LOCUS       sequence-363999-        2401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Estradiol system inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 lexA-35S
FEATURES             Location/Qualifiers
     source          1..2401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      302..345
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(349..367)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             529..1188
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(595..614)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1209..1303
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1486..2074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1975..1994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2228..2245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2269..2326)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 atcctagagt cgagcatatt acatggcatt accaccatat acatatccat atacatatcc
       61 atatctaatc ttacctcgac tgctgtatat aaaaccagtg gttatatgta cagtactgct
      121 gtatataaaa ccagtggtta tatgtacagt acgtcgactg ctgtatataa aaccagtggt
      181 tatatgtaca gtactgctgt atataaaacc agtggttata tgtacagtac gtcgagggga
      241 tgataatgcg attagttttt tagcctcgac ctgcagccca agctccagag accgaattcc
      301 agaaatcatc cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac
      361 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactc
      421 gagttgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
      481 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      541 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      601 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      661 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      721 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
      781 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
      841 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
      901 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
      961 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1021 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1081 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     1141 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag
     1201 ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
     1261 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagcc tcgagctgtc
     1321 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1381 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1441 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1501 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1561 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1621 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1681 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1861 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2041 gtgatgctcg tcaggggggc ggagcctgtg gaaaaacgcc agcaacgcgg cctttttacg
     2101 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2161 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2221 cgagcgcagc gagtcagtga gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt
     2281 taaagaaaaa gggcagggtg gtgacacctt gccctttttt gccggactgc agggtctcac
     2341 attgctttat gactctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2401 g
//
