatttcatt ttcgggtgaa ctatctcgtt taacatggga gaagtgcaaa acatacatta
     5581 ttggctagaa cattgtagta ttttttaaat ggaaatgtgt gattgctaat cttactttga
     5641 atttgtttac agggatccca tcgatttaaa gctatggagc aaaagctcat ttctgaagag
     5701 gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa
     5761 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac
     5821 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagagcttg
     5881 ggcgacctca ccatggatga gaatgagcag agcgcaagag atgtggaaca gcgcggagcg
     5941 tcggacgagt ccaacagtgc catacgaccc cttttacaag ctcctggaaa cctccaactg
     6001 ccacaccgaa tcacaaactt cttcatcgac aacatcctgc gaccagattt tggccgtaaa
     6061 aaagaagcaa atatcacgcg ctatgaggac aatcacggcg cacgagagaa tcacaaccct
     6121 acgggtccaa gcacgggaca ggtgggaagc actgtaccag cggaagaagc ctccacaacg
     6181 catacgagca gcggaggaaa ggaggcagag atagagagcg aagaacccct gaagcctcgc
     6241 ggggagaatg tggatcagtg cttgggttca gaatcggata gttcacagag caattcaaac
     6301 ggacagactg gtcagggtat gctgtggccc gcttgggttt actgcacacg ctactcggac
     6361 aggccgtcgt ctggtcccag gtctcgtaaa ccaaagaaga aagccgcaag taaagaagac
     6421 aaacgaccac gcacggcctt cacggcggag cagcttcaga gactcaaggc cgagttccag
     6481 accaaccgct acctgaccga gcagcggcgg caaagcctgg cgcaggaact gggcctcaac
     6541 gaatctcaga tcaaaatctg gttccaaaac aagcgggcca aaatcaaaaa ggccagcgga
     6601 gtcaagaacg gtctggcaat acacctgatg gcacagggac tgtataacca cagcaccaca
     6661 tcaaaggagg acaaatcaga cagtcacgga ctgcccgggc cagagaacct gtacttccag
     6721 gggctcgagc cctctgccgg agacatgaga gctgcaaacc tttggccgag cccgctgatg
     6781 attaaacgct ctaagaagaa cagcctggcc ttgtccctga ccgccgacca gatggtcagt
     6841 gccttgttgg atgccgagcc ccccatactc tattccgagt atgatcctac cagacccttc
     6901 agtgaggctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
     6961 attaactggg ccaagagggt gccaggcttt gtggatttga ccctccacga tcaggtccac
     7021 ctcctagaat gtgcctggct agagatcctg atgattggtc tcgtctggcg cagcatggag
     7081 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
     7141 gtagagggca tggtggagat tttcgacatg ctgctggcta catcatctcg gttccgcatg
     7201 atgaatctgc agggagagga atttgtgtgc ctcaaatcta ttattttgct taattctgga
     7261 gtttacacat ttctgtccag caccctgaag tctctggagg aaaaggacca tatccatcga
     7321 gtgctggaca agatcacaga cactttgatt cacctgatgg ctaaggctgg cctgaccctg
     7381 cagcagcagc atcagagact cgcacagctt ctcctgatcc tctcccacat caggcacatg
     7441 agtaacaaag gcatggagca tctgtactct atgaagtgca agaacgtggt gcccctctat
     7501 gatctgctgc tggaggcggc ggacgctcac cgcctacatg cgcccactag ccgtggaggg
     7561 gcatccgtgg aggaaacaga ccaaagccac ttggctactg cgggctctac ttcatctcat
     7621 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agcaccaggt
     7681 tccggcagcg ctacaaactt ctctctgctc aagcaggctg gagatgtgga agaaaaccct
     7741 gggcccgaag acggcatggt gagcaagggc gaggaggata acatggctat catcaaggag
     7801 ttcatgcgct tcaaggttca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     7861 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgacaaag
     7921 ggcggccccc tgcccttcgc ctgggacatc ctgagccctc agttcatgta cggctccaaa
     7981 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcttt cccagagggc
     8041 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgacagt gacccaggac
     8101 tcttctctgc aggacggcga gtttatctac aaggtgaagc tgcgcggcac caacttcccc
     8161 tccgacggcc ccgtaatgca gaagaaaact atgggatggg aggcttcttc cgagcggatg
     8221 tacccagagg acggagccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     8281 ggccactacg atgccgaggt caagaccacc tacaaggcca agaagcccgt gcaactgccc
     8341 ggagcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     8401 gtggaacagt acgaacgcgc cgagggccgc cacagcaccg gcggcatgga cgagctgtac
     8461 aagtaaggcg cgccgatatc tacgtagaca tgataagata cattgatgag tttggacaaa
     8521 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     8581 tatttgtaac catattaagc tgcaataaac aagttaacaa caacaattgc attcatttta
     8641 tgtttcaggt tcagggggag gtgtgggagg ttttttcctg caggactcta gtcgcggccg
     8701 ctcttccgct aatactcaag tacaatttta atggagtact tttttacttt tactcaagta
     8761 agattctagc cagatacttt tacttttaat tgagtaaaat tttccctaag tacttgtact
     8821 ttcacttgag taaaattttt gagtactttt tacacctctg ccctgcatta atgaatcggc
     8881 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8941 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9001 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9061 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10141 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10201 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10261 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10321 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10381 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10441 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10501 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10561 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10621 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10681 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10801 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
