LOCUS       sequence-363990-       10780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible chicken EN2 (wt) activation in zebrafish 
            permanent transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Rampon C, Gauron C, Lin T, Meda F, Dupont E, Cosson A, Ipendey E, 
            Frerot A, Aujard I, Le Saux T, Bensimon D, Jullien L, Volovitch M, 
            Vriz S, Joliot A
  TITLE     Control of brain patterning by Engrailed paracrine transfer: a new 
            function of the Pbx interaction domain.
  JOURNAL   Development. 2015 May 15;142(10):1840-9. doi: 10.1242/dev.114181. 
            Epub 2015 Apr 29.
  PUBMED    25926358
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.114181"; journalName: "Development"; date: "2015-05-15-
            15"; volume: "142"; issue: "10"; pages: "1840-9"
COMMENT     Sequence Label: pT22UbHEn2E4PCh(#262)
FEATURES             Location/Qualifiers
     source          1..10780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    397..518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(538..1245,1246..1248,1249..1251))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(577..598)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     926..945
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1185..1206
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2012..2023)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5660..5686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5690..5716
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5720..5746
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6635..6655
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7622..7678
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7688..8398
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8426..8547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8643..8780,8781..8792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8860..8877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9031..9619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9111..9130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9790..10581,10582..10650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10413..10432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10651..10755)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcaggaccag acatgataag atacattgat gagtttggac
      421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      481 ctttatttgt aaccattata agctgcaata aacaagttgc gtgcgcattt atttaaggta
      541 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      601 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      661 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      721 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      781 cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct tgtgccccag
      841 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      901 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      961 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1021 gtcggggtag cggctgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
     1081 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1141 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1201 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgtcggctgg
     1261 cgaccggtgg atcatccccc gcaagtctga ctgagtggaa gtttcagttc aatggaaggg
     1321 agaaaagagc cagcctttat atatgtcatg tattgctgct gtctgcatgc gatgaaccta
     1381 gagcttactt tttctgtcaa gtaaggcaaa agcagagaac ctttgtcttg cattgtgttt
     1441 gatgttgtgc tgtgacagtc atttcttagt tcatgctggt acttttgttc catagcttta
     1501 cccaaagagt tatccagcat tcccagttat tccaaaaact caatgtcaca aattttccag
     1561 tccattgctt agcagcgttg accccggaga ccctgatgtg gtaatttaca gtgcaattca
     1621 gttgctggtc caaacacttt gtaacagttt aaagaattgt ggttactagt aaatccttaa
     1681 tttggcatca tctaggtgtc attctttgcc acttttgaat cacacaaatg aaccaaacaa
     1741 tggttagcta gcatcaaaca tttaccaaat aatctcatca gtgtcacccc aatgtttaag
     1801 aagccacatt ttatgtcccc agatatatta atattatttt atatataatt taaaatagta
     1861 atgtggtgtt actgtatttg tcatataacg caatatatac agtatgtctt tatctgtatc
     1921 aattttgtgg tcacaccatc aataaactcc agtttaatta tttaaaattg ggtagtgata
     1981 accatataaa ggaacagtgg ctagttccat gttgtagctc ccaacctgct ttcaggtgat
     2041 ccatgcagtg tcgtataaaa aggtaacaat ttttaacctt ggaaagcaag tacacttaag
     2101 gtcccagatc tgaagctacc cctaacacac tattctacac acagaaaatg ctcttcacta
     2161 ggtcgagacc agcaaagttc tagaatttgt cgaaacattt atgttatata tttcctgaaa
     2221 aaaattctga gtaagttctt aagtgttatt gccagcaaca taaacaacag acggcaaaat
     2281 gaataaatga taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta
     2341 caaccctcaa acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa
     2401 gaaaattggt tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag
     2461 tgacctgatt ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa
     2521 ggcacagcac tcactagctg ccctatatat tttattatat tttacatata ttattttatt
     2581 tatttagctc tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg
     2641 tactacctca ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc
     2701 atgcttgttt tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga
     2761 tgacatgcga cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa
     2821 atcatataat tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc
     2881 aacagatagt cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg
     2941 cgttctatat ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca
     3001 aacaatgtag aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc
     3061 tttgtgaaaa catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa
     3121 tagaacatat acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta
     3181 ttgctggcag ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg
     3241 gtccagattc cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca
     3301 atcactgctt gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc
     3361 tttatatgat aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac
     3421 ataaccatag caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga
     3481 aagatctggt catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta
     3541 ggactctcga ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt
     3601 tccgtttcaa cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt
     3661 ctttaaaacc atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga
     3721 gacgttttgt gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg
     3781 aggaccagta acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg
     3841 ttaacgtcaa ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt
     3901 cagtattttt aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg
     3961 aacaaagaac ccggatgtta catacagtac agccgccatg ttacagagag ttataactta
     4021 atcattttaa aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg
     4081 ttaaaatgag gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca
     4141 ctgaactgga gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca
     4201 ttgtagatgt tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt
     4261 gtaataacag taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg
     4321 ctctgtaagc tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt
     4381 gaattaaata aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa
     4441 ataagaaaaa atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa
     4501 ataccttttt gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag
     4561 tactccaaat gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt
     4621 gcaataataa ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt
     4681 gatcatgctt caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc
     4741 ttccattaca aactattccc atcttattga attctggtat gtcttaaagg attagttcac
     4801 ttcccaaatc aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa
     4861 tgaagaaatt gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta
     4921 gtttaaaatt ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg
     4981 aataacagtc ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt
     5041 tatttttaaa tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc
     5101 cttccttact ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg
     5161 gaacattatt attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg
     5221 cacagatgtg cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat
     5281 ttcatgatgc acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg
     5341 taagacccta aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt
     5401 ctatagtcca ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc
     5461 aatagttttc tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa
     5521 atttcatttt cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt
     5581 ggctagaaca ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat
     5641 ttgtttacag ggatccatgt acccatacga tgtgcctgac tatgctggct acccttatga
     5701 cgttccagat tacgcaggct atccttacga cgttccagac tacgctccca tggaggaggg
     5761 cggcaggtcg ccccgggagg aggcggccga accgcaggag tccggcgggg acgcggaacc
     5821 cggcggaggg cgtcgggctc tgctgctgcc ccccggcgac cccccgcacc cccacccgca
     5881 ccctcaccgc atcaccaact tcttcatcga caacatcctg aggcccgagt tcggtcggag
     5941 gaaggaagcg ggaggaaccg ccggagaacc ccgcaggccc ggagcggaga gcaggaggag
     6001 ccccgcggcc gccgcgcccg ctcccggagc tccggtgccc ggcggaggag gcggcggcgg
     6061 gggtggatcg ccgggccgag gggagggcgg ccccgccgct ctggccctgc acggagcggc
     6121 caagaagggg ggagaccccg cggcgctgga ggcggccctg aaagcgcggg ggctgagcgg
     6181 cgcggagctg tcggtgagct cggactcgga tagctcccag gccggctcca acgccgggaa
     6241 ccaacccatg ctttggcccg cttgggtgta ctgcacgcgg tactcggacc gaccgtcttc
     6301 aggtccccgc tcccgcaaac caaagaagaa gaaccccaac aaggaagaca agcggccccg
     6361 caccgccttc acggccgagc agctgcagag actcaaggcc gagttccaga cgaaccgcta
     6421 cctgacggag cagcgccggc agagcctggc ccaggagctc ggcctcaacg agtcccagat
     6481 caagatctgg ttccagaaca agagagccaa gatcaagaag gcgacgggca gcaagaactc
     6541 gctggcggtg cacctcatgg cccaggggct ctacaaccac tccaccacgg cgaaagacgg
     6601 caagtcggac agcgaagctt tggcgcccgg gccagagaac ctgtacttcc aggggctcga
     6661 gccctctgcc ggagacatga gagctgcaaa cctttggccg agcccgctga tgattaaacg
     6721 ctctaagaag aacagcctgg ccttgtccct gaccgccgac cagatggtca gtgccttgtt
     6781 ggatgccgag ccccccatac tctattccga gtatgatcct accagaccct tcagtgaggc
     6841 ttcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca tgattaactg
     6901 ggccaagagg gtgccaggct ttgtggattt gaccctccac gatcaggtcc acctcctaga
     6961 atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg cgcagcatgg agcacccagt
     7021 gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat gtgtagaggg
     7081 catggtggag attttcgaca tgctgctggc tacatcatct cggttccgca tgatgaatct
     7141 gcagggagag gaatttgtgt gcctcaaatc tattattttg cttaattctg gagtttacac
     7201 atttctgtcc agcaccctga agtctctgga ggaaaaggac catatccatc gagtgctgga
     7261 caagatcaca gacactttga ttcacctgat ggctaaggct ggcctgaccc tgcagcagca
     7321 gcatcagaga ctcgcacagc ttctcctgat cctctcccac atcaggcaca tgagtaacaa
     7381 aggcatggag catctgtact ctatgaagtg caagaacgtg gtgcccctct atgatctgct
     7441 gctggaggcg gcggacgctc accgcctaca tgcgcccact agccgtggag gggcatccgt
     7501 ggaggaaaca gaccaaagcc acttggctac tgcgggctct acttcatctc attccttgca
     7561 aaagtattac atcacggggg aggcagaggg tttccctgcc acagcaccag gttccggcag
     7621 cgctacaaac ttctctctgc tcaagcaggc tggagatgtg gaagaaaacc ctgggcccga
     7681 agacggcatg gtgagcaagg gcgaggagga taacatggct atcatcaagg agttcatgcg
     7741 cttcaaggtt cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     7801 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacaa agggcggccc
     7861 cctgcccttc gcctgggaca tcctgagccc tcagttcatg tacggctcca aagcctacgt
     7921 gaagcacccc gccgacatcc ccgactactt gaagctgtct ttcccagagg gcttcaagtg
     7981 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgaca gtgacccagg actcttctct
     8041 gcaggacggc gagtttatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     8101 ccccgtaatg cagaagaaaa ctatgggatg ggaggcttct tccgagcgga tgtacccaga
     8161 ggacggagcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     8221 cgatgccgag gtcaagacca cctacaaggc caagaagccc gtgcaactgc ccggagccta
     8281 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     8341 gtacgaacgc gccgagggcc gccacagcac cggcggcatg gacgagctgt acaagtaagg
     8401 cgcgccgata tctacgtaga catgataaga tacattgatg agtttggaca aaccacaact
     8461 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     8521 accatattaa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     8581 gttcaggggg aggtgtggga ggttttttcc tgcaggactc tagtcgcggc cgctcttccg
     8641 ctaatactca agtacaattt taatggagta cttttttact tttactcaag taagattcta
     8701 gccagatact tttactttta attgagtaaa attttcccta agtacttgta ctttcacttg
     8761 agtaaaattt ttgagtactt tttacacctc tgccctgcat taatgaatcg gccaacgcgc
     8821 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8881 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8941 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9541 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9601 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9661 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9721 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9781 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9841 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9901 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9961 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10021 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10081 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10141 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10201 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10261 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10321 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10381 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10441 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10501 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10561 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10621 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10681 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10741 aataggggtt ccgcgcacat ttccccgaaa agtgccacct
//
