LOCUS       sequence-363947-        7857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP1-10 and GFP11×11-GFP-tDeg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Zhou WJ, Li H, Zhang KK, Wang F, Chu X, Jiang JH
  TITLE     Genetically Encoded Sensor Enables Endogenous RNA Imaging with 
            Conformation-Switching Induced Fluorogenic Proteins.
  JOURNAL   J Am Chem Soc. 2021 Sep 8;143(35):14394-14401. doi: 
            10.1021/jacs.1c07719. Epub 2021 Aug 25.
  PUBMED    34431301
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.1c07719"; journalName: "J Am Chem Soc"; date: 
            "2021-09-8- 8"; volume: "143"; issue: "35"; pages: "14394-14401"
COMMENT     Sequence Label: GFP1-10-miniCMV-GFP11×11-GFP-tDeg
FEATURES             Location/Qualifiers
     source          1..7857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..1548
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     primer_bind     complement(1684..1701)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1690..1914
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1928..1966
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1963..1987
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2002..2011
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2014..2061
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             2077..2124
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             2140..2187
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             2203..2250
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             2284..2709
                     /codon_start=1
                     /product="seven tandem copies of the 11th beta-strand of 
                     superfolder GFP"
                     /label=GFP11x7
                     /note="each GFP11 associates with GFP(1-10), consisting of 
                     the first 10 beta-strands, to reconstitute fluorescent GFP 
                     (Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDH
                     MVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSG
                     GRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGIT"
     CDS             2740..3381
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     CDS             3382..3429
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     polyA_signal    3557..3691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      3724..4152
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3811..3830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4021..4042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4161..4181)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4166..4495
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4346..4481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4408..4427
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4562..5356
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4616..4635)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5226..5245
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5530..5651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5700..5716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5700..5716)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5713..5735)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5724..5740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5748..5754,5755..5772,5773..5778))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5793..5814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5931..5948)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6102..6690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6182..6201)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6861..7652,7653..7721))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7484..7503
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7722..7826)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgt ccaaaggaga agaactgttt accggtgttg tgccaatttt ggttgaactc
      961 gatggtgatg tcaacggaca taagttctca gtgagaggcg aaggagaagg tgacgccacc
     1021 attggaaaat tgactcttaa attcatctgt actactggta aacttcctgt accatggccg
     1081 actctcgtaa caacgcttac gtacggagtt cagtgctttt cgagataccc agaccatatg
     1141 aaaagacatg acttttttaa gtcggctatg cctgaaggtt acgtgcaaga aagaacaatt
     1201 tcgttcaaag atgatggaaa atataaaact agagcagttg ttaaatttga aggagatact
     1261 ttggttaacc gcattgaact gaaaggaaca gattttaaag aagatggtaa tattcttgga
     1321 cacaaactcg aatacaattt taatagtcat aacgtataca tcactgctga taagcaaaag
     1381 aacggaatta aagcgaattt cacagtacgc cataatgtag aagatggcag tgttcaactt
     1441 gccgaccatt accaacaaaa cacccctatt ggagacggtc cggtacttct tcctgataat
     1501 cactacctct caacacaaac agtcctgagc aaagatccaa atgaaaaagg aacaggtggc
     1561 ggcggaagtt agaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct
     1621 gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat
     1681 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1741 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     1801 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     1861 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     1921 aggcggaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg
     1981 tcagatcgcg ccgacatcac cgccaccatg gcgcgggacc acatggtgct gcatgaatac
     2041 gtgaacgcgg cggggataac gggtgggtcc ggaggtagag accatatggt tctccacgag
     2101 tacgtcaacg cagcgggcat tacaggcggt tcaggtggca gggatcacat ggtgttacat
     2161 gaatatgtaa atgcggctgg aatcaccgga ggatctggag gccgggacca tatggtcttg
     2221 cacgagtacg ttaacgctgc tggtataacc gggggctcgg gcggtggtag tgggagcaac
     2281 atgcgtgacc acatggtcct tcatgagtat gtaaatgctg ctgggattac aggtggctct
     2341 ggaggtagag atcatatggt tctccacgaa tacgttaacg ccgcaggcat cactggcggt
     2401 agtggaggac gcgaccatat ggtactacat gaatatgtca atgcagccgg aataaccgga
     2461 gggtccggag gccgggatca catggtgctg catgagtatg tgaacgcggc gggtataact
     2521 ggtgggtcgg gcggacgaga ccatatggtg cttcacgaat acgtaaacgc agctggcatt
     2581 actggcggat caggtggcag ggatcacatg gtactccatg agtacgtgaa cgctgctgga
     2641 atcacaggcg gtagcggcgg tcgggaccat atggtcctgc acgaatatgt caatgctgcc
     2701 ggtatcaccg gcggtagcgg cggtggcggt agcggcggta tgtccaaagg agaagaactg
     2761 tttaccggtg ttgtgccaat tttggttgaa ctcgatggtg atgtcaacgg acataagttc
     2821 tcagtgagag gcgaaggaga aggtgacgcc accattggaa aattgactct taaattcatc
     2881 tgtactactg gtaaacttcc tgtaccatgg ccgactctcg taacaacgct tacgtacgga
     2941 gttcagtgct tttcgagata cccagaccat atgaaaagac atgacttttt taagtcggct
     3001 atgcctgaag gttacgtgca agaaagaaca atttcgttca aagatgatgg aaaatataaa
     3061 actagagcag ttgttaaatt tgaaggagat actttggtta accgcattga actgaaagga
     3121 acagatttta aagaagatgg taatattctt ggacacaaac tcgaatacaa ttttaatagt
     3181 cataacgtat acatcactgc tgataagcaa aagaacggaa ttaaagcgaa tttcacagta
     3241 cgccataatg tagaagatgg cagtgttcaa cttgccgacc attaccaaca aaacacccct
     3301 attggagacg gtccggtact tcttcctgat aatcactacc tctcaacaca aacagtcctg
     3361 agcaaagatc caaatgaaaa acgtgaccac atggtccttc atgagtatgt aaatgctgct
     3421 gggattacac acgggatgga cgagctgtac aagggcggaa gatccggtgg tggttctggt
     3481 cctcgtcccc gtggtactcg tggtaaaggt cgccgtattc gtcgccgcgg ttagaatgaa
     3541 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3601 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3661 actcatcaat gtatcttatc atgtctggat caagaaccag ctggggctct agggggtatc
     3721 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3901 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3961 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4021 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4081 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4141 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4201 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4261 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4321 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4381 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4441 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4501 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4561 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4621 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4681 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4741 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4801 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4861 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4921 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4981 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5041 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5101 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5161 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5221 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5281 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5341 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5401 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5461 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5521 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5581 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5641 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5701 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5761 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5821 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5881 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5941 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6001 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6061 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6121 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6181 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6241 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6301 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6361 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6421 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6481 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6541 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6601 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6661 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6721 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6781 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6841 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6901 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6961 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7021 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7081 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7141 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7201 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7261 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7321 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7381 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7441 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7501 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7561 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7621 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7681 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7741 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7801 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
