LOCUS       sequence-36400-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     5-series pFastBac Dual LIC vectors (for insect cell expression)
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac Dual His6 MBP N10 TEV LIC cloning vec...
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(228..248)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(306..329)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagtacttc
      121 tcgacaagct tgtcgagact gcaggctcta gattcgaaag cggccgcgac tagtgagctc
      181 gtcgacgtag gcctttgaat tcggatccgt tatccacttc caatattgga ttggaagtac
      241 aggttttcct cgatcccgag gttgttgtta ttgttattgt tgttgttgtt cgagctcgaa
      301 ttagtctgcg cgtctttcag ggcttcatcg acagtctgac gaccgctggc ggcgttgatc
      361 accgcagtac gcacggcata ccagaaagcg gacatctgcg ggatgttcgg catgatttca
      421 cctttctggg cgttttccat agtggcggca atacgtggat ctttcgccaa ctcttcctcg
      481 taagacttca gcgctacggc acccagcggt ttgtctttat taaccgcttc cagaccttca
      541 tcagtcagca gatagttttc gaggaactct tttgccagct ctttgttcgg actggcggcg
      601 ttaatacctg cgctcagcac gccaacgaac ggtttggatg gttgaccctt gaaggtcggc
      661 agtaccgtta caccataatt cactttgctg gtgtcgatgt tggaccatgc ccacgggccg
      721 ttgatggtca tcgctgtttc gcctttatta aaggcagctt ctgcgatgga gtaatcggtg
      781 tctgcattca tgtgtttgtt tttaatcagg tcaaccagga aggtcagacc cgctttcgcg
      841 ccagcgttat ccacgcccac gtctttaatg tcgtacttgc cgttttcata cttga
//
