LOCUS       sequence-363974-       13256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of human TBX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13256)
  AUTHORS   Gastfriend BD, Snyder ME, Holt HE, Daneman R, Palecek SP, Shusta EV
  TITLE     Notch3 directs differentiation of brain mural cells from human 
            pluripotent stem cell-derived neural crest.
  JOURNAL   Sci Adv. 2024 Feb 2;10(5):eadi1737. doi: 10.1126/sciadv.adi1737. 
            Epub 2024 Feb 2.
  PUBMED    38306433
REFERENCE   2  (bases 1 to 13256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-2"; volume: "10(5):eadi1737. doi"; pages: " 
            10.1126/sciadv.adi1737. Epub 2024 Feb 2"
COMMENT     Sequence Label: pWPI-TBX2
FEATURES             Location/Qualifiers
     source          1..13256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2144..3322
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2375..3313
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3270..3290
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5523..5640
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5689..6256
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5707..6258
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5851..5868)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6078..6097
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6261..6263,6264..6266,6267..6977)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6261..6263,6264..6266,6267..6977)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6306..6327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6567..6586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6914..6935
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7056..7072)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7057..7073)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7086..7674
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7139..7159)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7557..7568)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7818..7851)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7871..8051
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8076..8094)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8077..8094)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8384..8403)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8617..8636)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8736..8758
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8796..8814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8882..8986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8987..9055,9056..9847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9205..9224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10018..10606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10507..10526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10760..10777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10847..10867)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10852..11181
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11032..11167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12315..12380
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12510..12530
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12955..13089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12992..13011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13046..13065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgt cgacgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
     2101 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     2161 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2221 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2281 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2341 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2401 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2461 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2521 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2581 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2641 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2701 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2761 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2821 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2881 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2941 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3001 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3061 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3121 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3181 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3241 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3301 tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaag ccaccatgag
     3361 agagccggcg ctggcggcca gcgccatggc ttaccacccg ttccacgcgc cacggcccgc
     3421 cgacttcccc atgtccgcct ttctggcggc ggcgcagccc tccttcttcc cggcactcgc
     3481 gctgccgccc ggcgcgctgg ccaagccgct gcccgacccg ggcctggcgg gggcggcggc
     3541 cgcggcggcg gcggcggcag cagcggccga ggcggggctg cacgtctcgg cactgggccc
     3601 gcacccgccc gccgcgcatc tgcgctccct caagagcctg gagcccgagg acgaggtgga
     3661 ggacgacccc aaggtgacgc tggaggccaa ggagctgtgg gaccagttcc acaagctagg
     3721 cacggagatg gtcatcacca agtccgggag gcggatgttc ccccccttca aggtgcgagt
     3781 cagcggcctg gacaagaagg ccaagtatat cctgctgatg gacattgtag ccgctgacga
     3841 ttgccgctat aagttccaca actcgcgctg gatggtggcg ggcaaggccg accctgagat
     3901 gcccaaacgc atgtacatcc acccagacag cccagccacg ggggagcagt ggatggctaa
     3961 gcctgtggcc ttccacaagc tgaagctgac caacaacatc tctgacaagc acggcttcac
     4021 catcctaaac tccatgcaca agtaccagcc gcgcttccac atagtgcgag ccaacgacat
     4081 cctgaagctg ccttacagca ccttccgcac ctacgtgttc ccggagaccg acttcatcgc
     4141 cgtcactgcc taccagaatg acaagatcac acagctgaag atcgacaaca acccgtttgc
     4201 caagggcttc cgggacaccg ggaacggccg gcgggagaaa aggaagcagc tgacgctgcc
     4261 gtctctacgc ttgtacgagg agcactgcaa acccgagcgc gatggcgcgg agtcagacgc
     4321 ctcgtcgtgc gaccctcccc ccgcgcggga accacccacc tccccgggcg cagcgcccag
     4381 tccgctgcgc ctgcaccggg cccgagctga ggagaagtcg tgcgccgcgg acagcgaccc
     4441 ggagcctgag cggttgagcg aggagcgtgc gggggcgccg ctaggccgca gcccggctcc
     4501 agacagcgcc agccccactc gcttgaccga acccgagcgc gcccgggagc ggcgtagtcc
     4561 cgagaggggc aaggagccgg ccgagagcgg cggggacggc ccgttcggcc tgaggagcct
     4621 ggagaaggag cgcgccgaag ctcggaggaa ggacgagggg cgcaaggagg cggccgaggg
     4681 caaggagcag ggcctggcgc cgctggtggt gcagacagac agtgcgtccc ccctgggcgc
     4741 cggacacctg cccggcctgg ccttttccag ccacttgcac gggcagcagt tctttgggcc
     4801 gctgggagcc ggccagccgc tcttcctgca ccctggacag ttcaccatgg gccctggcgc
     4861 cttctccgcc atgggcatgg gtcacctact ggcctcggtg gcaggcggcg gcaacggcgg
     4921 aggtggcggg cctgggaccg ccgcggggct ggacgcaggc gggctgggtc ccgcggccag
     4981 cgcagcaagc accgccgcgc ccttcccgtt ccacctctcc cagcacatgc tggcatctca
     5041 gggaattcca atgcccactt tcggaggcct cttcccctac ccctacacct acatggcagc
     5101 agcagccgca gccgcctcgg ctttgcccgc cactagtgct gcagctgccg ccgccgcagc
     5161 cgccggctcc ctctcccgga gccccttcct gggcagtgcc cggccccgac tgcgtttcag
     5221 cccctatcag atcccggtca ccatcccgcc tagcactagc ctcctcacca ccgggctggc
     5281 ctctgagggc tccaaggccg ctggtggaaa cagccgggag cctagccccc tgcccgagct
     5341 ggctctccgc aaagtagggg ccccatcccg cggtgccctg tcgcccagtg gctcggccaa
     5401 ggaggcggcc aatgaactgc agagcatcca gagactggtg agtgggctgg agagccagcg
     5461 agccctctcc ccaggccggg agtcgcccaa gtgattaatt aatctcgacg gtatcggtta
     5521 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     5581 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     5641 atcgatcacg agactagcct cgaggtttaa actacgggct gcaggaattc cgnccccccc
     5701 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5761 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5821 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5881 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5941 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6001 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6061 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6121 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6181 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6241 aaaaacacga tgataatacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     6301 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     6361 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     6421 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     6481 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     6541 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     6601 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6661 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6721 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     6781 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     6841 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     6901 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     6961 tggacgagct gtacaagtcc ggactcagat ctcgactagc tagtagctag ctagctagtc
     7021 gagctcaagc ttcgaattcg atatcaagct tatcgcgata ccgtcgacct cgagggaatt
     7081 ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7141 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7201 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7261 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7321 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7381 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7441 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7501 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7561 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7621 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg
     7681 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7741 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatggg
     7801 atcaattcac catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt
     7861 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7921 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7981 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8041 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     8101 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     8161 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     8221 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     8281 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     8341 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     8401 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     8461 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     8521 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     8581 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     8641 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8701 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8761 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     8821 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8881 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8941 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9001 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9061 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9121 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9181 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9241 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9301 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9361 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9421 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9481 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9541 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9601 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9661 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9721 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9781 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9841 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9901 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9961 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10021 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10081 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10141 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10381 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10621 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10681 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10741 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10801 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
    10861 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10921 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10981 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    11041 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    11101 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    11161 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
    11221 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
    11281 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    11341 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    11401 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    11461 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    11521 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    11581 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    11641 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    11701 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    11761 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    11821 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    11881 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    11941 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    12001 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    12061 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    12121 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    12181 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    12241 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    12301 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    12361 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    12421 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    12481 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    12541 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
    12601 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
    12661 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
    12721 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
    12781 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
    12841 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    12901 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    12961 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    13021 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    13081 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    13141 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    13201 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
