LOCUS       sequence-363963-       10566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible GFP nuclear relocation in zebrafish permanent 
            transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Zhang W, Hamouri F, Feng Z, Aujard I, Ducos B, Ye S, Weiss S, 
            Volovitch M, Vriz S, Jullien L, Bensimon D
  TITLE     Control of Protein Activity and Gene Expression by Cyclofen-OH 
            Uncaging.
  JOURNAL   Chembiochem. 2018 Jun 18;19(12):1232-1238. doi: 
            10.1002/cbic.201700630. Epub 2018 Feb 27.
  PUBMED    29341391
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201700630"; journalName: "Chembiochem"; date: 
            "2018-06-18- 18"; volume: "19"; issue: "12"; pages: "1232-1238"
COMMENT     Sequence Label: pT22UbGfpnE4PCh(#301)
FEATURES             Location/Qualifiers
     source          1..10566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    397..518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(538..1245,1246..1248,1249..1251))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             complement(2012..2023)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      5662..5671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5668..5670,5671..5673,5674..6381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(5668..5670,5671..5673,5674..6381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             6388..6408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6421..6441
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7408..7464
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7474..8184
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8212..8333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8429..8566,8567..8578)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8646..8663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8817..9405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8897..8916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9576..10367,10368..10436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10199..10218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10437..10541)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcaggaccag acatgataag atacattgat gagtttggac
      421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      481 ctttatttgt aaccattata agctgcaata aacaagttgc gtgcgcattt atttaaggta
      541 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      601 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      661 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      721 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      781 cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct tgtgccccag
      841 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      901 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      961 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1021 gtcggggtag cggctgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
     1081 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1141 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1201 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgtcggctgg
     1261 cgaccggtgg atcatccccc gcaagtctga ctgagtggaa gtttcagttc aatggaaggg
     1321 agaaaagagc cagcctttat atatgtcatg tattgctgct gtctgcatgc gatgaaccta
     1381 gagcttactt tttctgtcaa gtaaggcaaa agcagagaac ctttgtcttg cattgtgttt
     1441 gatgttgtgc tgtgacagtc atttcttagt tcatgctggt acttttgttc catagcttta
     1501 cccaaagagt tatccagcat tcccagttat tccaaaaact caatgtcaca aattttccag
     1561 tccattgctt agcagcgttg accccggaga ccctgatgtg gtaatttaca gtgcaattca
     1621 gttgctggtc caaacacttt gtaacagttt aaagaattgt ggttactagt aaatccttaa
     1681 tttggcatca tctaggtgtc attctttgcc acttttgaat cacacaaatg aaccaaacaa
     1741 tggttagcta gcatcaaaca tttaccaaat aatctcatca gtgtcacccc aatgtttaag
     1801 aagccacatt ttatgtcccc agatatatta atattatttt atatataatt taaaatagta
     1861 atgtggtgtt actgtatttg tcatataacg caatatatac agtatgtctt tatctgtatc
     1921 aattttgtgg tcacaccatc aataaactcc agtttaatta tttaaaattg ggtagtgata
     1981 accatataaa ggaacagtgg ctagttccat gttgtagctc ccaacctgct ttcaggtgat
     2041 ccatgcagtg tcgtataaaa aggtaacaat ttttaacctt ggaaagcaag tacacttaag
     2101 gtcccagatc tgaagctacc cctaacacac tattctacac acagaaaatg ctcttcacta
     2161 ggtcgagacc agcaaagttc tagaatttgt cgaaacattt atgttatata tttcctgaaa
     2221 aaaattctga gtaagttctt aagtgttatt gccagcaaca taaacaacag acggcaaaat
     2281 gaataaatga taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta
     2341 caaccctcaa acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa
     2401 gaaaattggt tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag
     2461 tgacctgatt ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa
     2521 ggcacagcac tcactagctg ccctatatat tttattatat tttacatata ttattttatt
     2581 tatttagctc tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg
     2641 tactacctca ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc
     2701 atgcttgttt tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga
     2761 tgacatgcga cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa
     2821 atcatataat tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc
     2881 aacagatagt cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg
     2941 cgttctatat ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca
     3001 aacaatgtag aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc
     3061 tttgtgaaaa catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa
     3121 tagaacatat acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta
     3181 ttgctggcag ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg
     3241 gtccagattc cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca
     3301 atcactgctt gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc
     3361 tttatatgat aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac
     3421 ataaccatag caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga
     3481 aagatctggt catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta
     3541 ggactctcga ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt
     3601 tccgtttcaa cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt
     3661 ctttaaaacc atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga
     3721 gacgttttgt gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg
     3781 aggaccagta acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg
     3841 ttaacgtcaa ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt
     3901 cagtattttt aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg
     3961 aacaaagaac ccggatgtta catacagtac agccgccatg ttacagagag ttataactta
     4021 atcattttaa aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg
     4081 ttaaaatgag gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca
     4141 ctgaactgga gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca
     4201 ttgtagatgt tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt
     4261 gtaataacag taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg
     4321 ctctgtaagc tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt
     4381 gaattaaata aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa
     4441 ataagaaaaa atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa
     4501 ataccttttt gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag
     4561 tactccaaat gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt
     4621 gcaataataa ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt
     4681 gatcatgctt caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc
     4741 ttccattaca aactattccc atcttattga attctggtat gtcttaaagg attagttcac
     4801 ttcccaaatc aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa
     4861 tgaagaaatt gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta
     4921 gtttaaaatt ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg
     4981 aataacagtc ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt
     5041 tatttttaaa tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc
     5101 cttccttact ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg
     5161 gaacattatt attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg
     5221 cacagatgtg cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat
     5281 ttcatgatgc acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg
     5341 taagacccta aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt
     5401 ctatagtcca ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc
     5461 aatagttttc tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa
     5521 atttcatttt cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt
     5581 ggctagaaca ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat
     5641 ttgtttacag ggatcccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     5701 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     5761 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5821 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5881 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5941 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     6001 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     6061 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     6121 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     6181 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6241 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     6301 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     6361 ctcggcatgg acgagctgta caggatcccc aagaagaagc gcaaggtggc gcccgggcca
     6421 gagaacctgt acttccaggg gctcgagccc tctgccggag acatgagagc tgcaaacctt
     6481 tggccgagcc cgctgatgat taaacgctct aagaagaaca gcctggcctt gtccctgacc
     6541 gccgaccaga tggtcagtgc cttgttggat gccgagcccc ccatactcta ttccgagtat
     6601 gatcctacca gacccttcag tgaggcttcg atgatgggct tactgaccaa cctggcagac
     6661 agggagctgg ttcacatgat taactgggcc aagagggtgc caggctttgt ggatttgacc
     6721 ctccacgatc aggtccacct cctagaatgt gcctggctag agatcctgat gattggtctc
     6781 gtctggcgca gcatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac
     6841 aggaaccagg gaaaatgtgt agagggcatg gtggagattt tcgacatgct gctggctaca
     6901 tcatctcggt tccgcatgat gaatctgcag ggagaggaat ttgtgtgcct caaatctatt
     6961 attttgctta attctggagt ttacacattt ctgtccagca ccctgaagtc tctggaggaa
     7021 aaggaccata tccatcgagt gctggacaag atcacagaca ctttgattca cctgatggct
     7081 aaggctggcc tgaccctgca gcagcagcat cagagactcg cacagcttct cctgatcctc
     7141 tcccacatca ggcacatgag taacaaaggc atggagcatc tgtactctat gaagtgcaag
     7201 aacgtggtgc ccctctatga tctgctgctg gaggcggcgg acgctcaccg cctacatgcg
     7261 cccactagcc gtggaggggc atccgtggag gaaacagacc aaagccactt ggctactgcg
     7321 ggctctactt catctcattc cttgcaaaag tattacatca cgggggaggc agagggtttc
     7381 cctgccacag caccaggttc cggcagcgct acaaacttct ctctgctcaa gcaggctgga
     7441 gatgtggaag aaaaccctgg gcccgaagac ggcatggtga gcaagggcga ggaggataac
     7501 atggctatca tcaaggagtt catgcgcttc aaggttcaca tggagggctc cgtgaacggc
     7561 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     7621 aagctgaagg tgacaaaggg cggccccctg cccttcgcct gggacatcct gagccctcag
     7681 ttcatgtacg gctccaaagc ctacgtgaag caccccgccg acatccccga ctacttgaag
     7741 ctgtctttcc cagagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     7801 gtgacagtga cccaggactc ttctctgcag gacggcgagt ttatctacaa ggtgaagctg
     7861 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaactat gggatgggag
     7921 gcttcttccg agcggatgta cccagaggac ggagccctga agggcgagat caagcagagg
     7981 ctgaagctga aggacggcgg ccactacgat gccgaggtca agaccaccta caaggccaag
     8041 aagcccgtgc aactgcccgg agcctacaac gtcaacatca agttggacat cacctcccac
     8101 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca cagcaccggc
     8161 ggcatggacg agctgtacaa gtaaggcgcg ccgatatcta cgtagacatg ataagataca
     8221 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8281 tttgtgatgc tattgcttta tttgtaacca tattaagctg caataaacaa gttaacaaca
     8341 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcctgca
     8401 ggactctagt cgcggccgct cttccgctaa tactcaagta caattttaat ggagtacttt
     8461 tttactttta ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt
     8521 tccctaagta cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgcc
     8581 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8641 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8701 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8761 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8821 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8881 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8941 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9001 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9061 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9121 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9181 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9241 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9301 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9361 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9421 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9481 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9541 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9601 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9661 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9721 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9781 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9841 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9901 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9961 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10021 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10081 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10141 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10201 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10261 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10321 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10381 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10441 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10501 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10561 ccacct
//
