LOCUS       sequence-363962-       11431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible Catalase activation in zebrafish permanent 
            transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11431)
  TITLE     Vriz lab cyclofen-inducible ERT2 plasmids
REFERENCE   2  (bases 1 to 11431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT22U6CatalE4PCh(#767)
FEATURES             Location/Qualifiers
     source          1..11431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    397..518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(538..1245,1246..1248,1249..1251))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(577..598)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     926..945
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1185..1206
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2012..2023)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        5658..5676
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5658..5675
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             7286..7306
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             8273..8329
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8339..9049
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    9077..9198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9294..9431,9432..9443)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9511..9528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9682..10270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9762..9781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10441..11232,11233..11301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11064..11083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11302..11406)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcaggaccag acatgataag atacattgat gagtttggac
      421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      481 ctttatttgt aaccattata agctgcaata aacaagttgc gtgcgcattt atttaaggta
      541 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      601 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      661 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      721 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      781 cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct tgtgccccag
      841 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      901 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      961 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1021 gtcggggtag cggctgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
     1081 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1141 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1201 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgtcggctgg
     1261 cgaccggtgg atcatccccc gcaagtctga ctgagtggaa gtttcagttc aatggaaggg
     1321 agaaaagagc cagcctttat atatgtcatg tattgctgct gtctgcatgc gatgaaccta
     1381 gagcttactt tttctgtcaa gtaaggcaaa agcagagaac ctttgtcttg cattgtgttt
     1441 gatgttgtgc tgtgacagtc atttcttagt tcatgctggt acttttgttc catagcttta
     1501 cccaaagagt tatccagcat tcccagttat tccaaaaact caatgtcaca aattttccag
     1561 tccattgctt agcagcgttg accccggaga ccctgatgtg gtaatttaca gtgcaattca
     1621 gttgctggtc caaacacttt gtaacagttt aaagaattgt ggttactagt aaatccttaa
     1681 tttggcatca tctaggtgtc attctttgcc acttttgaat cacacaaatg aaccaaacaa
     1741 tggttagcta gcatcaaaca tttaccaaat aatctcatca gtgtcacccc aatgtttaag
     1801 aagccacatt ttatgtcccc agatatatta atattatttt atatataatt taaaatagta
     1861 atgtggtgtt actgtatttg tcatataacg caatatatac agtatgtctt tatctgtatc
     1921 aattttgtgg tcacaccatc aataaactcc agtttaatta tttaaaattg ggtagtgata
     1981 accatataaa ggaacagtgg ctagttccat gttgtagctc ccaacctgct ttcaggtgat
     2041 ccatgcagtg tcgtataaaa aggtaacaat ttttaacctt ggaaagcaag tacacttaag
     2101 gtcccagatc tgaagctacc cctaacacac tattctacac acagaaaatg ctcttcacta
     2161 ggtcgagacc agcaaagttc tagaatttgt cgaaacattt atgttatata tttcctgaaa
     2221 aaaattctga gtaagttctt aagtgttatt gccagcaaca taaacaacag acggcaaaat
     2281 gaataaatga taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta
     2341 caaccctcaa acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa
     2401 gaaaattggt tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag
     2461 tgacctgatt ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa
     2521 ggcacagcac tcactagctg ccctatatat tttattatat tttacatata ttattttatt
     2581 tatttagctc tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg
     2641 tactacctca ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc
     2701 atgcttgttt tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga
     2761 tgacatgcga cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa
     2821 atcatataat tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc
     2881 aacagatagt cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg
     2941 cgttctatat ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca
     3001 aacaatgtag aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc
     3061 tttgtgaaaa catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa
     3121 tagaacatat acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta
     3181 ttgctggcag ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg
     3241 gtccagattc cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca
     3301 atcactgctt gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc
     3361 tttatatgat aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac
     3421 ataaccatag caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga
     3481 aagatctggt catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta
     3541 ggactctcga ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt
     3601 tccgtttcaa cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt
     3661 ctttaaaacc atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga
     3721 gacgttttgt gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg
     3781 aggaccagta acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg
     3841 ttaacgtcaa ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt
     3901 cagtattttt aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg
     3961 aacaaagaac ccggatgtta catacagtac agccgccatg ttacagagag ttataactta
     4021 atcattttaa aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg
     4081 ttaaaatgag gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca
     4141 ctgaactgga gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca
     4201 ttgtagatgt tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt
     4261 gtaataacag taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg
     4321 ctctgtaagc tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt
     4381 gaattaaata aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa
     4441 ataagaaaaa atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa
     4501 ataccttttt gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag
     4561 tactccaaat gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt
     4621 gcaataataa ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt
     4681 gatcatgctt caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc
     4741 ttccattaca aactattccc atcttattga attctggtat gtcttaaagg attagttcac
     4801 ttcccaaatc aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa
     4861 tgaagaaatt gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta
     4921 gtttaaaatt ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg
     4981 aataacagtc ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt
     5041 tatttttaaa tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc
     5101 cttccttact ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg
     5161 gaacattatt attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg
     5221 cacagatgtg cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat
     5281 ttcatgatgc acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg
     5341 taagacccta aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt
     5401 ctatagtcca ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc
     5461 aatagttttc tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa
     5521 atttcatttt cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt
     5581 ggctagaaca ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat
     5641 ttgtttacag ggatctgatt taggtgacac tatagaatac aagctacttg ttctttttgc
     5701 aggtaccatg gcgtacaaga ctagctcgga cagtcgggac ccagccagcg accagatgaa
     5761 gcagtggaag gagcagcggg cctcgcagag acctgatgtc ctgaccaccg gaggcgggaa
     5821 cccaatagga gataaactta atatcatgac cgcggggtcc cgagggcccc tcctcgttca
     5881 ggatgtggtt ttcactgacg agatggcaca ctttgacaga gagcggattc ctgagagagt
     5941 ggtacacgca aaaggagcag gtgcttttgg atactttgag gtcacccacg atatcaccag
     6001 atactccaag gcaaaggtgt ttgagcatat tggaaagagg acccctattg ccgttcgatt
     6061 ctccacagtc gctggagagt caggctcagc tgacacagtt cgtgaccctc gggggtttgc
     6121 agtgaaattt tacactgaag atggtaactg ggatcttgtg ggaaacaaca cccctatttt
     6181 cttcatcagg gatgccatat tgtttccatc ctttatccat agccagaaga gaaacccaca
     6241 gactcacctg aaggatcctg acatggtctg ggacttctgg agtcttcgtc ccgagtctct
     6301 ccatcaggtt tctttcttgt tcagtgaccg agggattccc gatggtcacc ggcacatgaa
     6361 tggctatgga tcacacacct tcaagttggt taatgcagat ggagaggcag tctattgcaa
     6421 gttccattac aagaccgacc agggcatcaa aaacttgcct gttggagagg caggaaggct
     6481 tgctcaggaa gatccggatt atggcctccg agatcttttc aatgccatcg ccaatggcaa
     6541 ttacccgtcc tggacgtttt acatccaggt catgactttt aaggaggcag aaactttccc
     6601 atttaatcca tttgatctga ccaaggtttg gcctcacaag gactaccctc ttataccagt
     6661 tggcaaactg gttttaaaca aaaatccagt taattacttt gctgaagttg aacagatggc
     6721 ttttgaccca agcaatatgc cccctggcat cgagcccagc cctgacaaaa tgcttcaggg
     6781 ccgccttttt gcctacccgg acactcaccg ccaccgcctg ggacccaact atctgcagat
     6841 acctgtgaac tgtccctacc gcgctcgagt ggccaactac cagcgtgatg gccccatgtg
     6901 catgcatgac aaccagggtg gtgcccccaa ctattacccc aacagcttca gcgcaccaga
     6961 gcagcagcgc tcagccctgg agcacagcgt ccagtgcgct gtagatgtga aacgcttcaa
     7021 cagtgctaat gaagacaatg tcactcaggt gcggacattc tacacaaagg tgttgaacga
     7081 ggaggagagg aaacgcctgt gtgagaacat tgccggccac ctgaaggacg ctcagctttt
     7141 cattcagaag aaagcggtca agaatttcac tgacgtccac cctgactatg gggcccgcat
     7201 ccaggctctt ctggacaagt acaacgctga gaagcctaag aacgcaattc acacctacac
     7261 gcaggcccaa gctcatggcg ggccagagaa cctgtacttc caggggctcg agccctctgc
     7321 cggagacatg agagctgcaa acctttggcc gagcccgctg atgattaaac gctctaagaa
     7381 gaacagcctg gccttgtccc tgaccgccga ccagatggtc agtgccttgt tggatgccga
     7441 gccccccata ctctattccg agtatgatcc taccagaccc ttcagtgagg cttcgatgat
     7501 gggcttactg accaacctgg cagacaggga gctggttcac atgattaact gggccaagag
     7561 ggtgccaggc tttgtggatt tgaccctcca cgatcaggtc cacctcctag aatgtgcctg
     7621 gctagagatc ctgatgattg gtctcgtctg gcgcagcatg gagcacccag tgaagctact
     7681 gtttgctcct aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga
     7741 gattttcgac atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga
     7801 ggaatttgtg tgcctcaaat ctattatttt gcttaattct ggagtttaca catttctgtc
     7861 cagcaccctg aagtctctgg aggaaaagga ccatatccat cgagtgctgg acaagatcac
     7921 agacactttg attcacctga tggctaaggc tggcctgacc ctgcagcagc agcatcagag
     7981 actcgcacag cttctcctga tcctctccca catcaggcac atgagtaaca aaggcatgga
     8041 gcatctgtac tctatgaagt gcaagaacgt ggtgcccctc tatgatctgc tgctggaggc
     8101 ggcggacgct caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggaaac
     8161 agaccaaagc cacttggcta ctgcgggctc tacttcatct cattccttgc aaaagtatta
     8221 catcacgggg gaggcagagg gtttccctgc cacagcacca ggttccggca gcgctacaaa
     8281 cttctctctg ctcaagcagg ctggagatgt ggaagaaaac cctgggcccg aagacggcat
     8341 ggtgagcaag ggcgaggagg ataacatggc tatcatcaag gagttcatgc gcttcaaggt
     8401 tcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     8461 cccctacgag ggcacccaga ccgccaagct gaaggtgaca aagggcggcc ccctgccctt
     8521 cgcctgggac atcctgagcc ctcagttcat gtacggctcc aaagcctacg tgaagcaccc
     8581 cgccgacatc cccgactact tgaagctgtc tttcccagag ggcttcaagt gggagcgcgt
     8641 gatgaacttc gaggacggcg gcgtggtgac agtgacccag gactcttctc tgcaggacgg
     8701 cgagtttatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     8761 gcagaagaaa actatgggat gggaggcttc ttccgagcgg atgtacccag aggacggagc
     8821 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgatgccga
     8881 ggtcaagacc acctacaagg ccaagaagcc cgtgcaactg cccggagcct acaacgtcaa
     8941 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     9001 cgccgagggc cgccacagca ccggcggcat ggacgagctg tacaagtaag gcgcgccgat
     9061 atctacgtag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     9121 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccatatta
     9181 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     9241 gaggtgtggg aggttttttc ctgcaggact ctagtcgcgg ccgctcttcc gctaatactc
     9301 aagtacaatt ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac
     9361 ttttactttt aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt
     9421 tttgagtact ttttacacct ctgccctgca ttaatgaatc ggccaacgcg cggggagagg
     9481 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9541 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9601 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9661 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9721 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9841 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9901 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9961 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10021 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10081 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10141 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10201 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10261 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10321 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10381 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10441 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10501 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10561 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10621 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10681 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10741 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10801 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10861 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10921 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10981 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11041 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11101 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11161 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11221 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11281 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11341 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11401 tccgcgcaca tttccccgaa aagtgccacc t
//
