LOCUS       sequence-364007-       10693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent
            transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Rampon C, Gauron C, Lin T, Meda F, Dupont E, Cosson A, Ipendey E, 
            Frerot A, Aujard I, Le Saux T, Bensimon D, Jullien L, Volovitch M, 
            Vriz S, Joliot A
  TITLE     Control of brain patterning by Engrailed paracrine transfer: a new 
            function of the Pbx interaction domain.
  JOURNAL   Development. 2015 May 15;142(10):1840-9. doi: 10.1242/dev.114181. 
            Epub 2015 Apr 29.
  PUBMED    25926358
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.114181"; journalName: "Development"; date: "2015-05-15-
            15"; volume: "142"; issue: "10"; pages: "1840-9"
COMMENT     Sequence Label: pT22UbhaEng2bE4PCh(#272)
FEATURES             Location/Qualifiers
     source          1..10693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    397..518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(538..1245,1246..1248,1249..1251))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(577..598)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     926..945
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1185..1206
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2012..2023)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5660..5686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5690..5716
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5720..5746
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6548..6568
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7535..7591
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7601..8311
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8339..8460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8556..8693,8694..8705)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8773..8790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8944..9532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9024..9043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9703..10494,10495..10563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10326..10345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10564..10668)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcaggaccag acatgataag atacattgat gagtttggac
      421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      481 ctttatttgt aaccattata agctgcaata aacaagttgc gtgcgcattt atttaaggta
      541 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      601 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      661 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      721 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      781 cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct tgtgccccag
      841 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      901 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      961 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1021 gtcggggtag cggctgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
     1081 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1141 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1201 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgtcggctgg
     1261 cgaccggtgg atcatccccc gcaagtctga ctgagtggaa gtttcagttc aatggaaggg
     1321 agaaaagagc cagcctttat atatgtcatg tattgctgct gtctgcatgc gatgaaccta
     1381 gagcttactt tttctgtcaa gtaaggcaaa agcagagaac ctttgtcttg cattgtgttt
     1441 gatgttgtgc tgtgacagtc atttcttagt tcatgctggt acttttgttc catagcttta
     1501 cccaaagagt tatccagcat tcccagttat tccaaaaact caatgtcaca aattttccag
     1561 tccattgctt agcagcgttg accccggaga ccctgatgtg gtaatttaca gtgcaattca
     1621 gttgctggtc caaacacttt gtaacagttt aaagaattgt ggttactagt aaatccttaa
     1681 tttggcatca tctaggtgtc attctttgcc acttttgaat cacacaaatg aaccaaacaa
     1741 tggttagcta gcatcaaaca tttaccaaat aatctcatca gtgtcacccc aatgtttaag
     1801 aagccacatt ttatgtcccc agatatatta atattatttt atatataatt taaaatagta
     1861 atgtggtgtt actgtatttg tcatataacg caatatatac agtatgtctt tatctgtatc
     1921 aattttgtgg tcacaccatc aataaactcc agtttaatta tttaaaattg ggtagtgata
     1981 accatataaa ggaacagtgg ctagttccat gttgtagctc ccaacctgct ttcaggtgat
     2041 ccatgcagtg tcgtataaaa aggtaacaat ttttaacctt ggaaagcaag tacacttaag
     2101 gtcccagatc tgaagctacc cctaacacac tattctacac acagaaaatg ctcttcacta
     2161 ggtcgagacc agcaaagttc tagaatttgt cgaaacattt atgttatata tttcctgaaa
     2221 aaaattctga gtaagttctt aagtgttatt gccagcaaca taaacaacag acggcaaaat
     2281 gaataaatga taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta
     2341 caaccctcaa acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa
     2401 gaaaattggt tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag
     2461 tgacctgatt ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa
     2521 ggcacagcac tcactagctg ccctatatat tttattatat tttacatata ttattttatt
     2581 tatttagctc tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg
     2641 tactacctca ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc
     2701 atgcttgttt tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga
     2761 tgacatgcga cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa
     2821 atcatataat tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc
     2881 aacagatagt cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg
     2941 cgttctatat ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca
     3001 aacaatgtag aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc
     3061 tttgtgaaaa catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa
     3121 tagaacatat acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta
     3181 ttgctggcag ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg
     3241 gtccagattc cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca
     3301 atcactgctt gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc
     3361 tttatatgat aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac
     3421 ataaccatag caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga
     3481 aagatctggt catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta
     3541 ggactctcga ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt
     3601 tccgtttcaa cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt
     3661 ctttaaaacc atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga
     3721 gacgttttgt gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg
     3781 aggaccagta acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg
     3841 ttaacgtcaa ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt
     3901 cagtattttt aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg
     3961 aacaaagaac ccggatgtta catacagtac agccgccatg ttacagagag ttataactta
     4021 atcattttaa aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg
     4081 ttaaaatgag gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca
     4141 ctgaactgga gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca
     4201 ttgtagatgt tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt
     4261 gtaataacag taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg
     4321 ctctgtaagc tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt
     4381 gaattaaata aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa
     4441 ataagaaaaa atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa
     4501 ataccttttt gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag
     4561 tactccaaat gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt
     4621 gcaataataa ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt
     4681 gatcatgctt caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc
     4741 ttccattaca aactattccc atcttattga attctggtat gtcttaaagg attagttcac
     4801 ttcccaaatc aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa
     4861 tgaagaaatt gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta
     4921 gtttaaaatt ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg
     4981 aataacagtc ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt
     5041 tatttttaaa tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc
     5101 cttccttact ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg
     5161 gaacattatt attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg
     5221 cacagatgtg cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat
     5281 ttcatgatgc acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg
     5341 taagacccta aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt
     5401 ctatagtcca ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc
     5461 aatagttttc tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa
     5521 atttcatttt cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt
     5581 ggctagaaca ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat
     5641 ttgtttacag ggatccatgt acccatacga tgtgcctgac tatgctggct acccttatga
     5701 cgttccagat tacgcaggct atccttacga cgttccagac tacgctccca tggaagaaaa
     5761 cgatcatagc aacagagatg tggagcgcca ggattcgggc gacgagtcca atagggctat
     5821 tcttcctctg ttgcaggctc ctggaaacgt actccctcac agaatcacca acttttacat
     5881 cgacaatatt ttaagaccag attttggccg caggaaagaa ggaagcagac gcgacgaaat
     5941 taacatagtt gagagagaga accgctgtcc atcggccccc ggatcaggac aggtagctcc
     6001 agtatcgggg gaaggaacat cgagccctcg cgctgtaaac gcatctaaaa aaactgatat
     6061 tagcacggac gaatctctga aatcccgtgc agagactgga gatcagtgtt taagttccga
     6121 ttcggactgc tcacaaagat gcgctgccca ggcgaaacag cctatgcttt ggcctgcttg
     6181 ggtatattgc acaagatatt cagacagacc ttcatctgga ccaagatcac gcaaaccaaa
     6241 gaagaaaacc ccaacaaagg aagacaagag accgagaaca gcattcacag cggagcaact
     6301 acagagactc aagaatgagt tccagaataa tcgttacctg acggagcaaa ggagacaagc
     6361 cctggcccag gaactcggcc tgaacgagtc tcagatcaaa atctggtttc aaaacaaaag
     6421 ggcaaagatc aaaaaagcaa cggggaacaa aaacacactt gccgtgcacc tgatggcaca
     6481 gggactttac aatcacgcca cagtaacaaa ggacgacaaa tcagacagtg atggactgcc
     6541 cgggccagag aacctgtact tccaggggct cgagccctct gccggagaca tgagagctgc
     6601 aaacctttgg ccgagcccgc tgatgattaa acgctctaag aagaacagcc tggccttgtc
     6661 cctgaccgcc gaccagatgg tcagtgcctt gttggatgcc gagcccccca tactctattc
     6721 cgagtatgat cctaccagac ccttcagtga ggcttcgatg atgggcttac tgaccaacct
     6781 ggcagacagg gagctggttc acatgattaa ctgggccaag agggtgccag gctttgtgga
     6841 tttgaccctc cacgatcagg tccacctcct agaatgtgcc tggctagaga tcctgatgat
     6901 tggtctcgtc tggcgcagca tggagcaccc agtgaagcta ctgtttgctc ctaacttgct
     6961 cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagattttcg acatgctgct
     7021 ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggaatttg tgtgcctcaa
     7081 atctattatt ttgcttaatt ctggagttta cacatttctg tccagcaccc tgaagtctct
     7141 ggaggaaaag gaccatatcc atcgagtgct ggacaagatc acagacactt tgattcacct
     7201 gatggctaag gctggcctga ccctgcagca gcagcatcag agactcgcac agcttctcct
     7261 gatcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt actctatgaa
     7321 gtgcaagaac gtggtgcccc tctatgatct gctgctggag gcggcggacg ctcaccgcct
     7381 acatgcgccc actagccgtg gaggggcatc cgtggaggaa acagaccaaa gccacttggc
     7441 tactgcgggc tctacttcat ctcattcctt gcaaaagtat tacatcacgg gggaggcaga
     7501 gggtttccct gccacagcac caggttccgg cagcgctaca aacttctctc tgctcaagca
     7561 ggctggagat gtggaagaaa accctgggcc cgaagacggc atggtgagca agggcgagga
     7621 ggataacatg gctatcatca aggagttcat gcgcttcaag gttcacatgg agggctccgt
     7681 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     7741 gaccgccaag ctgaaggtga caaagggcgg ccccctgccc ttcgcctggg acatcctgag
     7801 ccctcagttc atgtacggct ccaaagccta cgtgaagcac cccgccgaca tccccgacta
     7861 cttgaagctg tctttcccag agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     7921 cggcgtggtg acagtgaccc aggactcttc tctgcaggac ggcgagttta tctacaaggt
     7981 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga aaactatggg
     8041 atgggaggct tcttccgagc ggatgtaccc agaggacgga gccctgaagg gcgagatcaa
     8101 gcagaggctg aagctgaagg acggcggcca ctacgatgcc gaggtcaaga ccacctacaa
     8161 ggccaagaag cccgtgcaac tgcccggagc ctacaacgtc aacatcaagt tggacatcac
     8221 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccacag
     8281 caccggcggc atggacgagc tgtacaagta aggcgcgccg atatctacgt agacatgata
     8341 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     8401 tgtgaaattt gtgatgctat tgctttattt gtaaccatat taagctgcaa taaacaagtt
     8461 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     8521 tcctgcagga ctctagtcgc ggccgctctt ccgctaatac tcaagtacaa ttttaatgga
     8581 gtactttttt acttttactc aagtaagatt ctagccagat acttttactt ttaattgagt
     8641 aaaattttcc ctaagtactt gtactttcac ttgagtaaaa tttttgagta ctttttacac
     8701 ctctgccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8761 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8821 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8881 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8941 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9001 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9061 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9121 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9181 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9241 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9301 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9361 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9421 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9481 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9541 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9601 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9661 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9721 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9781 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9841 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9901 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9961 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10021 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10081 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10141 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10201 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10261 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10321 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10381 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10441 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10501 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10561 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10621 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10681 aaaagtgcca cct
//
