LOCUS       sequence-363987-       10051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible CRE activation in zebrafish permanent transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Sinha DK, Neveu P, Gagey N, Aujard I, Le Saux T, Rampon C, Gauron C,
            Kawakami K, Leucht C, Bally-Cuif L, Volovitch M, Bensimon D, Jullien
            L, Vriz S
  TITLE     Photoactivation of the CreER T2 recombinase for conditional 
            site-specific recombination with high spatiotemporal resolution.
  JOURNAL   Zebrafish. 2010 Jun;7(2):199-204. doi: 10.1089/zeb.2009.0632.
  PUBMED    20441524
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/zeb.2009"; 
            journalName: "Zebrafish"; date: "2010-06"; volume: "7"; issue: "2"; 
            pages: "199-204"
COMMENT     Sequence Label: pT22iU66CriT(#387)
FEATURES             Location/Qualifiers
     source          1..10051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(152..172)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(152..170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(181..192,193..380)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    431..552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(572..1279,1280..1282,1283..1285))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(611..632)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     960..979
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1219..1240
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2046..2057)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        5692..5710
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        5755..5773
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      5796..5805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6849..6869
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7842..7963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8083..8220,8221..8232)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(8304..8892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8384..8403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9063..9854,9855..9923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9686..9705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9924..10028)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc ccgatactcc agcttttgtt ccctttagtg agggttaatt gcgcgttaca
      181 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
      241 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
      301 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
      361 ttattttttg gagatcactt cctcataggg ataacagggt aatggccggc cttttcagga
      421 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      481 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      541 aataaacaag ttgcgtgcgc atttatttaa ggtacagctc gtccatgccg agagtgatcc
      601 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      661 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      721 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      781 ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt
      841 ggctgatgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      901 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      961 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     1021 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     1081 cgccccaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     1141 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     1201 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     1261 acagctcctc gcccttgctc accatgtcgg ctggcgaccg gtggatcatc ccccgcaagt
     1321 ctgactgagt ggaagtttca gttcaatgga agggagaaaa gagccagcct ttatatatgt
     1381 catgtattgc tgctgtctgc atgcgatgaa cctagagctt actttttctg tcaagtaagg
     1441 caaaagcaga gaacctttgt cttgcattgt gtttgatgtt gtgctgtgac agtcatttct
     1501 tagttcatgc tggtactttt gttccatagc tttacccaaa gagttatcca gcattcccag
     1561 ttattccaaa aactcaatgt cacaaatttt ccagtccatt gcttagcagc gttgaccccg
     1621 gagaccctga tgtggtaatt tacagtgcaa ttcagttgct ggtccaaaca ctttgtaaca
     1681 gtttaaagaa ttgtggttac tagtaaatcc ttaatttggc atcatctagg tgtcattctt
     1741 tgccactttt gaatcacaca aatgaaccaa acaatggtta gctagcatca aacatttacc
     1801 aaataatctc atcagtgtca ccccaatgtt taagaagcca cattttatgt ccccagatat
     1861 attaatatta ttttatatat aatttaaaat agtaatgtgg tgttactgta tttgtcatat
     1921 aacgcaatat atacagtatg tctttatctg tatcaatttt gtggtcacac catcaataaa
     1981 ctccagttta attatttaaa attgggtagt gataaccata taaaggaaca gtggctagtt
     2041 ccatgttgta gctcccaacc tgctttcagg tgatccatgc agtgtcgtat aaaaaggtaa
     2101 caatttttaa ccttggaaag caagtacact taaggtccca gatctgaagc tacccctaac
     2161 acactattct acacacagaa aatgctcttc actaggtcga gaccagcaaa gttctagaat
     2221 ttgtcgaaac atttatgtta tatatttcct gaaaaaaatt ctgagtaagt tcttaagtgt
     2281 tattgccagc aacataaaca acagacggca aaatgaataa atgataacaa agcagtaggc
     2341 ttaaataaac ctaattttta taggctgttc tctacaaccc tcaaacagtg attagttttg
     2401 tacttataaa cttgcccttt cattcatatt tcaagaaaat tggttcagaa gatctggata
     2461 ttctagcagt tgttcaagct catggaggga tcagtgacct gattccacaa tgactaggcc
     2521 taatccagaa attagatgac tgtcaacata aaaaggcaca gcactcacta gctgccctat
     2581 atattttatt atattttaca tatattattt tatttattta gctctgagtg ctgtactttc
     2641 tggttaaaga aaactgctta caacagctaa cctgtactac ctcaggctca gggaatttgg
     2701 aacaggtttg tctggtttgt ttctttaacc atgcatgctt gttttcaact atggcaacac
     2761 agtcacatgg gacattacag aaatgatttg tcgatgacat gcgacttttc tttaataaag
     2821 cgcaaagatc ccaaaaagca aacttttaac aaaaatcata taattatatt ttcaatccag
     2881 ctttgtagca actttgtgct gctgttcact cagcaacaga tagtcagtat aaggtcagtg
     2941 tgtctcaaag cagtgccatc tgtttcacac attgcgttct atatataagt gtgctggttg
     3001 acacgacact gtataaggcc taggctaaaa cacaaacaat gtagaatgac actgtgtttt
     3061 ttttgtaaac aaatgttgtt tttggttaaa catctttgtg aaaacatcct cctgtcatgt
     3121 atttgctata ttcaaatgtt aaacccgtgc agaatagaac atatacaaaa aaaaacaaca
     3181 caacacattt ttaaacatta ttaaatatca agtattgctg gcagttctgt ttctgtttta
     3241 cagtaccctt tgccacagtt ctccgctttt cctggtccag attccacaag tctgattcac
     3301 caatagcaaa gcgaataaac aaccaaagca gccaatcact gcttgtagac tgtcctgcga
     3361 gaccggccca ttccagcaca ttctggaaac ttcctttata tgataattat aaatacattt
     3421 aaattattga tacaaaacat gtaattccta gaacataacc atagcaatca ttagttttca
     3481 gggtaattat gtatttttag gatttgactg cggaaagatc tggtcatgtg acgtctcatg
     3541 aacgtcacgg ccctgggttt ctataaatac agtaggactc tcgaccatcg gcagattttt
     3601 cgaagaagaa gatcagtttc aggagccgta ctgttccgtt tcaacgcaaa tattaacggt
     3661 aagagcgaat ttcctagttt gttttcatgc cattctttaa aaccatagcg tattacttta
     3721 attatagtaa actttcgctt tctttattac aagagacgtt ttgtgttgat tctccgcgga
     3781 cattttcggt cagacaatca gaaaatgacc gcggaggacc agtaacttgc attacacgta
     3841 agttaaatct tcgtgtatta aaatggttag gttgttaacg tcaaataggt taccgtgttt
     3901 gcgtgtgatc aggttggttt tgttagattt ttgtcagtat ttttaattta tttgttttag
     3961 tttatttatt ttttttgctg aatcatagtt tgtgaacaaa gaacccggat gttacataca
     4021 gtacagccgc catgttacag agagttataa cttaatcatt ttaaaaataa ttttgcctta
     4081 cttttagttt gtcatgttga gaaatgagga aatgttaaaa tgaggaaata tccaattaat
     4141 ttaatatatc aaaataatcc atgattacaa tgcactgaac tggagaaaat taagatgttt
     4201 tctagtgtca tgaaacaaat gtaagagatg tacattgtag atgttttatg tcaagaattg
     4261 gctagttgat gcagcatact ggcgatactc agttgtaata acagtaacgt tacatgttaa
     4321 tagactactg agtatgctgt tctgtctatg tatgctctgt aagctacgag aaggactttt
     4381 ttaaacagta aagggtgcaa tatttttaca aattgaatta aataaaggct gtctattaag
     4441 taatatgctt gatatttttc ttacttgatc ggaaataaga aaaaatataa acgttgttgc
     4501 tctaaaaatc ctagttcagt ttagccaacc acaaatacct ttttgttcct ccaacagttt
     4561 ttttttcttc tctataatat ttggcagtct atagtactcc aaatgtttcc ccacagtcta
     4621 actaattggt acagccaaaa tcatgacact tattgcaata ataattttgg ttcattggca
     4681 ttgttgatag cctgtgccac taatatggtc gattgatcat gcttcaggaa gaaaactata
     4741 ttgtttgatg taagattatt aaatcttcac ctgcttccat tacaaactat tcccatctta
     4801 ttgaattctg gtatgtctta aaggattagt tcacttccca aatcaaaatt tacttagcct
     4861 tttttcatcc atgatccctt tttttcatca ttaatgaaga aattgttttt gaaaaagttt
     4921 caagattttt ttctctatat tgtggagctt gttagtttaa aattccaaaa tgcaatatgt
     4981 ggcttcaaat ggttctaaat gatcccagtc aaggaataac agtcttatct aatgaaacca
     5041 ttagaccttt aaaaataaaa aaaataaaag tatttatttt taaatgactg agtgattaag
     5101 ttgaatttca gcgtttcctt actgtgtaga agtccttcct tactggcccc accctttggt
     5161 tctctgccaa tctgctacct aatgtaatgt tgtggaacat tattattctt tatttcttaa
     5221 ttttttattt tttattttaa aacaatgtaa actgcacaga tgtgcagttt gtttaaaatg
     5281 gccaatgctt tggaaatgca tgacataatt agatttcatg atgcacaaag ccaaatctca
     5341 gagcttgtgc aaaatgagct atcatttcac taggtaagac cctaaatttt catataggat
     5401 catttggaca attttgctgc aggtaaaatg cattctatag tccactgtca gccattgttt
     5461 tggatagtat ttatttttct ctacaagtat agtcaatagt tttctattat tttaaaggtt
     5521 tgtaacattt aagggtgacc aaatgcaaag taaaatttca ttttcgggtg aactatctcg
     5581 tttaacatgg gagaagtgca aaacatacat tattggctag aacattgtag tattttttaa
     5641 atggaaatgt gtgattgcta atcttacttt gaatttgttt acagggatct gatttaggtg
     5701 acactataga atacaagcta cttgttcttt ttgcaggatc acctgctttt catgatttag
     5761 gtgacactat agaatacaag ctacttgttc tttttgccac catggcttcc aatttactga
     5821 ccgtacacca aaatttgcct gctctgccgg tcgatgcaac gagtgatgag gttcgcaaga
     5881 acctgatgga tatgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc
     5941 tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg
     6001 cagaaccaga ggatgtgcgc gattatcttc tatatcttca ggctcgcggt ctggcagtaa
     6061 aaactatcca gcaacatttg ggccagctaa acatgcttca tagacgctct gggctgccac
     6121 gaccaagtga cagcaatgct gtttcactgg ttatgagaag gatcagaaaa gaaaacgttg
     6181 atgccggtga acgtgcaaaa caggctctag cgttcgagcg cactgacttc gatcaggtgc
     6241 gctctctgat ggaaaatagc gaccgctgcc aggacatccg caacctggca tttctgggga
     6301 ttgcttacaa caccctgctc agaatcgccg aaattgccag gatcagggtt aaagacatct
     6361 cacgcactga cggtggaaga atgttaatcc atattggcag aactaaaaca ctggttagca
     6421 ccgctggtgt agagaaggca cttagcctgg gagtaactaa actggtcgag agatggattt
     6481 ctgtctctgg tgtagctgat gatcctaata actacctgtt ttgcagggtc agaaaaaatg
     6541 gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg
     6601 aagcaactca cagattgatt tacggcgcta aggatgactc tggacagaga tacctggcct
     6661 ggtctggaca cagtgctcgc gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa
     6721 tccctgagat catgcaagct ggtggctgga ccaatgtgaa cattgtgatg aactatatcc
     6781 gcaacctgga tagtgaaaca ggagctatgg tgcgcctgct ggaagatggc gacctgaggc
     6841 ccgggccaga gaacctgtac ttccagggac tcgagccctc tgccggagac atgagagctg
     6901 caaacctttg gccgagcccg ctgatgatta aacgctctaa gaagaacagc ctggccttgt
     6961 ccctgaccgc cgaccagatg gtcagtgcct tgttggatgc cgagcccccc atactctatt
     7021 ccgagtatga tcctaccaga cccttcagtg aggcttcgat gatgggctta ctgaccaacc
     7081 tggcagacag ggagctggtt cacatgatta actgggccaa gagggtgcca ggctttgtgg
     7141 atttgaccct ccacgatcag gtccacctcc tagaatgtgc ctggctagag atcctgatga
     7201 ttggtctcgt ctggcgcagc atggagcacc cagtgaagct actgtttgct cctaacttgc
     7261 tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagattttc gacatgctgc
     7321 tggctacatc atctcggttc cgcatgatga atctgcaggg agaggaattt gtgtgcctca
     7381 aatctattat tttgcttaat tctggagttt acacatttct gtccagcacc ctgaagtctc
     7441 tggaggaaaa ggaccatatc catcgagtgc tggacaagat cacagacact ttgattcacc
     7501 tgatggctaa ggctggcctg accctgcagc agcagcatca gagactcgca cagcttctcc
     7561 tgatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg tactctatga
     7621 agtgcaagaa cgtggtgccc ctctatgatc tgctgctgga ggcggcggac gctcaccgcc
     7681 tacatgcgcc cactagccgt ggaggggcat ccgtggagga aacagaccaa agccacttgg
     7741 ctactgcggg ctctacttca tctcattcct tgcaaaagta ttacatcacg ggggaggcag
     7801 agggtttccc tgccacagca ccaggatcta cgtagacatg ataagataca ttgatgagtt
     7861 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7921 tattgcttta tttgtaacca tattaagctg caataaacaa gttaacaaca acaattgcat
     7981 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcctgca ggactctagt
     8041 cgcggccgct agggataaca gggtaatggc cgctcttccg ctaatactca agtacaattt
     8101 taatggagta cttttttact tttactcaag taagattcta gccagatact tttactttta
     8161 attgagtaaa attttcccta agtacttgta ctttcacttg agtaaaattt ttgagtactt
     8221 tttacacctc tgccctgcat cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8281 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8701 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9121 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10021 gttccgcgca catttccccg aaaagtgcca c
//
