LOCUS       sequence-364019-       10050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modifies pLX_317 backbone to express c-term HA tag (instead of 
            c-term V5 tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Pan J, Kwon JJ, Talamas JA, Borah AA, Vazquez F, Boehm JS, Tsherniak
            A, Zitnik M, McFarland JM, Hahn WC
  TITLE     Sparse dictionary learning recovers pleiotropy from human cell 
            fitness screens.
  JOURNAL   Cell Syst. 2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005.
  PUBMED    35085500
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005."
COMMENT     Sequence Label: pLX_HA
FEATURES             Location/Qualifiers
     source          1..10050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(157..176)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(158..176)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(186..203)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(186..202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(195..217)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      344..799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(431..450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     641..662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        825..929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(930..998,999..1790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1148..1167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1961..2549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2450..2469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2703..2720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2837..2858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2873..2878,2879..2896,2897..2903)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2911..2927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2916..2938
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2935..2951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2935..2951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2970..2990
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2972..2990
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3018..3244
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3245..3425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3472..3597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4090..4323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4508..4552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4783..4993
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4805..5001
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4852..4987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5033..5635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5033..5052)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5529..5549
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5678..5795
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5933..7111
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6164..7102
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7059..7079
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7131..7255
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7280..7285,7286..7303,7304..7310)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7364..8023
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7430..7449)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8365..8670
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8549..8568
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8711..8835)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8843..8869
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8920..9508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8973..8993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9391..9402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9580..9813
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9891..10012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9928..9947)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9982..10001
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
       61 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
      121 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
      181 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      241 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      301 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
      361 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      421 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      481 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      541 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      601 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      661 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      721 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      781 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1021 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1081 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1141 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1201 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1261 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1321 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1381 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1441 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1501 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1561 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1621 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1681 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1741 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1801 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1861 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1921 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1981 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2041 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2101 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2161 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2221 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2281 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2341 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2401 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2461 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2521 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2581 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2641 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2701 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2761 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2821 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2881 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2941 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     3001 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     3061 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     3121 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     3181 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     3241 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3301 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3361 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3421 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     3481 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     3541 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     3601 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     3661 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     3721 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     3781 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     3841 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     3901 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     3961 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     4021 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     4081 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     4141 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     4201 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     4261 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     4321 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     4381 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     4441 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     4501 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     4561 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     4621 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     4681 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     4741 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4801 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4861 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4921 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4981 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     5041 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5101 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5161 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5221 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     5281 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5341 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5401 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5461 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5521 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5581 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
     5641 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     5701 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     5761 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     5821 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
     5881 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     5941 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     6001 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     6061 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     6121 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     6181 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     6241 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     6301 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     6361 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     6421 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     6481 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     6541 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     6601 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     6661 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     6721 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     6781 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     6841 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     6901 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     6961 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     7021 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     7081 aagttttttt cttccatttc aggtgtcgtg aggctagcat cgattgatca acaagtttgt
     7141 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     7201 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     7261 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     7321 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     7381 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     7441 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     7501 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     7561 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     7621 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     7681 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     7741 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     7801 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     7861 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     7921 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     7981 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     8041 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     8101 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     8161 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     8221 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     8281 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     8341 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     8401 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     8461 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     8521 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     8581 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     8641 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     8701 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     8761 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     8821 cttgtacaaa gtggttgata tctacccata cgatgttcca gattacgctt agtaatgaac
     8881 tagtaccggt taagtcgaca atcaacgcgt taagtcgaca atcaacctct ggattacaaa
     8941 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     9001 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     9061 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     9121 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     9181 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     9241 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9301 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     9361 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9421 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9481 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     9541 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     9601 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     9661 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9721 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9781 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     9841 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9901 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9961 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10021 ggctctagct atcccgcccc taactccgcc
//
