LOCUS       sequence-363979-       10921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible chicken EN2 (mut) activation in zebrafish 
            permanent transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Amblard I, Thauvin M, Rampon C, Queguiner I, Pak VV, Belousov V, 
            Prochiantz A, Volovitch M, Joliot A, Vriz S
  TITLE     H2O2 and Engrailed 2 paracrine activity synergize to shape the 
            zebrafish optic tectum.
  JOURNAL   Commun Biol. 2020 Sep 29;3(1):536. doi: 10.1038/s42003-020-01268-7.
  PUBMED    32994473
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-09-29"; volume: "3(1):536. doi"; pages: " 
            10.1038/s42003-020-01268-7"
COMMENT     Sequence Label: pT22gcfUbm5En25EE4PCh(#552)
FEATURES             Location/Qualifiers
     source          1..10921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    397..518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(538..1245,1246..1248,1249..1251))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(577..598)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     926..945
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1185..1206
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2012..2023)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5675..5704
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5714..5743
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5753..5782
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5792..5821
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5831..5860
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6776..6796
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7763..7819
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7829..8539
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8567..8688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8784..8921,8922..8933)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9001..9018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9172..9760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9252..9271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9931..10722,10723..10791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10554..10573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10792..10896)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcaggaccag acatgataag atacattgat gagtttggac
      421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      481 ctttatttgt aaccattata agctgcaata aacaagttgc gtgcgcattt atttaaggta
      541 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      601 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      661 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      721 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      781 cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct tgtgccccag
      841 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      901 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      961 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1021 gtcggggtag cggctgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
     1081 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1141 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1201 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgtcggctgg
     1261 cgaccggtgg atcatccccc gcaagtctga ctgagtggaa gtttcagttc aatggaaggg
     1321 agaaaagagc cagcctttat atatgtcatg tattgctgct gtctgcatgc gatgaaccta
     1381 gagcttactt tttctgtcaa gtaaggcaaa agcagagaac ctttgtcttg cattgtgttt
     1441 gatgttgtgc tgtgacagtc atttcttagt tcatgctggt acttttgttc catagcttta
     1501 cccaaagagt tatccagcat tcccagttat tccaaaaact caatgtcaca aattttccag
     1561 tccattgctt agcagcgttg accccggaga ccctgatgtg gtaatttaca gtgcaattca
     1621 gttgctggtc caaacacttt gtaacagttt aaagaattgt ggttactagt aaatccttaa
     1681 tttggcatca tctaggtgtc attctttgcc acttttgaat cacacaaatg aaccaaacaa
     1741 tggttagcta gcatcaaaca tttaccaaat aatctcatca gtgtcacccc aatgtttaag
     1801 aagccacatt ttatgtcccc agatatatta atattatttt atatataatt taaaatagta
     1861 atgtggtgtt actgtatttg tcatataacg caatatatac agtatgtctt tatctgtatc
     1921 aattttgtgg tcacaccatc aataaactcc agtttaatta tttaaaattg ggtagtgata
     1981 accatataaa ggaacagtgg ctagttccat gttgtagctc ccaacctgct ttcaggtgat
     2041 ccatgcagtg tcgtataaaa aggtaacaat ttttaacctt ggaaagcaag tacacttaag
     2101 gtcccagatc tgaagctacc cctaacacac tattctacac acagaaaatg ctcttcacta
     2161 ggtcgagacc agcaaagttc tagaatttgt cgaaacattt atgttatata tttcctgaaa
     2221 aaaattctga gtaagttctt aagtgttatt gccagcaaca taaacaacag acggcaaaat
     2281 gaataaatga taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta
     2341 caaccctcaa acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa
     2401 gaaaattggt tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag
     2461 tgacctgatt ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa
     2521 ggcacagcac tcactagctg ccctatatat tttattatat tttacatata ttattttatt
     2581 tatttagctc tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg
     2641 tactacctca ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc
     2701 atgcttgttt tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga
     2761 tgacatgcga cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa
     2821 atcatataat tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc
     2881 aacagatagt cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg
     2941 cgttctatat ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca
     3001 aacaatgtag aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc
     3061 tttgtgaaaa catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa
     3121 tagaacatat acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta
     3181 ttgctggcag ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg
     3241 gtccagattc cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca
     3301 atcactgctt gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc
     3361 tttatatgat aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac
     3421 ataaccatag caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga
     3481 aagatctggt catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta
     3541 ggactctcga ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt
     3601 tccgtttcaa cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt
     3661 ctttaaaacc atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga
     3721 gacgttttgt gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg
     3781 aggaccagta acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg
     3841 ttaacgtcaa ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt
     3901 cagtattttt aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg
     3961 aacaaagaac ccggatgtta catacagtac agccgccatg ttacagagag ttataactta
     4021 atcattttaa aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg
     4081 ttaaaatgag gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca
     4141 ctgaactgga gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca
     4201 ttgtagatgt tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt
     4261 gtaataacag taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg
     4321 ctctgtaagc tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt
     4381 gaattaaata aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa
     4441 ataagaaaaa atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa
     4501 ataccttttt gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag
     4561 tactccaaat gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt
     4621 gcaataataa ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt
     4681 gatcatgctt caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc
     4741 ttccattaca aactattccc atcttattga attctggtat gtcttaaagg attagttcac
     4801 ttcccaaatc aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa
     4861 tgaagaaatt gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta
     4921 gtttaaaatt ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg
     4981 aataacagtc ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt
     5041 tatttttaaa tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc
     5101 cttccttact ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg
     5161 gaacattatt attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg
     5221 cacagatgtg cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat
     5281 ttcatgatgc acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg
     5341 taagacccta aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt
     5401 ctatagtcca ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc
     5461 aatagttttc tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa
     5521 atttcatttt cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt
     5581 ggctagaaca ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat
     5641 ttgtttacag ggatcccatc gatttaaagc tatggagcaa aagctcattt ctgaagagga
     5701 cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa
     5761 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt
     5821 gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg
     5881 cgacctcacc atggaggagg gcggcaggtc gccccgggag gaggcggccg aaccgcagga
     5941 gtccggcggg gacgcggaac ccggcggagg gcgtcgggct ctgctgctgc cccccggcga
     6001 ccccccgcac ccccacccgc accctcaccg catcaccaac ttcttcatcg acaacatcct
     6061 gaggcccgag ttcggtcgga ggaaggaagc gggaggaacc gccggagaac cccgcaggcc
     6121 cggagcggag agcaggagga gccccgcggc cgccgcgccc gctcccggag ctccggtgcc
     6181 cggcggagga ggcggcggcg ggggtggatc gccgggccga ggggagggcg gccccgccgc
     6241 tctggccctg cacggagcgg ccaagaaggg gggagacccc gcggcgctgg aggcggccct
     6301 gaaagcgcgg gggctgagcg gcgcggagct gtcggtggaa gaggacgagg atgaagaaca
     6361 ggccggatcc aacgccggga accaacccat gctttggccc gcttgggtgt actgcacgcg
     6421 gtactcggac cgaccgtctt caggtccccg ctcccgcaaa ccaaagaaga agaaccccaa
     6481 caaggaagac aagcggcccc gcaccgcctt cacggccgag cagctgcaga gactcaaggc
     6541 cgagttccag acgaaccgct acctgacgga gcagcgccgg cagagcctgg cccaggagct
     6601 cggcctcaac gagtcccaga tcaagatctg gttccagaac aagagagcca agatcaagaa
     6661 ggcgacgggc agcaagaact cgctggcggt gcacctcatg gcccaggggc tctacaacca
     6721 ctccaccacg gcgaaagacg gcaagtcgga cagcgaagct ttggcgcccg ggccagagaa
     6781 cctgtacttc caggggctcg agccctctgc cggagacatg agagctgcaa acctttggcc
     6841 gagcccgctg atgattaaac gctctaagaa gaacagcctg gccttgtccc tgaccgccga
     6901 ccagatggtc agtgccttgt tggatgccga gccccccata ctctattccg agtatgatcc
     6961 taccagaccc ttcagtgagg cttcgatgat gggcttactg accaacctgg cagacaggga
     7021 gctggttcac atgattaact gggccaagag ggtgccaggc tttgtggatt tgaccctcca
     7081 cgatcaggtc cacctcctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg
     7141 gcgcagcatg gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa
     7201 ccagggaaaa tgtgtagagg gcatggtgga gattttcgac atgctgctgg ctacatcatc
     7261 tcggttccgc atgatgaatc tgcagggaga ggaatttgtg tgcctcaaat ctattatttt
     7321 gcttaattct ggagtttaca catttctgtc cagcaccctg aagtctctgg aggaaaagga
     7381 ccatatccat cgagtgctgg acaagatcac agacactttg attcacctga tggctaaggc
     7441 tggcctgacc ctgcagcagc agcatcagag actcgcacag cttctcctga tcctctccca
     7501 catcaggcac atgagtaaca aaggcatgga gcatctgtac tctatgaagt gcaagaacgt
     7561 ggtgcccctc tatgatctgc tgctggaggc ggcggacgct caccgcctac atgcgcccac
     7621 tagccgtgga ggggcatccg tggaggaaac agaccaaagc cacttggcta ctgcgggctc
     7681 tacttcatct cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc
     7741 cacagcacca ggttccggca gcgctacaaa cttctctctg ctcaagcagg ctggagatgt
     7801 ggaagaaaac cctgggcccg aagacggcat ggtgagcaag ggcgaggagg ataacatggc
     7861 tatcatcaag gagttcatgc gcttcaaggt tcacatggag ggctccgtga acggccacga
     7921 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     7981 gaaggtgaca aagggcggcc ccctgccctt cgcctgggac atcctgagcc ctcagttcat
     8041 gtacggctcc aaagcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     8101 tttcccagag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     8161 agtgacccag gactcttctc tgcaggacgg cgagtttatc tacaaggtga agctgcgcgg
     8221 caccaacttc ccctccgacg gccccgtaat gcagaagaaa actatgggat gggaggcttc
     8281 ttccgagcgg atgtacccag aggacggagc cctgaagggc gagatcaagc agaggctgaa
     8341 gctgaaggac ggcggccact acgatgccga ggtcaagacc acctacaagg ccaagaagcc
     8401 cgtgcaactg cccggagcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     8461 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccacagca ccggcggcat
     8521 ggacgagctg tacaagtaag gcgcgccgat atctacgtag acatgataag atacattgat
     8581 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     8641 gatgctattg ctttatttgt aaccatatta agctgcaata aacaagttaa caacaacaat
     8701 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc ctgcaggact
     8761 ctagtcgcgg ccgctcttcc gctaatactc aagtacaatt ttaatggagt acttttttac
     8821 ttttactcaa gtaagattct agccagatac ttttactttt aattgagtaa aattttccct
     8881 aagtacttgt actttcactt gagtaaaatt tttgagtact ttttacacct ctgccctgca
     8941 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9001 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9061 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9121 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9181 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9241 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9301 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9361 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9421 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9481 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9541 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9601 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9661 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9721 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9781 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9841 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9901 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9961 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10021 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10081 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10141 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10201 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10261 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10321 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10381 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10441 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10501 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10561 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10621 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10681 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10741 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10801 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10921 t
//
