LOCUS       sequence-363975-       10921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible chicken EN2 (mut) activation in zebrafish 
            permanent transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Rampon C, Gauron C, Lin T, Meda F, Dupont E, Cosson A, Ipendey E, 
            Frerot A, Aujard I, Le Saux T, Bensimon D, Jullien L, Volovitch M, 
            Vriz S, Joliot A
  TITLE     Control of brain patterning by Engrailed paracrine transfer: a new 
            function of the Pbx interaction domain.
  JOURNAL   Development. 2015 May 15;142(10):1840-9. doi: 10.1242/dev.114181. 
            Epub 2015 Apr 29.
  PUBMED    25926358
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.114181"; journalName: "Development"; date: "2015-05-15-
            15"; volume: "142"; issue: "10"; pages: "1840-9"
COMMENT     Sequence Label: pT22Ubm5En2SRE4PCh(#302)
FEATURES             Location/Qualifiers
     source          1..10921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(152..172)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(152..170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(181..192,193..380)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    399..520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(540..1247,1248..1250,1251..1253))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(579..600)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     928..947
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1187..1208
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2014..2025)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5677..5706
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5716..5745
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5755..5784
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5794..5823
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5833..5862
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6778..6798
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7765..7821
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7831..8541
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8569..8690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8786..8923,8924..8935)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9003..9020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9174..9762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9254..9273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9933..10724,10725..10793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10556..10575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10794..10898)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc ccgatactcc agcttttgtt ccctttagtg agggttaatt gcgcgttaca
      181 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
      241 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
      301 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
      361 ttattttttg gagatcactt cctcaggacc agacatgata agatacattg atgagtttgg
      421 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      481 tgctttattt gtaaccatta taagctgcaa taaacaagtt gcgtgcgcat ttatttaagg
      541 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      601 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      661 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      721 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc
      781 tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag cttgtgcccc
      841 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      901 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      961 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     1021 tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac gagggtgggc
     1081 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     1141 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     1201 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgtcggct
     1261 ggcgaccggt ggatcatccc ccgcaagtct gactgagtgg aagtttcagt tcaatggaag
     1321 ggagaaaaga gccagccttt atatatgtca tgtattgctg ctgtctgcat gcgatgaacc
     1381 tagagcttac tttttctgtc aagtaaggca aaagcagaga acctttgtct tgcattgtgt
     1441 ttgatgttgt gctgtgacag tcatttctta gttcatgctg gtacttttgt tccatagctt
     1501 tacccaaaga gttatccagc attcccagtt attccaaaaa ctcaatgtca caaattttcc
     1561 agtccattgc ttagcagcgt tgaccccgga gaccctgatg tggtaattta cagtgcaatt
     1621 cagttgctgg tccaaacact ttgtaacagt ttaaagaatt gtggttacta gtaaatcctt
     1681 aatttggcat catctaggtg tcattctttg ccacttttga atcacacaaa tgaaccaaac
     1741 aatggttagc tagcatcaaa catttaccaa ataatctcat cagtgtcacc ccaatgttta
     1801 agaagccaca ttttatgtcc ccagatatat taatattatt ttatatataa tttaaaatag
     1861 taatgtggtg ttactgtatt tgtcatataa cgcaatatat acagtatgtc tttatctgta
     1921 tcaattttgt ggtcacacca tcaataaact ccagtttaat tatttaaaat tgggtagtga
     1981 taaccatata aaggaacagt ggctagttcc atgttgtagc tcccaacctg ctttcaggtg
     2041 atccatgcag tgtcgtataa aaaggtaaca atttttaacc ttggaaagca agtacactta
     2101 aggtcccaga tctgaagcta cccctaacac actattctac acacagaaaa tgctcttcac
     2161 taggtcgaga ccagcaaagt tctagaattt gtcgaaacat ttatgttata tatttcctga
     2221 aaaaaattct gagtaagttc ttaagtgtta ttgccagcaa cataaacaac agacggcaaa
     2281 atgaataaat gataacaaag cagtaggctt aaataaacct aatttttata ggctgttctc
     2341 tacaaccctc aaacagtgat tagttttgta cttataaact tgccctttca ttcatatttc
     2401 aagaaaattg gttcagaaga tctggatatt ctagcagttg ttcaagctca tggagggatc
     2461 agtgacctga ttccacaatg actaggccta atccagaaat tagatgactg tcaacataaa
     2521 aaggcacagc actcactagc tgccctatat attttattat attttacata tattatttta
     2581 tttatttagc tctgagtgct gtactttctg gttaaagaaa actgcttaca acagctaacc
     2641 tgtactacct caggctcagg gaatttggaa caggtttgtc tggtttgttt ctttaaccat
     2701 gcatgcttgt tttcaactat ggcaacacag tcacatggga cattacagaa atgatttgtc
     2761 gatgacatgc gacttttctt taataaagcg caaagatccc aaaaagcaaa cttttaacaa
     2821 aaatcatata attatatttt caatccagct ttgtagcaac tttgtgctgc tgttcactca
     2881 gcaacagata gtcagtataa ggtcagtgtg tctcaaagca gtgccatctg tttcacacat
     2941 tgcgttctat atataagtgt gctggttgac acgacactgt ataaggccta ggctaaaaca
     3001 caaacaatgt agaatgacac tgtgtttttt ttgtaaacaa atgttgtttt tggttaaaca
     3061 tctttgtgaa aacatcctcc tgtcatgtat ttgctatatt caaatgttaa acccgtgcag
     3121 aatagaacat atacaaaaaa aaacaacaca acacattttt aaacattatt aaatatcaag
     3181 tattgctggc agttctgttt ctgttttaca gtaccctttg ccacagttct ccgcttttcc
     3241 tggtccagat tccacaagtc tgattcacca atagcaaagc gaataaacaa ccaaagcagc
     3301 caatcactgc ttgtagactg tcctgcgaga ccggcccatt ccagcacatt ctggaaactt
     3361 cctttatatg ataattataa atacatttaa attattgata caaaacatgt aattcctaga
     3421 acataaccat agcaatcatt agttttcagg gtaattatgt atttttagga tttgactgcg
     3481 gaaagatctg gtcatgtgac gtctcatgaa cgtcacggcc ctgggtttct ataaatacag
     3541 taggactctc gaccatcggc agatttttcg aagaagaaga tcagtttcag gagccgtact
     3601 gttccgtttc aacgcaaata ttaacggtaa gagcgaattt cctagtttgt tttcatgcca
     3661 ttctttaaaa ccatagcgta ttactttaat tatagtaaac tttcgctttc tttattacaa
     3721 gagacgtttt gtgttgattc tccgcggaca ttttcggtca gacaatcaga aaatgaccgc
     3781 ggaggaccag taacttgcat tacacgtaag ttaaatcttc gtgtattaaa atggttaggt
     3841 tgttaacgtc aaataggtta ccgtgtttgc gtgtgatcag gttggttttg ttagattttt
     3901 gtcagtattt ttaatttatt tgttttagtt tatttatttt ttttgctgaa tcatagtttg
     3961 tgaacaaaga acccggatgt tacatacagt acagccgcca tgttacagag agttataact
     4021 taatcatttt aaaaataatt ttgccttact tttagtttgt catgttgaga aatgaggaaa
     4081 tgttaaaatg aggaaatatc caattaattt aatatatcaa aataatccat gattacaatg
     4141 cactgaactg gagaaaatta agatgttttc tagtgtcatg aaacaaatgt aagagatgta
     4201 cattgtagat gttttatgtc aagaattggc tagttgatgc agcatactgg cgatactcag
     4261 ttgtaataac agtaacgtta catgttaata gactactgag tatgctgttc tgtctatgta
     4321 tgctctgtaa gctacgagaa ggactttttt aaacagtaaa gggtgcaata tttttacaaa
     4381 ttgaattaaa taaaggctgt ctattaagta atatgcttga tatttttctt acttgatcgg
     4441 aaataagaaa aaatataaac gttgttgctc taaaaatcct agttcagttt agccaaccac
     4501 aaataccttt ttgttcctcc aacagttttt ttttcttctc tataatattt ggcagtctat
     4561 agtactccaa atgtttcccc acagtctaac taattggtac agccaaaatc atgacactta
     4621 ttgcaataat aattttggtt cattggcatt gttgatagcc tgtgccacta atatggtcga
     4681 ttgatcatgc ttcaggaaga aaactatatt gtttgatgta agattattaa atcttcacct
     4741 gcttccatta caaactattc ccatcttatt gaattctggt atgtcttaaa ggattagttc
     4801 acttcccaaa tcaaaattta cttagccttt tttcatccat gatccctttt tttcatcatt
     4861 aatgaagaaa ttgtttttga aaaagtttca agattttttt ctctatattg tggagcttgt
     4921 tagtttaaaa ttccaaaatg caatatgtgg cttcaaatgg ttctaaatga tcccagtcaa
     4981 ggaataacag tcttatctaa tgaaaccatt agacctttaa aaataaaaaa aataaaagta
     5041 tttattttta aatgactgag tgattaagtt gaatttcagc gtttccttac tgtgtagaag
     5101 tccttcctta ctggccccac cctttggttc tctgccaatc tgctacctaa tgtaatgttg
     5161 tggaacatta ttattcttta tttcttaatt ttttattttt tattttaaaa caatgtaaac
     5221 tgcacagatg tgcagtttgt ttaaaatggc caatgctttg gaaatgcatg acataattag
     5281 atttcatgat gcacaaagcc aaatctcaga gcttgtgcaa aatgagctat catttcacta
     5341 ggtaagaccc taaattttca tataggatca tttggacaat tttgctgcag gtaaaatgca
     5401 ttctatagtc cactgtcagc cattgttttg gatagtattt atttttctct acaagtatag
     5461 tcaatagttt tctattattt taaaggtttg taacatttaa gggtgaccaa atgcaaagta
     5521 aaatttcatt ttcgggtgaa ctatctcgtt taacatggga gaagtgcaaa acatacatta
     5581 ttggctagaa cattgtagta ttttttaaat ggaaatgtgt gattgctaat cttactttga
     5641 atttgtttac agggatccca tcgatttaaa gctatggagc aaaagctcat ttctgaagag
     5701 gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa
     5761 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac
     5821 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagagcttg
     5881 ggcgacctca ccatggagga gggcggcagg tcgccccggg aggaggcggc cgaaccgcag
     5941 gagtccggcg gggacgcgga acccggcgga gggcgtcggg ctctgctgct gccccccggc
     6001 gaccccccgc acccccaccc gcaccctcac cgcatcacca acttcttcat cgacaacatc
     6061 ctgaggcccg agttcggtcg gaggaaggaa gcgggaggaa ccgccggaga accccgcagg
     6121 cccggagcgg agagcaggag gagccccgcg gccgccgcgc ccgctcccgg agctccggtg
     6181 cccggcggag gaggcggcgg cgggggtgga tcgccgggcc gaggggaggg cggccccgcc
     6241 gctctggccc tgcacggagc ggccaagaag gggggagacc ccgcggcgct ggaggcggcc
     6301 ctgaaagcgc gggggctgag cggcgcggag ctgtcggtga gctcggactc ggatagctcc
     6361 caggccggct ccaacgccgg gaaccaaccc atgctttggc ccgcttgggt gtactgcacg
     6421 cggtactcgg accgaccgtc ttcaggtccc cgctcccgca aaccaaagaa gaagaacccc
     6481 aacaaggaag acaagcggcc ccgcaccgcc ttcacggccg agcagctgca gagactcaag
     6541 gccgagttcc agacgaaccg ctacctgacg gagcagcgcc ggcagagcct ggcccaggag
     6601 ctcggcctca acgagtccca gatcaagatc tctagacaga acaagagagc caagatcaag
     6661 aaggcgacgg gcagcaagaa ctcgctggcg gtgcacctca tggcccaggg gctctacaac
     6721 cactccacca cggcgaaaga cggcaagtcg gacagcgaag ctttggcgcc cgggccagag
     6781 aacctgtact tccaggggct cgagccctct gccggagaca tgagagctgc aaacctttgg
     6841 ccgagcccgc tgatgattaa acgctctaag aagaacagcc tggccttgtc cctgaccgcc
     6901 gaccagatgg tcagtgcctt gttggatgcc gagcccccca tactctattc cgagtatgat
     6961 cctaccagac ccttcagtga ggcttcgatg atgggcttac tgaccaacct ggcagacagg
     7021 gagctggttc acatgattaa ctgggccaag agggtgccag gctttgtgga tttgaccctc
     7081 cacgatcagg tccacctcct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc
     7141 tggcgcagca tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg
     7201 aaccagggaa aatgtgtaga gggcatggtg gagattttcg acatgctgct ggctacatca
     7261 tctcggttcc gcatgatgaa tctgcaggga gaggaatttg tgtgcctcaa atctattatt
     7321 ttgcttaatt ctggagttta cacatttctg tccagcaccc tgaagtctct ggaggaaaag
     7381 gaccatatcc atcgagtgct ggacaagatc acagacactt tgattcacct gatggctaag
     7441 gctggcctga ccctgcagca gcagcatcag agactcgcac agcttctcct gatcctctcc
     7501 cacatcaggc acatgagtaa caaaggcatg gagcatctgt actctatgaa gtgcaagaac
     7561 gtggtgcccc tctatgatct gctgctggag gcggcggacg ctcaccgcct acatgcgccc
     7621 actagccgtg gaggggcatc cgtggaggaa acagaccaaa gccacttggc tactgcgggc
     7681 tctacttcat ctcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct
     7741 gccacagcac caggttccgg cagcgctaca aacttctctc tgctcaagca ggctggagat
     7801 gtggaagaaa accctgggcc cgaagacggc atggtgagca agggcgagga ggataacatg
     7861 gctatcatca aggagttcat gcgcttcaag gttcacatgg agggctccgt gaacggccac
     7921 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     7981 ctgaaggtga caaagggcgg ccccctgccc ttcgcctggg acatcctgag ccctcagttc
     8041 atgtacggct ccaaagccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     8101 tctttcccag agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     8161 acagtgaccc aggactcttc tctgcaggac ggcgagttta tctacaaggt gaagctgcgc
     8221 ggcaccaact tcccctccga cggccccgta atgcagaaga aaactatggg atgggaggct
     8281 tcttccgagc ggatgtaccc agaggacgga gccctgaagg gcgagatcaa gcagaggctg
     8341 aagctgaagg acggcggcca ctacgatgcc gaggtcaaga ccacctacaa ggccaagaag
     8401 cccgtgcaac tgcccggagc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     8461 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccacag caccggcggc
     8521 atggacgagc tgtacaagta aggcgcgccg atatctacgt agacatgata agatacattg
     8581 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     8641 gtgatgctat tgctttattt gtaaccatat taagctgcaa taaacaagtt aacaacaaca
     8701 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcctgcagga
     8761 ctctagtcgc ggccgctctt ccgctaatac tcaagtacaa ttttaatgga gtactttttt
     8821 acttttactc aagtaagatt ctagccagat acttttactt ttaattgagt aaaattttcc
     8881 ctaagtactt gtactttcac ttgagtaaaa tttttgagta ctttttacac ctctgccctg
     8941 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9001 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9061 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10921 c
//
