LOCUS       sequence-364015-       10118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible apoptosis activation and imaging (tEosFP) by 
            zebrafish transient transgenesis or mRNA synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Feng Z, Zhang W, Xu J, Gauron C, Ducos B, Vriz S, Volovitch M, 
            Jullien L, Weiss S, Bensimon D
  TITLE     Optical control and study of biological processes at the single-cell
            level in a live organism.
  JOURNAL   Rep Prog Phys. 2013 Jul;76(7):072601. doi: 
            10.1088/0034-4885/76/7/072601. Epub 2013 Jun 14.
  PUBMED    23764902
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rep Prog 
            Phys."; date: "2013-07"; volume: "76(7):072601. doi"; pages: " 
            10.1088/0034-4885/76/7/072601. Epub 2013 Jun 14"
COMMENT     Sequence Label: pT2iU6Apowriter2N(#355)
FEATURES             Location/Qualifiers
     source          1..10118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        3975..3993
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3975..3992
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4028..4069
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             4550..4579
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4589..4618
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4628..4657
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4667..4696
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4706..4735
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4766..5446
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIA
                     RNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     CDS             5474..5530
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6914..6934
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7907..8028
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7918..7937)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7972..7991
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8148..8285,8286..8297)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(8369..8957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8449..8468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9128..9919,9920..9988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9751..9770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9989..10093)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcatagggat aacagggtaa tggccggcct tttttaggtc
      421 ccagatctga agctacccct aacacactat tctacacaca gaaaatgctc ttcactaggt
      481 cgagaccagc aaagttctag aatttgtcga aacatttatg ttatatattt cctgaaaaaa
      541 attctgagta agttcttaag tgttattgcc agcaacataa acaacagacg gcaaaatgaa
      601 taaatgataa caaagcagta ggcttaaata aacctaattt ttataggctg ttctctacaa
      661 ccctcaaaca gtgattagtt ttgtacttat aaacttgccc tttcattcat atttcaagaa
      721 aattggttca gaagatctgg atattctagc agttgttcaa gctcatggag ggatcagtga
      781 cctgattcca caatgactag gcctaatcca gaaattagat gactgtcaac ataaaaaggc
      841 acagcactca ctagctgccc tatatatttt attatatttt acatatatta ttttatttat
      901 ttagctctga gtgctgtact ttctggttaa agaaaactgc ttacaacagc taacctgtac
      961 tacctcaggc tcagggaatt tggaacaggt ttgtctggtt tgtttcttta accatgcatg
     1021 cttgttttca actatggcaa cacagtcaca tgggacatta cagaaatgat ttgtcgatga
     1081 catgcgactt ttctttaata aagcgcaaag atcccaaaaa gcaaactttt aacaaaaatc
     1141 atataattat attttcaatc cagctttgta gcaactttgt gctgctgttc actcagcaac
     1201 agatagtcag tataaggtca gtgtgtctca aagcagtgcc atctgtttca cacattgcgt
     1261 tctatatata agtgtgctgg ttgacacgac actgtataag gcctaggcta aaacacaaac
     1321 aatgtagaat gacactgtgt tttttttgta aacaaatgtt gtttttggtt aaacatcttt
     1381 gtgaaaacat cctcctgtca tgtatttgct atattcaaat gttaaacccg tgcagaatag
     1441 aacatataca aaaaaaaaca acacaacaca tttttaaaca ttattaaata tcaagtattg
     1501 ctggcagttc tgtttctgtt ttacagtacc ctttgccaca gttctccgct tttcctggtc
     1561 cagattccac aagtctgatt caccaatagc aaagcgaata aacaaccaaa gcagccaatc
     1621 actgcttgta gactgtcctg cgagaccggc ccattccagc acattctgga aacttccttt
     1681 atatgataat tataaataca tttaaattat tgatacaaaa catgtaattc ctagaacata
     1741 accatagcaa tcattagttt tcagggtaat tatgtatttt taggatttga ctgcggaaag
     1801 atctggtcat gtgacgtctc atgaacgtca cggccctggg tttctataaa tacagtagga
     1861 ctctcgacca tcggcagatt tttcgaagaa gaagatcagt ttcaggagcc gtactgttcc
     1921 gtttcaacgc aaatattaac ggtaagagcg aatttcctag tttgttttca tgccattctt
     1981 taaaaccata gcgtattact ttaattatag taaactttcg ctttctttat tacaagagac
     2041 gttttgtgtt gattctccgc ggacattttc ggtcagacaa tcagaaaatg accgcggagg
     2101 accagtaact tgcattacac gtaagttaaa tcttcgtgta ttaaaatggt taggttgtta
     2161 acgtcaaata ggttaccgtg tttgcgtgtg atcaggttgg ttttgttaga tttttgtcag
     2221 tatttttaat ttatttgttt tagtttattt attttttttg ctgaatcata gtttgtgaac
     2281 aaagaacccg gatgttacat acagtacagc cgccatgtta cagagagtta taacttaatc
     2341 attttaaaaa taattttgcc ttacttttag tttgtcatgt tgagaaatga ggaaatgtta
     2401 aaatgaggaa atatccaatt aatttaatat atcaaaataa tccatgatta caatgcactg
     2461 aactggagaa aattaagatg ttttctagtg tcatgaaaca aatgtaagag atgtacattg
     2521 tagatgtttt atgtcaagaa ttggctagtt gatgcagcat actggcgata ctcagttgta
     2581 ataacagtaa cgttacatgt taatagacta ctgagtatgc tgttctgtct atgtatgctc
     2641 tgtaagctac gagaaggact tttttaaaca gtaaagggtg caatattttt acaaattgaa
     2701 ttaaataaag gctgtctatt aagtaatatg cttgatattt ttcttacttg atcggaaata
     2761 agaaaaaata taaacgttgt tgctctaaaa atcctagttc agtttagcca accacaaata
     2821 cctttttgtt cctccaacag tttttttttc ttctctataa tatttggcag tctatagtac
     2881 tccaaatgtt tccccacagt ctaactaatt ggtacagcca aaatcatgac acttattgca
     2941 ataataattt tggttcattg gcattgttga tagcctgtgc cactaatatg gtcgattgat
     3001 catgcttcag gaagaaaact atattgtttg atgtaagatt attaaatctt cacctgcttc
     3061 cattacaaac tattcccatc ttattgaatt ctggtatgtc ttaaaggatt agttcacttc
     3121 ccaaatcaaa atttacttag ccttttttca tccatgatcc ctttttttca tcattaatga
     3181 agaaattgtt tttgaaaaag tttcaagatt tttttctcta tattgtggag cttgttagtt
     3241 taaaattcca aaatgcaata tgtggcttca aatggttcta aatgatccca gtcaaggaat
     3301 aacagtctta tctaatgaaa ccattagacc tttaaaaata aaaaaaataa aagtatttat
     3361 ttttaaatga ctgagtgatt aagttgaatt tcagcgtttc cttactgtgt agaagtcctt
     3421 ccttactggc cccacccttt ggttctctgc caatctgcta cctaatgtaa tgttgtggaa
     3481 cattattatt ctttatttct taatttttta ttttttattt taaaacaatg taaactgcac
     3541 agatgtgcag tttgtttaaa atggccaatg ctttggaaat gcatgacata attagatttc
     3601 atgatgcaca aagccaaatc tcagagcttg tgcaaaatga gctatcattt cactaggtaa
     3661 gaccctaaat tttcatatag gatcatttgg acaattttgc tgcaggtaaa atgcattcta
     3721 tagtccactg tcagccattg ttttggatag tatttatttt tctctacaag tatagtcaat
     3781 agttttctat tattttaaag gtttgtaaca tttaagggtg accaaatgca aagtaaaatt
     3841 tcattttcgg gtgaactatc tcgtttaaca tgggagaagt gcaaaacata cattattggc
     3901 tagaacattg tagtattttt taaatggaaa tgtgtgattg ctaatcttac tttgaatttg
     3961 tttacaggga tctgatttag gtgacactat agaatacaag ctacttgttc tttttgcagg
     4021 atccatgatg gggagtagca agagcaagcc taaagaccct agccagagaa gcaggggatc
     4081 taccggtgca tctgttgtag ctgatcttcc gtcttatgga cctatcgctt tcgatcaggt
     4141 ggatggcgtc acgaacgcga cccagctatt caaaaataat ataaacaaaa ccagaatgaa
     4201 cgatttaaac cgcgaggaga cacgattaaa gaccttcacc gactggccgc tagattggct
     4261 ggataaacgc cagctggccc aaaccggcat gtacttcaca cacgctggcg acaaagttaa
     4321 atgctttttc tgcggcgtgg aaatcggttg ctgggagcag gaggatcagc ccgtgccgga
     4381 acatcagcga tggtcgccca actgtccact gttgcgcagg agaactacca acaatgtgcc
     4441 gatcaatgcc gaagcattag atcgcatcct gccgccaata agctacgaca tctgcggcgc
     4501 caacgactcg acgctagaga tgaggagatc ccatcgattt aaagctatgg agcaaaagct
     4561 catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa
     4621 tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat
     4681 ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga
     4741 aatggagagc ttgggcgacc tcaccatggt gagtgcgatt aagccagaca tgaagatcaa
     4801 cctccgtatg gaaggcaacg taaacgggca ccattttgtg atcgacggag atggtacagg
     4861 caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc
     4921 ttttgccttt gacatcctga ctaccgcttt ccattacggc aacagggtat tcgccgaata
     4981 tccagaccac atacaagact attttaagca gtcgtttcct aaaggatatt cgtgggaacg
     5041 cagcttgact ttcgaggacg gaggcatttg tatcgccaga aacgacataa caatggaagg
     5101 ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca atggtccagt
     5161 tatgcagaag aaaacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg
     5221 agtgctgacg ggtgatatta caatggcttt gttgcttgaa ggaaatgccc attaccgatg
     5281 tgacttcaga actacttaca aagctaaaga gaagggtgtc aagttaccag gctaccactt
     5341 tgttgaccac tgtattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta
     5401 tgagcacgct gttgctcatt ctggattgcc tgacaatgcc aggagatctc ccaagaagag
     5461 gaaggtgaga tctgccacca attttagcct cctgaaacaa gcaggcgacg ttgaggagaa
     5521 tccaggaccg tctcccatga tggacgaggc ggaccgccag ctcctgagaa gatgcagggt
     5581 gcgcctagtg agcgagctgc aagtcgcgga gctgtgggac gctctgctga gtcgagagct
     5641 gttcacgcgc gacatgatcg aggatattca gcaggcagga tctggctctc ggcgggatca
     5701 ggctaggcag ctcgtcacag acctggagac tagaggccgc caggctctgc ctctgttcat
     5761 ctcctgctta gaggacacag gccaaggcac cctggcttca ctcttgcaaa gcggtcggca
     5821 agcagccaag caagacccag aggctgttaa acccctagac catctggtgc ctgtggtcct
     5881 gggaccaatg ggactcacag caaaggagca gagagtagtg aagctggacc cgtcacagcc
     5941 tgccgtggga aacctgaccc cagtggtgct cggacctgaa gaactctggc ctgctcgcct
     6001 gaagccagag gttctcagac cagaaacacc tagacccgtg gacattggtt ctggtggagc
     6061 ccatgatgtc tgtgttccag ggaagatcag gggacatgca gatatggcat acaccctgga
     6121 ctctgatccc tgcggccact gtctcatcat caacaatgtg aacttctgcc cttcctcggg
     6181 gctcggcaca cgcacgggct ccaacttgga ccgtgacaaa ctggagcacc gattccgctg
     6241 gctgcgcttc atggtggagg tgaagaacga cctgactgct aagaaaatgg tcacggcttt
     6301 gatggagatg gcacaccgga accaccgtgc cctggactgc tttgtggtgg tcatcctctc
     6361 tcatggctgc caggccagcc acctccagtt cccaggtgct gtttatggga cagatggatg
     6421 ctccgtgtcc attgagaaaa ttgtgaacat ctttaacgga agcggctgcc ctagcctggg
     6481 agggaagccc aaactgttct tcatccaggc ttgcggaggt gagcagaaag accacggctt
     6541 tgaggtggct tgcacttcct ctcagggcag aaccttggac agtgactctg agcctgatgc
     6601 tgtcccctat caggaaggcc caagaccctt ggaccaactg gatgctgtgt caagtttgcc
     6661 tacccctagc gacatccttg tgtcctactc caccttccca ggatttgtgt cttggaggga
     6721 caagaaaagt ggctcctggt acatcgagac tttggacggt attctggaac agtgggctcg
     6781 ctctgaggat ctgcagtccc tccttctcag ggttgccaat gctgtttctg cgaaagggac
     6841 ttacaagcag attcctggct gttttaactt cctgcggaaa aagctgtttt tcaaaacttc
     6901 tgtgcccggg ccagagaacc tgtacttcca ggggctcgag ccctctgccg gagacatgag
     6961 agctgcaaac ctttggccga gcccgctgat gattaaacgc tctaagaaga acagcctggc
     7021 cttgtccctg accgccgacc agatggtcag tgccttgttg gatgccgagc cccccatact
     7081 ctattccgag tatgatccta ccagaccctt cagtgaggct tcgatgatgg gcttactgac
     7141 caacctggca gacagggagc tggttcacat gattaactgg gccaagaggg tgccaggctt
     7201 tgtggatttg accctccacg atcaggtcca cctcctagaa tgtgcctggc tagagatcct
     7261 gatgattggt ctcgtctggc gcagcatgga gcacccagtg aagctactgt ttgctcctaa
     7321 cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga ttttcgacat
     7381 gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg aatttgtgtg
     7441 cctcaaatct attattttgc ttaattctgg agtttacaca tttctgtcca gcaccctgaa
     7501 gtctctggag gaaaaggacc atatccatcg agtgctggac aagatcacag acactttgat
     7561 tcacctgatg gctaaggctg gcctgaccct gcagcagcag catcagagac tcgcacagct
     7621 tctcctgatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtactc
     7681 tatgaagtgc aagaacgtgg tgcccctcta tgatctgctg ctggaggcgg cggacgctca
     7741 ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggaaacag accaaagcca
     7801 cttggctact gcgggctcta cttcatctca ttccttgcaa aagtattaca tcacggggga
     7861 ggcagagggt ttccctgcca cagcaccagg atctacgtag acatgataag atacattgat
     7921 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     7981 gatgctattg ctttatttgt aaccatatta agctgcaata aacaagttaa caacaacaat
     8041 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc ctgcaggact
     8101 ctagtcgcgg ccgctaggga taacagggta atggccgctc ttccgctaat actcaagtac
     8161 aattttaatg gagtactttt ttacttttac tcaagtaaga ttctagccag atacttttac
     8221 ttttaattga gtaaaatttt ccctaagtac ttgtactttc acttgagtaa aatttttgag
     8281 tactttttac acctctgccc tgcatcagca tgtgagcaaa aggccagcaa aaggccagga
     8341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10081 taggggttcc gcgcacattt ccccgaaaag tgccacct
//
