LOCUS       sequence-363980-       13529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of human NOTCH1 intracellular domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Gastfriend BD, Snyder ME, Holt HE, Daneman R, Palecek SP, Shusta EV
  TITLE     Notch3 directs differentiation of brain mural cells from human 
            pluripotent stem cell-derived neural crest.
  JOURNAL   Sci Adv. 2024 Feb 2;10(5):eadi1737. doi: 10.1126/sciadv.adi1737. 
            Epub 2024 Feb 2.
  PUBMED    38306433
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-2"; volume: "10(5):eadi1737. doi"; pages: " 
            10.1126/sciadv.adi1737. Epub 2024 Feb 2"
COMMENT     Sequence Label: pWPI-N1ICD
FEATURES             Location/Qualifiers
     source          1..13529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2144..3322
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2375..3313
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3270..3290
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3350..3359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5796..5913
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5962..6529
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5980..6531
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6124..6141)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6351..6370
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6534..6536,6537..6539,6540..7250)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6534..6536,6537..6539,6540..7250)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6579..6600)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6840..6859)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7187..7208
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7329..7345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7330..7346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7359..7947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7412..7432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7830..7841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8091..8124)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8144..8324
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8349..8367)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8350..8367)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8657..8676)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8890..8909)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9009..9031
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9069..9087)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9155..9259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9260..9328,9329..10120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9478..9497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10291..10879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10780..10799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11033..11050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11120..11140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11125..11454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11305..11440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12588..12653
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12783..12803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13228..13362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13265..13284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13319..13338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgt cgacgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
     2101 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     2161 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2221 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2281 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2341 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2401 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2461 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2521 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2581 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2641 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2701 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2761 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2821 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2881 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2941 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3001 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3061 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3121 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3181 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3241 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3301 tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaag ccaccatggt
     3361 gctgctgtcc cgcaagcgcc ggcggcagca tggccagctc tggttccctg agggcttcaa
     3421 agtgtctgag gccagcaaga agaagcggcg ggagcccctc ggcgaggact ccgtgggcct
     3481 caagcccctg aagaacgctt cagacggtgc cctcatggac gacaaccaga atgagtgggg
     3541 ggacgaggac ctggagacca agaagttccg gttcgaggag cccgtggttc tgcctgacct
     3601 ggacgaccag acagaccacc ggcagtggac tcagcagcac ctggatgccg ctgacctgcg
     3661 catgtctgcc atggccccca caccgcccca gggtgaggtt gacgccgact gcatggacgt
     3721 caatgtccgc gggcctgatg gcttcacccc gctcatgatc gcctcctgca gcgggggcgg
     3781 cctggagacg ggcaacagcg aggaagagga ggacgcgccg gccgtcatct ccgacttcat
     3841 ctaccagggc gccagcctgc acaaccagac agaccgcacg ggcgagaccg ccttgcacct
     3901 ggccgcccgc tactcacgct ctgatgccgc caagcgcctg ctggaggcca gcgcagatgc
     3961 caacatccag gacaacatgg gccgcacccc gctgcatgcg gctgtgtctg ccgacgcaca
     4021 aggtgtcttc cagatcctga tccggaaccg agccacagac ctggatgccc gcatgcatga
     4081 tggcacgacg ccactgatcc tggctgcccg cctggccgtg gagggcatgc tggaggacct
     4141 catcaactca cacgccgacg tcaacgccgt agatgacctg ggcaagtccg ccctgcactg
     4201 ggccgccgcc gtgaacaatg tggatgccgc agttgtgctc ctgaagaacg gggctaacaa
     4261 agatatgcag aacaacaggg aggagacacc cctgtttctg gccgcccggg agggcagcta
     4321 cgagaccgcc aaggtgctgc tggaccactt tgccaaccgg gacatcacgg atcatatgga
     4381 ccgcctgccg cgcgacatcg cacaggagcg catgcatcac gacatcgtga ggctgctgga
     4441 cgagtacaac ctggtgcgca gcccgcagct gcacggagcc ccgctggggg gcacgcccac
     4501 cctgtcgccc ccgctctgct cgcccaacgg ctacctgggc agcctcaagc ccggcgtgca
     4561 gggcaagaag gtccgcaagc ccagcagcaa aggcctggcc tgtggaagca aggaggccaa
     4621 ggacctcaag gcacggagga agaagtccca ggatggcaag ggctgcctgc tggacagctc
     4681 cggcatgctc tcgcccgtgg actccctgga gtcaccccat ggctacctgt cagacgtggc
     4741 ctcgccgcca ctgctgccct ccccgttcca gcagtctccg tccgtgcccc tcaaccacct
     4801 gcctgggatg cccgacaccc acctgggcat cgggcacctg aacgtggcgg ccaagcccga
     4861 gatggcggcg ctgggtgggg gcggccggct ggcctttgag actggcccac ctcgtctctc
     4921 ccacctgcct gtggcctctg gcaccagcac cgtcctgggc tccagcagcg gaggggccct
     4981 gaatttcact gtgggcgggt ccaccagttt gaatggtcaa tgcgagtggc tgtcccggct
     5041 gcagagcggc atggtgccga accaatacaa ccctctgcgg gggagtgtgg caccaggccc
     5101 cctgagcaca caggccccct ccctgcagca tggcatggta ggcccgctgc acagtagcct
     5161 tgctgccagc gccctgtccc agatgatgag ctaccagggc ctgcccagca cccggctggc
     5221 cacccagcct cacctggtgc agacccagca ggtgcagcca caaaacttac agatgcagca
     5281 gcagaacctg cagccagcaa acatccagca gcagcaaagc ctgcagccgc caccaccacc
     5341 accacagccg caccttggcg tgagctcagc agccagcggc cacctgggcc ggagcttcct
     5401 gagtggagag ccgagccagg cagacgtgca gccactgggc cccagcagcc tggcggtgca
     5461 cactattctg ccccaggaga gccccgccct gcccacgtcg ctgccatcct cgctggtccc
     5521 acccgtgacc gcagcccagt tcctgacgcc cccctcgcag cacagctact cctcgcctgt
     5581 ggacaacacc cccagccacc agctacaggt gcctgagcac cccttcctca ccccgtcccc
     5641 tgagtcccct gaccagtggt ccagctcgtc cccgcattcc aacgtctccg actggtccga
     5701 gggcgtctcc agccctccca ccagcatgca gtcccagatc gcccgcattc cggaggcctt
     5761 caagtaatta attaatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg
     5821 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5881 ttacaaaaac aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgaggtt
     5941 taaactacgg gctgcaggaa ttccgncccc ccccccctaa cgttactggc cgaagccgct
     6001 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     6061 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     6121 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     6181 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     6241 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     6301 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     6361 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     6421 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg
     6481 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat accatggtga
     6541 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     6601 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     6661 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     6721 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     6781 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     6841 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6901 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6961 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     7021 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     7081 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     7141 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     7201 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     7261 gatctcgact agctagtagc tagctagcta gtcgagctca agcttcgaat tcgatatcaa
     7321 gcttatcgcg ataccgtcga cctcgaggga attccgataa tcaacctctg gattacaaaa
     7381 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7441 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7501 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7561 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7621 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7681 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7741 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     7801 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7861 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7921 ggatctccct ttgggccgcc tccccgcatc gggaattcga gctcggtacc tttaagacca
     7981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8041 gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt
     8101 atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga
     8161 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8221 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8281 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     8341 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     8401 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     8461 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     8521 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     8581 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     8641 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     8701 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     8761 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     8821 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     8881 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     8941 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     9001 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     9061 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     9121 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9181 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9241 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     9301 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9361 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9421 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9481 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9541 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9601 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9661 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9721 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9781 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9841 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9901 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9961 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10021 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    10081 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    10141 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10201 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10261 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10321 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10381 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10441 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10501 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10561 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10621 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10681 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10741 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10801 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10861 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10921 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10981 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    11041 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    11101 gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    11161 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    11221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    11281 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    11341 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    11401 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
    11461 ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag
    11521 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
    11581 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
    11641 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
    11701 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
    11761 ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
    11821 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
    11881 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
    11941 gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
    12001 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa
    12061 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
    12121 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
    12181 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
    12241 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
    12301 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
    12361 tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat
    12421 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
    12481 tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg
    12541 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
    12601 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
    12661 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
    12721 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
    12781 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
    12841 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
    12901 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
    12961 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
    13021 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
    13081 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
    13141 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    13201 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    13261 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    13321 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
    13381 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
    13441 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
    13501 tgttaaatat gtactacaaa cttagtagt
//
