LOCUS       sequence-364048-        8144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transposon containing BiFC VC cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, 
            Ashworth A, Lord CJ
  TITLE     Coupling bimolecular PARylation biosensors with genetic screens to 
            identify PARylation targets.
  JOURNAL   Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4.
  PUBMED    29789535
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-22"; volume: "9(1):2016. doi"; pages: " 
            10.1038/s41467-018-04466-4"
COMMENT     Sequence Label: UPATrap-VC-ORF2
FEATURES             Location/Qualifiers
     source          1..8144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2222..2238
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2222..2238
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2272..2283,2284..2471)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    2511..2544
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2664..2697
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(2756..2789)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2966..2999)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3263..3289
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3392..3643
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3580..3601
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3674..3898
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3925..4149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4817..4850
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5025..5058
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(5104..5137)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             5893..6696
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5956..5975)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6566..6585
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(7036..7069)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    join(7293..7455,7456..7467)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7497..7516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7498..7516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7526..7543)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7526..7542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7535..7557)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7684..8111
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7770..7789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7980..8001
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccagctag ctggcggcgg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tgggcccggc tcgatctccg cggccgcatg atcgattgcc gaagttccta
     2521 ttctctagaa agtataggaa cttcggcatc ggcatcaatt catcatcgga tatttgcatc
     2581 gcaatatcaa tccattgccc ggatggccgg atgatcgatg gcggatgcca tgcgatggat
     2641 cggaaatcgc atccattccg gccgaagttc ctattcttca aatagtatag gaacttcggc
     2701 gaattcggaa tcattggcaa tgccatccat cggatttgat gccatgcaat tccggataac
     2761 ttcgtatagc atacattata cgaagttatc cgaaagctgt ccccaatcct cccaccacga
     2821 cccgccgcac tgcagatacc gcaccgtatt ggcaagtagc ccgtaaacgc ggcgaatcgc
     2881 agccagcata gccaggtcca gccgctcgcc ggggcgctgg cgtttggcca ggcggtcgat
     2941 gtgtctgtcc tccggaaggg cccggataac ttcgtatagt acacattata cgaagttatc
     3001 cgggatctgc tgtatctcta agatggctgg cacgtaaaga gggtctcaat aaatatgcct
     3061 tgaattaatg aaaacagtgg tttctgaatc atggcctcca aagagcattt gccttttccc
     3121 ctctcaccct tctgaaggtg cccaggctgc cctggcagga tgcctctttc tctgtggggt
     3181 ggcattctct gctctctctc taatactctt tctttttctt ccctcctctc ccccaactgc
     3241 aggatgcctt tgtggaactg cctacccata cgatgttcca gattacgctc ttatggccat
     3301 ggaggcccga attcggtcga ccgagatctc tcgaggtacc cgtccggcgt gcaaaatccc
     3361 gaacgacctg aaacagaaag tcatgaacca cgacaagcag aagaacggca tcaaggccaa
     3421 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
     3481 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     3541 gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3601 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg ccagcacagt
     3661 ggtcgagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     3721 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3781 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     3841 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     3901 tctgaggcgg aaagctagac tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3961 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4021 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4081 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4141 gctctatggc ttctgaggcg gaaagcttca gcgatggctt ctagctgccc gggtggcatc
     4201 cctgtgaccc ctccccagtg cctcttctgg ccctggaagt tgccactcca gtgcccacca
     4261 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     4321 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     4381 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     4441 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc ttctagcttc agcgatggct
     4501 tctagctgcc cgggtggcat ccctgtgacc cctccccagt gcctcttctg gccctggaag
     4561 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     4621 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     4681 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     4741 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     4801 ctcccgagtc gagcggataa cttcgtataa tgtatgctat acgaagttat ccgcgccagg
     4861 tcgcagatcg tcggtatgga gccgggggtg gtgacgtggg tctggaccat cccggaggta
     4921 agttgcagca gggcgtcccg gcagccggcg ggcgattggt cgtaatccag gataaagacg
     4981 tgcatggaac ggaggcgttt ggccaagacg tccaaggccc acggataact tcgtataatg
     5041 tgtactatac gaagttatcc gatcgatatc catcccaaat atgcgatgga tggatcggat
     5101 gccgaagttc ctatactttc tagagaatag gaacttcggc attgcggccg caaatagatc
     5161 catccaatga agcttgggct gaacatcgag cgccagggct ccgtaaagct actagagcac
     5221 aggcggtgcc ccaacgtcct ggggcctctc cactaataac ggctacttcc aattgattgg
     5281 acgcgccatc ttgcctgcct tatgcatatt cagcggtgaa ctgaatattc atgaacgagg
     5341 cccgtcccgt ccctccctcc ttccccccac ccccggaacc cgctccggag gacccgaagg
     5401 gccccgcctt cattaccgat gcgtaggaca aaccattttc ccgatgtgtg tggggggata
     5461 ctaatgagag actttagctg aaaatgagcc tgaactccga agctgagtaa aaatggccta
     5521 actttatcct ccgttctgta agtcctcggt ttgagtgcac gggaaacccg aaaggaggac
     5581 gacaggacca ggaacattct cctcctcctg tcgcgtcaga aagaacaccc aaccagggag
     5641 ccggagccct agcgtcaaca actccgccgc gcgcgctccg tgtaggccgg tgcgggcggc
     5701 cccgtagcgc aagggagggc gggaaaggaa ggggcgggac acaagggcga atctataaag
     5761 ggcgtcactc agccagttct ctcctcagaa gcgccgagag cgcgaccggg acggttggag
     5821 aagaaggtgg ctcccggaag ggggagagac aaactgccgt aacctctgcc gttcaggatc
     5881 cgtgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     5941 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6001 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6061 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6121 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6181 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6241 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6301 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6361 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6421 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
     6481 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6541 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6601 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6661 atcgccttct atcgccttct tgacgagttc ttctgaccac cggtgacgcc atggccaatc
     6721 gcatcatgga ttccatccga tgcgaatcgc atccatggat gcgccaatcg gattccatgc
     6781 gatgccattt ccgatgcggg atccgaaatt ccagacaagt ttgttgttgg atatgccctc
     6841 gactacaacg agtacttcag gaatttgcag gtaagtaatg cttttttttt tctcgctctc
     6901 atttttcaaa aacccacata aaaattgagg aaagggaaga attgttttct ccttccagca
     6961 cctcgtaatt tgacccgact gatggttccc attagtcaca taaagctgta gtcaagtaca
     7021 gacgtcgaat tcgccgaagt tcctatacta tttgaagaat aggaacttcg gcctcgagca
     7081 tgcatcatat ccatcgcaat cgcatcccca ttcaattcac atcgcatatc cgatgccgat
     7141 atggcatcgg cattgccatc caatcctgat caatatatat atgatgatat ccatatgatg
     7201 atatatatat attaaatgcg ggatatgcca ttccaatgca tccatccgaa tggatccatg
     7261 cggatcatcg catccattgc gataaaggat ctttcttgct tttactttta cttccttaat
     7321 actcaagtac aattttaatg gagtactttt ttacttttac tcaagtaaga ttctagccag
     7381 atacttttac ttttaattga gtaaaatttt ccctaagtac ttgtactttc acttgagtaa
     7441 aatttttgag tactttttac acctctgtca agaaccatat gccggtaccc aattcgccct
     7501 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7561 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7621 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7681 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7741 ccaccacacc cgccgcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7801 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7861 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7921 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7981 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8041 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8101 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtgg
//
