LOCUS       sequence-364008-       10705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent
            transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Rampon C, Gauron C, Lin T, Meda F, Dupont E, Cosson A, Ipendey E, 
            Frerot A, Aujard I, Le Saux T, Bensimon D, Jullien L, Volovitch M, 
            Vriz S, Joliot A
  TITLE     Control of brain patterning by Engrailed paracrine transfer: a new 
            function of the Pbx interaction domain.
  JOURNAL   Development. 2015 May 15;142(10):1840-9. doi: 10.1242/dev.114181. 
            Epub 2015 Apr 29.
  PUBMED    25926358
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.114181"; journalName: "Development"; date: "2015-05-15-
            15"; volume: "142"; issue: "10"; pages: "1840-9"
COMMENT     Sequence Label: pT22UbhaEng2aE4PCh(#271)
FEATURES             Location/Qualifiers
     source          1..10705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    397..518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(538..1245,1246..1248,1249..1251))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(577..598)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     926..945
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1185..1206
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2012..2023)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5660..5686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5690..5716
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5720..5746
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6560..6580
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7547..7603
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7613..8323
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8351..8472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8568..8705,8706..8717)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8785..8802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8956..9544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9036..9055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9715..10506,10507..10575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10338..10357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10576..10680)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcaggaccag acatgataag atacattgat gagtttggac
      421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      481 ctttatttgt aaccattata agctgcaata aacaagttgc gtgcgcattt atttaaggta
      541 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      601 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      661 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      721 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      781 cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct tgtgccccag
      841 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      901 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      961 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1021 gtcggggtag cggctgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
     1081 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1141 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1201 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgtcggctgg
     1261 cgaccggtgg atcatccccc gcaagtctga ctgagtggaa gtttcagttc aatggaaggg
     1321 agaaaagagc cagcctttat atatgtcatg tattgctgct gtctgcatgc gatgaaccta
     1381 gagcttactt tttctgtcaa gtaaggcaaa agcagagaac ctttgtcttg cattgtgttt
     1441 gatgttgtgc tgtgacagtc atttcttagt tcatgctggt acttttgttc catagcttta
     1501 cccaaagagt tatccagcat tcccagttat tccaaaaact caatgtcaca aattttccag
     1561 tccattgctt agcagcgttg accccggaga ccctgatgtg gtaatttaca gtgcaattca
     1621 gttgctggtc caaacacttt gtaacagttt aaagaattgt ggttactagt aaatccttaa
     1681 tttggcatca tctaggtgtc attctttgcc acttttgaat cacacaaatg aaccaaacaa
     1741 tggttagcta gcatcaaaca tttaccaaat aatctcatca gtgtcacccc aatgtttaag
     1801 aagccacatt ttatgtcccc agatatatta atattatttt atatataatt taaaatagta
     1861 atgtggtgtt actgtatttg tcatataacg caatatatac agtatgtctt tatctgtatc
     1921 aattttgtgg tcacaccatc aataaactcc agtttaatta tttaaaattg ggtagtgata
     1981 accatataaa ggaacagtgg ctagttccat gttgtagctc ccaacctgct ttcaggtgat
     2041 ccatgcagtg tcgtataaaa aggtaacaat ttttaacctt ggaaagcaag tacacttaag
     2101 gtcccagatc tgaagctacc cctaacacac tattctacac acagaaaatg ctcttcacta
     2161 ggtcgagacc agcaaagttc tagaatttgt cgaaacattt atgttatata tttcctgaaa
     2221 aaaattctga gtaagttctt aagtgttatt gccagcaaca taaacaacag acggcaaaat
     2281 gaataaatga taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta
     2341 caaccctcaa acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa
     2401 gaaaattggt tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag
     2461 tgacctgatt ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa
     2521 ggcacagcac tcactagctg ccctatatat tttattatat tttacatata ttattttatt
     2581 tatttagctc tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg
     2641 tactacctca ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc
     2701 atgcttgttt tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga
     2761 tgacatgcga cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa
     2821 atcatataat tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc
     2881 aacagatagt cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg
     2941 cgttctatat ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca
     3001 aacaatgtag aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc
     3061 tttgtgaaaa catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa
     3121 tagaacatat acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta
     3181 ttgctggcag ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg
     3241 gtccagattc cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca
     3301 atcactgctt gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc
     3361 tttatatgat aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac
     3421 ataaccatag caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga
     3481 aagatctggt catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta
     3541 ggactctcga ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt
     3601 tccgtttcaa cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt
     3661 ctttaaaacc atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga
     3721 gacgttttgt gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg
     3781 aggaccagta acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg
     3841 ttaacgtcaa ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt
     3901 cagtattttt aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg
     3961 aacaaagaac ccggatgtta catacagtac agccgccatg ttacagagag ttataactta
     4021 atcattttaa aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg
     4081 ttaaaatgag gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca
     4141 ctgaactgga gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca
     4201 ttgtagatgt tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt
     4261 gtaataacag taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg
     4321 ctctgtaagc tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt
     4381 gaattaaata aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa
     4441 ataagaaaaa atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa
     4501 ataccttttt gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag
     4561 tactccaaat gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt
     4621 gcaataataa ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt
     4681 gatcatgctt caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc
     4741 ttccattaca aactattccc atcttattga attctggtat gtcttaaagg attagttcac
     4801 ttcccaaatc aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa
     4861 tgaagaaatt gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta
     4921 gtttaaaatt ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg
     4981 aataacagtc ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt
     5041 tatttttaaa tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc
     5101 cttccttact ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg
     5161 gaacattatt attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg
     5221 cacagatgtg cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat
     5281 ttcatgatgc acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg
     5341 taagacccta aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt
     5401 ctatagtcca ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc
     5461 aatagttttc tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa
     5521 atttcatttt cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt
     5581 ggctagaaca ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat
     5641 ttgtttacag ggatccatgt acccatacga tgtgcctgac tatgctggct acccttatga
     5701 cgttccagat tacgcaggct atccttacga cgttccagac tacgctccca tggatgagaa
     5761 tgagcagagc gcaagagatg tggaacagcg cggagcgtcg gacgagtcca acagtgccat
     5821 acgacccctt ttacaagctc ctggaaacct ccaactgcca caccgaatca caaacttctt
     5881 catcgacaac atcctgcgac cagattttgg ccgtaaaaaa gaagcaaata tcacgcgcta
     5941 tgaggacaat cacggcgcac gagagaatca caaccctacg ggtccaagca cgggacaggt
     6001 gggaagcact gtaccagcgg aagaagcctc cacaacgcat acgagcagcg gaggaaagga
     6061 ggcagagata gagagcgaag aacccctgaa gcctcgcggg gagaatgtgg atcagtgctt
     6121 gggttcagaa tcggatagtt cacagagcaa ttcaaacgga cagactggtc agggtatgct
     6181 gtggcccgct tgggtttact gcacacgcta ctcggacagg ccgtcgtctg gtcccaggtc
     6241 tcgtaaacca aagaagaaag ccgcaagtaa agaagacaaa cgaccacgca cggccttcac
     6301 ggcggagcag cttcagagac tcaaggccga gttccagacc aaccgctacc tgaccgagca
     6361 gcggcggcaa agcctggcgc aggaactggg cctcaacgaa tctcagatca aaatctggtt
     6421 ccaaaacaag cgggccaaaa tcaaaaaggc cagcggagtc aagaacggtc tggcaataca
     6481 cctgatggca cagggactgt ataaccacag caccacatca aaggaggaca aatcagacag
     6541 tcacggactg cccgggccag agaacctgta cttccagggg ctcgagccct ctgccggaga
     6601 catgagagct gcaaaccttt ggccgagccc gctgatgatt aaacgctcta agaagaacag
     6661 cctggccttg tccctgaccg ccgaccagat ggtcagtgcc ttgttggatg ccgagccccc
     6721 catactctat tccgagtatg atcctaccag acccttcagt gaggcttcga tgatgggctt
     6781 actgaccaac ctggcagaca gggagctggt tcacatgatt aactgggcca agagggtgcc
     6841 aggctttgtg gatttgaccc tccacgatca ggtccacctc ctagaatgtg cctggctaga
     6901 gatcctgatg attggtctcg tctggcgcag catggagcac ccagtgaagc tactgtttgc
     6961 tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatttt
     7021 cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggaatt
     7081 tgtgtgcctc aaatctatta ttttgcttaa ttctggagtt tacacatttc tgtccagcac
     7141 cctgaagtct ctggaggaaa aggaccatat ccatcgagtg ctggacaaga tcacagacac
     7201 tttgattcac ctgatggcta aggctggcct gaccctgcag cagcagcatc agagactcgc
     7261 acagcttctc ctgatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct
     7321 gtactctatg aagtgcaaga acgtggtgcc cctctatgat ctgctgctgg aggcggcgga
     7381 cgctcaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg aaacagacca
     7441 aagccacttg gctactgcgg gctctacttc atctcattcc ttgcaaaagt attacatcac
     7501 gggggaggca gagggtttcc ctgccacagc accaggttcc ggcagcgcta caaacttctc
     7561 tctgctcaag caggctggag atgtggaaga aaaccctggg cccgaagacg gcatggtgag
     7621 caagggcgag gaggataaca tggctatcat caaggagttc atgcgcttca aggttcacat
     7681 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     7741 cgagggcacc cagaccgcca agctgaaggt gacaaagggc ggccccctgc ccttcgcctg
     7801 ggacatcctg agccctcagt tcatgtacgg ctccaaagcc tacgtgaagc accccgccga
     7861 catccccgac tacttgaagc tgtctttccc agagggcttc aagtgggagc gcgtgatgaa
     7921 cttcgaggac ggcggcgtgg tgacagtgac ccaggactct tctctgcagg acggcgagtt
     7981 tatctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     8041 gaaaactatg ggatgggagg cttcttccga gcggatgtac ccagaggacg gagccctgaa
     8101 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgatg ccgaggtcaa
     8161 gaccacctac aaggccaaga agcccgtgca actgcccgga gcctacaacg tcaacatcaa
     8221 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     8281 gggccgccac agcaccggcg gcatggacga gctgtacaag taaggcgcgc cgatatctac
     8341 gtagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     8401 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat attaagctgc
     8461 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     8521 tgggaggttt tttcctgcag gactctagtc gcggccgctc ttccgctaat actcaagtac
     8581 aattttaatg gagtactttt ttacttttac tcaagtaaga ttctagccag atacttttac
     8641 ttttaattga gtaaaatttt ccctaagtac ttgtactttc acttgagtaa aatttttgag
     8701 tactttttac acctctgccc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8761 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8821 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8881 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8941 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9001 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9061 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9121 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9181 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9241 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9301 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9361 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9421 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9481 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9541 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9601 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9661 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9721 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9781 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9841 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9901 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9961 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10021 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10081 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10141 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10201 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10261 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10321 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10381 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10441 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10501 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10561 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10621 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10681 cacatttccc cgaaaagtgc cacct
//
