LOCUS       sequence-363997-       11893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible apoptosis activation and imaging (tEosFP) in 
            zebrafish permanent transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Feng Z, Zhang W, Xu J, Gauron C, Ducos B, Vriz S, Volovitch M, 
            Jullien L, Weiss S, Bensimon D
  TITLE     Optical control and study of biological processes at the single-cell
            level in a live organism.
  JOURNAL   Rep Prog Phys. 2013 Jul;76(7):072601. doi: 
            10.1088/0034-4885/76/7/072601. Epub 2013 Jun 14.
  PUBMED    23764902
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rep Prog 
            Phys."; date: "2013-07"; volume: "76(7):072601. doi"; pages: " 
            10.1088/0034-4885/76/7/072601. Epub 2013 Jun 14"
COMMENT     Sequence Label: pT22Apowriter2(#340)
FEATURES             Location/Qualifiers
     source          1..11893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(152..172)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(152..170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(181..192,193..380)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    399..520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(540..1247,1248..1250,1251..1253))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(579..600)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     928..947
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1187..1208
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2014..2025)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5662..5703
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             6184..6213
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6223..6252
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6262..6291
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6301..6330
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6340..6369
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6400..7080
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIA
                     RNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     CDS             7108..7164
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8548..8568
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    9541..9662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9758..9895,9896..9907)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9975..9992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10146..10734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10226..10245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10905..11696,11697..11765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11528..11547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11766..11870)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc ccgatactcc agcttttgtt ccctttagtg agggttaatt gcgcgttaca
      181 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
      241 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
      301 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
      361 ttattttttg gagatcactt cctcaggacc agacatgata agatacattg atgagtttgg
      421 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      481 tgctttattt gtaaccatta taagctgcaa taaacaagtt gcgtgcgcat ttatttaagg
      541 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      601 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      661 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      721 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc
      781 tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag cttgtgcccc
      841 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      901 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      961 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     1021 tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac gagggtgggc
     1081 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     1141 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     1201 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgtcggct
     1261 ggcgaccggt ggatcatccc ccgcaagtct gactgagtgg aagtttcagt tcaatggaag
     1321 ggagaaaaga gccagccttt atatatgtca tgtattgctg ctgtctgcat gcgatgaacc
     1381 tagagcttac tttttctgtc aagtaaggca aaagcagaga acctttgtct tgcattgtgt
     1441 ttgatgttgt gctgtgacag tcatttctta gttcatgctg gtacttttgt tccatagctt
     1501 tacccaaaga gttatccagc attcccagtt attccaaaaa ctcaatgtca caaattttcc
     1561 agtccattgc ttagcagcgt tgaccccgga gaccctgatg tggtaattta cagtgcaatt
     1621 cagttgctgg tccaaacact ttgtaacagt ttaaagaatt gtggttacta gtaaatcctt
     1681 aatttggcat catctaggtg tcattctttg ccacttttga atcacacaaa tgaaccaaac
     1741 aatggttagc tagcatcaaa catttaccaa ataatctcat cagtgtcacc ccaatgttta
     1801 agaagccaca ttttatgtcc ccagatatat taatattatt ttatatataa tttaaaatag
     1861 taatgtggtg ttactgtatt tgtcatataa cgcaatatat acagtatgtc tttatctgta
     1921 tcaattttgt ggtcacacca tcaataaact ccagtttaat tatttaaaat tgggtagtga
     1981 taaccatata aaggaacagt ggctagttcc atgttgtagc tcccaacctg ctttcaggtg
     2041 atccatgcag tgtcgtataa aaaggtaaca atttttaacc ttggaaagca agtacactta
     2101 aggtcccaga tctgaagcta cccctaacac actattctac acacagaaaa tgctcttcac
     2161 taggtcgaga ccagcaaagt tctagaattt gtcgaaacat ttatgttata tatttcctga
     2221 aaaaaattct gagtaagttc ttaagtgtta ttgccagcaa cataaacaac agacggcaaa
     2281 atgaataaat gataacaaag cagtaggctt aaataaacct aatttttata ggctgttctc
     2341 tacaaccctc aaacagtgat tagttttgta cttataaact tgccctttca ttcatatttc
     2401 aagaaaattg gttcagaaga tctggatatt ctagcagttg ttcaagctca tggagggatc
     2461 agtgacctga ttccacaatg actaggccta atccagaaat tagatgactg tcaacataaa
     2521 aaggcacagc actcactagc tgccctatat attttattat attttacata tattatttta
     2581 tttatttagc tctgagtgct gtactttctg gttaaagaaa actgcttaca acagctaacc
     2641 tgtactacct caggctcagg gaatttggaa caggtttgtc tggtttgttt ctttaaccat
     2701 gcatgcttgt tttcaactat ggcaacacag tcacatggga cattacagaa atgatttgtc
     2761 gatgacatgc gacttttctt taataaagcg caaagatccc aaaaagcaaa cttttaacaa
     2821 aaatcatata attatatttt caatccagct ttgtagcaac tttgtgctgc tgttcactca
     2881 gcaacagata gtcagtataa ggtcagtgtg tctcaaagca gtgccatctg tttcacacat
     2941 tgcgttctat atataagtgt gctggttgac acgacactgt ataaggccta ggctaaaaca
     3001 caaacaatgt agaatgacac tgtgtttttt ttgtaaacaa atgttgtttt tggttaaaca
     3061 tctttgtgaa aacatcctcc tgtcatgtat ttgctatatt caaatgttaa acccgtgcag
     3121 aatagaacat atacaaaaaa aaacaacaca acacattttt aaacattatt aaatatcaag
     3181 tattgctggc agttctgttt ctgttttaca gtaccctttg ccacagttct ccgcttttcc
     3241 tggtccagat tccacaagtc tgattcacca atagcaaagc gaataaacaa ccaaagcagc
     3301 caatcactgc ttgtagactg tcctgcgaga ccggcccatt ccagcacatt ctggaaactt
     3361 cctttatatg ataattataa atacatttaa attattgata caaaacatgt aattcctaga
     3421 acataaccat agcaatcatt agttttcagg gtaattatgt atttttagga tttgactgcg
     3481 gaaagatctg gtcatgtgac gtctcatgaa cgtcacggcc ctgggtttct ataaatacag
     3541 taggactctc gaccatcggc agatttttcg aagaagaaga tcagtttcag gagccgtact
     3601 gttccgtttc aacgcaaata ttaacggtaa gagcgaattt cctagtttgt tttcatgcca
     3661 ttctttaaaa ccatagcgta ttactttaat tatagtaaac tttcgctttc tttattacaa
     3721 gagacgtttt gtgttgattc tccgcggaca ttttcggtca gacaatcaga aaatgaccgc
     3781 ggaggaccag taacttgcat tacacgtaag ttaaatcttc gtgtattaaa atggttaggt
     3841 tgttaacgtc aaataggtta ccgtgtttgc gtgtgatcag gttggttttg ttagattttt
     3901 gtcagtattt ttaatttatt tgttttagtt tatttatttt ttttgctgaa tcatagtttg
     3961 tgaacaaaga acccggatgt tacatacagt acagccgcca tgttacagag agttataact
     4021 taatcatttt aaaaataatt ttgccttact tttagtttgt catgttgaga aatgaggaaa
     4081 tgttaaaatg aggaaatatc caattaattt aatatatcaa aataatccat gattacaatg
     4141 cactgaactg gagaaaatta agatgttttc tagtgtcatg aaacaaatgt aagagatgta
     4201 cattgtagat gttttatgtc aagaattggc tagttgatgc agcatactgg cgatactcag
     4261 ttgtaataac agtaacgtta catgttaata gactactgag tatgctgttc tgtctatgta
     4321 tgctctgtaa gctacgagaa ggactttttt aaacagtaaa gggtgcaata tttttacaaa
     4381 ttgaattaaa taaaggctgt ctattaagta atatgcttga tatttttctt acttgatcgg
     4441 aaataagaaa aaatataaac gttgttgctc taaaaatcct agttcagttt agccaaccac
     4501 aaataccttt ttgttcctcc aacagttttt ttttcttctc tataatattt ggcagtctat
     4561 agtactccaa atgtttcccc acagtctaac taattggtac agccaaaatc atgacactta
     4621 ttgcaataat aattttggtt cattggcatt gttgatagcc tgtgccacta atatggtcga
     4681 ttgatcatgc ttcaggaaga aaactatatt gtttgatgta agattattaa atcttcacct
     4741 gcttccatta caaactattc ccatcttatt gaattctggt atgtcttaaa ggattagttc
     4801 acttcccaaa tcaaaattta cttagccttt tttcatccat gatccctttt tttcatcatt
     4861 aatgaagaaa ttgtttttga aaaagtttca agattttttt ctctatattg tggagcttgt
     4921 tagtttaaaa ttccaaaatg caatatgtgg cttcaaatgg ttctaaatga tcccagtcaa
     4981 ggaataacag tcttatctaa tgaaaccatt agacctttaa aaataaaaaa aataaaagta
     5041 tttattttta aatgactgag tgattaagtt gaatttcagc gtttccttac tgtgtagaag
     5101 tccttcctta ctggccccac cctttggttc tctgccaatc tgctacctaa tgtaatgttg
     5161 tggaacatta ttattcttta tttcttaatt ttttattttt tattttaaaa caatgtaaac
     5221 tgcacagatg tgcagtttgt ttaaaatggc caatgctttg gaaatgcatg acataattag
     5281 atttcatgat gcacaaagcc aaatctcaga gcttgtgcaa aatgagctat catttcacta
     5341 ggtaagaccc taaattttca tataggatca tttggacaat tttgctgcag gtaaaatgca
     5401 ttctatagtc cactgtcagc cattgttttg gatagtattt atttttctct acaagtatag
     5461 tcaatagttt tctattattt taaaggtttg taacatttaa gggtgaccaa atgcaaagta
     5521 aaatttcatt ttcgggtgaa ctatctcgtt taacatggga gaagtgcaaa acatacatta
     5581 ttggctagaa cattgtagta ttttttaaat ggaaatgtgt gattgctaat cttactttga
     5641 atttgtttac agggatccat gatggggagt agcaagagca agcctaaaga ccctagccag
     5701 agaagcaggg gatctaccgg tgcatctgtt gtagctgatc ttccgtctta tggacctatc
     5761 gctttcgatc aggtggatgg cgtcacgaac gcgacccagc tattcaaaaa taatataaac
     5821 aaaaccagaa tgaacgattt aaaccgcgag gagacacgat taaagacctt caccgactgg
     5881 ccgctagatt ggctggataa acgccagctg gcccaaaccg gcatgtactt cacacacgct
     5941 ggcgacaaag ttaaatgctt tttctgcggc gtggaaatcg gttgctggga gcaggaggat
     6001 cagcccgtgc cggaacatca gcgatggtcg cccaactgtc cactgttgcg caggagaact
     6061 accaacaatg tgccgatcaa tgccgaagca ttagatcgca tcctgccgcc aataagctac
     6121 gacatctgcg gcgccaacga ctcgacgcta gagatgagga gatcccatcg atttaaagct
     6181 atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct
     6241 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
     6301 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
     6361 gaggacttga atgaaatgga gagcttgggc gacctcacca tggtgagtgc gattaagcca
     6421 gacatgaaga tcaacctccg tatggaaggc aacgtaaacg ggcaccattt tgtgatcgac
     6481 ggagatggta caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag
     6541 ggcggacctc tgccttttgc ctttgacatc ctgactaccg ctttccatta cggcaacagg
     6601 gtattcgccg aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaaagga
     6661 tattcgtggg aacgcagctt gactttcgag gacggaggca tttgtatcgc cagaaacgac
     6721 ataacaatgg aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc
     6781 gccaatggtc cagttatgca gaagaaaacg ctgaaatggg agccctccac tgagaaaatg
     6841 tatgtgcgtg atggagtgct gacgggtgat attacaatgg ctttgttgct tgaaggaaat
     6901 gcccattacc gatgtgactt cagaactact tacaaagcta aagagaaggg tgtcaagtta
     6961 ccaggctacc actttgttga ccactgtatt gagattttaa gccatgacaa agattacaac
     7021 aaggttaagc tgtatgagca cgctgttgct cattctggat tgcctgacaa tgccaggaga
     7081 tctcccaaga agaggaaggt gagatctgcc accaatttta gcctcctgaa acaagcaggc
     7141 gacgttgagg agaatccagg accgtctccc atgatggacg aggcggaccg ccagctcctg
     7201 agaagatgca gggtgcgcct agtgagcgag ctgcaagtcg cggagctgtg ggacgctctg
     7261 ctgagtcgag agctgttcac gcgcgacatg atcgaggata ttcagcaggc aggatctggc
     7321 tctcggcggg atcaggctag gcagctcgtc acagacctgg agactagagg ccgccaggct
     7381 ctgcctctgt tcatctcctg cttagaggac acaggccaag gcaccctggc ttcactcttg
     7441 caaagcggtc ggcaagcagc caagcaagac ccagaggctg ttaaacccct agaccatctg
     7501 gtgcctgtgg tcctgggacc aatgggactc acagcaaagg agcagagagt agtgaagctg
     7561 gacccgtcac agcctgccgt gggaaacctg accccagtgg tgctcggacc tgaagaactc
     7621 tggcctgctc gcctgaagcc agaggttctc agaccagaaa cacctagacc cgtggacatt
     7681 ggttctggtg gagcccatga tgtctgtgtt ccagggaaga tcaggggaca tgcagatatg
     7741 gcatacaccc tggactctga tccctgcggc cactgtctca tcatcaacaa tgtgaacttc
     7801 tgcccttcct cggggctcgg cacacgcacg ggctccaact tggaccgtga caaactggag
     7861 caccgattcc gctggctgcg cttcatggtg gaggtgaaga acgacctgac tgctaagaaa
     7921 atggtcacgg ctttgatgga gatggcacac cggaaccacc gtgccctgga ctgctttgtg
     7981 gtggtcatcc tctctcatgg ctgccaggcc agccacctcc agttcccagg tgctgtttat
     8041 gggacagatg gatgctccgt gtccattgag aaaattgtga acatctttaa cggaagcggc
     8101 tgccctagcc tgggagggaa gcccaaactg ttcttcatcc aggcttgcgg aggtgagcag
     8161 aaagaccacg gctttgaggt ggcttgcact tcctctcagg gcagaacctt ggacagtgac
     8221 tctgagcctg atgctgtccc ctatcaggaa ggcccaagac ccttggacca actggatgct
     8281 gtgtcaagtt tgcctacccc tagcgacatc cttgtgtcct actccacctt cccaggattt
     8341 gtgtcttgga gggacaagaa aagtggctcc tggtacatcg agactttgga cggtattctg
     8401 gaacagtggg ctcgctctga ggatctgcag tccctccttc tcagggttgc caatgctgtt
     8461 tctgcgaaag ggacttacaa gcagattcct ggctgtttta acttcctgcg gaaaaagctg
     8521 tttttcaaaa cttctgtgcc cgggccagag aacctgtact tccaggggct cgagccctct
     8581 gccggagaca tgagagctgc aaacctttgg ccgagcccgc tgatgattaa acgctctaag
     8641 aagaacagcc tggccttgtc cctgaccgcc gaccagatgg tcagtgcctt gttggatgcc
     8701 gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga ggcttcgatg
     8761 atgggcttac tgaccaacct ggcagacagg gagctggttc acatgattaa ctgggccaag
     8821 agggtgccag gctttgtgga tttgaccctc cacgatcagg tccacctcct agaatgtgcc
     8881 tggctagaga tcctgatgat tggtctcgtc tggcgcagca tggagcaccc agtgaagcta
     8941 ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg
     9001 gagattttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga
     9061 gaggaatttg tgtgcctcaa atctattatt ttgcttaatt ctggagttta cacatttctg
     9121 tccagcaccc tgaagtctct ggaggaaaag gaccatatcc atcgagtgct ggacaagatc
     9181 acagacactt tgattcacct gatggctaag gctggcctga ccctgcagca gcagcatcag
     9241 agactcgcac agcttctcct gatcctctcc cacatcaggc acatgagtaa caaaggcatg
     9301 gagcatctgt actctatgaa gtgcaagaac gtggtgcccc tctatgatct gctgctggag
     9361 gcggcggacg ctcaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggaa
     9421 acagaccaaa gccacttggc tactgcgggc tctacttcat ctcattcctt gcaaaagtat
     9481 tacatcacgg gggaggcaga gggtttccct gccacagcac caggatctac gtagacatga
     9541 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     9601 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat attaagctgc aataaacaag
     9661 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     9721 tttcctgcag gactctagtc gcggccgctc ttccgctaat actcaagtac aattttaatg
     9781 gagtactttt ttacttttac tcaagtaaga ttctagccag atacttttac ttttaattga
     9841 gtaaaatttt ccctaagtac ttgtactttc acttgagtaa aatttttgag tactttttac
     9901 acctctgccc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9961 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11881 cgaaaagtgc cac
//
