LOCUS       sequence-364060-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing mouse Arkadia proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Xu H, Wu L, Nguyen HH, Mesa KR, Raghavan V, Episkopou V, Littman DR
  TITLE     Arkadia-SKI/SnoN signaling differentially regulates TGF-beta-induced
            iTreg and Th17 cell differentiation.
  JOURNAL   J Exp Med. 2021 Nov 1;218(11). pii: 212614. doi: 
            10.1084/jem.20210777. Epub 2021 Sep 2.
  PUBMED    34473197
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2021-11-1"; volume: "218(11). pii: 212614. doi"; 
            pages: " 10.1084/jem.20210777. Epub 2021 Sep 2"
COMMENT     Sequence Label: MSCV-Arkadia
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    574..915
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             982..1398
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1328..1350
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1366..1382
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             2944..2964
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(4405..4429)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4463..5029
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4479..5031
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4623..4640)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4850..4869
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5079..5567
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     LTR             5623..6137
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6299..6315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6323..6329,6330..6347,6348..6353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6368..6389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6506..6523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6677..7265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6757..6776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7436..8227,8228..8296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8059..8078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8297..8401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8469..8487
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8525..8547)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8647..8666
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8860..8882
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9010..9029
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9229..9235,1..510)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
       61 atacataact gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata
      121 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
      181 tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg
      241 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
      301 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
      361 tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc
      421 tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg
      481 attgactgcc cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct
      541 gcccagggac caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct
      601 gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag
      661 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc
      721 ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga
      781 ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag
      841 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc
      901 gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
      961 ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt
     1021 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
     1081 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
     1141 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
     1201 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
     1261 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
     1321 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
     1381 actccttctc taggcgccgg aattagatcc atgtctcaat ggactcctga atttaacgag
     1441 ctctacaccc taaaagtggc tatgaagagt gggactcctg atgcgcccac tacacaggag
     1501 agtctgaagg cggtccttct gcacccacag cccctagggg ccaccaagag cttccctgca
     1561 gaagtggaga tgattaatag caaagtgggg aatgaattct ctcatctgtg tgatgattct
     1621 caaaaacaag agaaggacat gactggcaac cagcaagaac aagaaaaaag tggtgttgtg
     1681 agaaaaaagc gcaaaagcca gcaggctggc ccctcatatg tgcagaactg tgtcaaagaa
     1741 aatcaggaaa tcctagggcg cagacaacag ctagagacac cgagtgatga ggataatgac
     1801 tcttccttaa gtgagtgtct gtcttctccc tcatctagtc tccattttgg gggctctgat
     1861 acagtgactt cagatgagga taaagaagtc tcggtgagac atacccagcc tgttctgagt
     1921 gctaaaagta gaagtcacag tgcaaggtct cacaagtggc ctcgaactga ggcagatcct
     1981 gtgccaagtt tgttaatgaa aagaccttgt tttcatggca gtgcactaag gagagtcaca
     2041 tgcagaaaga ggctggtgaa gagcagctcc tcgcagagga cacagaagca gaaggagagg
     2101 atgctagtgc agaggaagaa gcgggaggcg ctagctcaga ggaagtatgc tcttctctcc
     2161 agctccagta gctccagtga gaatgacctc agcagcgatt cctcgtccag ctcctccacc
     2221 gatggagaag aggacctgtg tgcatctgcc agtgagaacc ccagcaaccc cgctgctccc
     2281 tcaggaagta ttgatgaaga tgttgtggtg atagaagcct ccttcactcc ccaggtcact
     2341 gccaatgaag aaattaatgt tacctcaact gacagtgaag tggagattgt tacagttgga
     2401 gaaagctatc ggtctcgttc aacccttgga cactccagat ctcattggag ccaaggttca
     2461 agttcgcata caggacggcc acaggagtct cgaaaccgca gtaggatctc tactgttata
     2521 cagcccctga ggcagaatgc agcagaagtt gtggacctta ctgttgatga agatgaacct
     2581 actatagtac caaccacttc tgctagaatg gactcacaaa ctactagtgc ttccattaac
     2641 aattcaaatc catctacctc tgagcaagcc tctgatacta cttcaactgt ggccagtagc
     2701 cagccttcca cagtgtcaga gactgaagct actcttacaa gcaatagtgc aactggttct
     2761 tctgtgggcg atgacgtgag gagaactgca tctagtgctg tcccagaaag tggtcctcct
     2821 gcaatgccaa ggctaccttc ttgctgcccc cagcactcac catgtggagg gacatcacag
     2881 agccatcatg cattagcaca cccacactca agttgttttc agcagcatgg ccaccatttt
     2941 cagcatcacc accatcacca ccatactccc cacccagctg tcccagtttc tccttccttc
     3001 agtgaccctg cttgcccagt ggaaagacct caagtacagg cgccttgtgg agcaaatagc
     3061 agctctggtt ccagctacca tgaccagcag gcattgccag tcgatctgag caacagtgct
     3121 ctcaggactc atggaagtgg gggtttccat ggtgcatctg cttttgaccc ctgctgccct
     3181 gtcacatctt cccgggctgc agtctttggc catcaggctg ctgctgcccc gactcagcca
     3241 ttatcaatag atggctatgg ctccagcatg gttgcccagc cccagcccca gccccctcca
     3301 cagccttctc tttcgtcatg tcggcactac atgccacctc cgtatgcttc cttgacaaga
     3361 ccactgcacc atcaagcctc agcttgccat cactctcatg gaaatgcccc tccccagact
     3421 cagcctcccc ctcaagtgga ttacgtgatt ccgcatcctg tccatgcttt ccattctcag
     3481 atatcttctc atgcagcatc tcaccctgtg gcaccaccgc cgccaacaca tttaggcagt
     3541 acagcggcac ccatccctca gcatcttcct cctgctcacc agccgatttc acaccatatt
     3601 ccagccccag caccttcagc ccagagactg caccctcatg aagtgatgca gaggatggaa
     3661 gttcagagga ggaggatgat gcagcatcca acgcgggcac atgaacggcc cccgccccat
     3721 ccacatagga tgcacccaaa ctatggccat gggcatcata ttcatgtgcc tcaaaccatg
     3781 tcttcacatc ctcgccaggc tccagagaga actgcctggg aactggggat tgaagctgga
     3841 gtaactgcag ctacctacac acctggtgct ctgcaccctc acctggccca ttaccacgcc
     3901 ccgcctcgac ttcatcacct ccagttagga gcacttcctt taatggttcc tgacatggca
     3961 ggctatcctc acatccgtta tatttcttca ggattggatg gagcatcatt cagaggtcct
     4021 tttaggggca attttgagga attgattcat ctggaagaaa gattaggaaa tgtcaatcgt
     4081 ggagcctccc agggaacaat tgaaagatgc acatatccac ataaatataa aaagaggaaa
     4141 ctgcactgca aacaagatgg ggaagaaggg actgaggaag acacagagga aaaatgtact
     4201 atctgtttgt ctattttaga ggaaggtgaa gatgtgaggc gccttccatg tatgcacctt
     4261 ttccaccaag tgtgtgttga tcagtggctg atcaccaata agaagtgccc catatgcaga
     4321 gtggacatcg aggcccagct gccaagtgaa agttgagtcg actggatccg gtaccgaatt
     4381 cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtt cgatgacggt
     4441 atcgataagc ttgatatcga attccgcccc cccccctaac gttactggcc gaagccgctt
     4501 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4561 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4621 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4681 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4741 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4801 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4861 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4921 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4981 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     5041 ccatgggtct tttctgcagt caccgtcctc gaggcaccat gaacccagcc atcagcgtcg
     5101 ctctcctgct ctcagtcttg caggtgtccc gagggcagaa ggtgaccagc ctgacagcct
     5161 gcctggtgaa ccaaaacctt cgcctggact gccgccatga gaataacacc aaggataact
     5221 ccatccagca tgagttcagc ctgacccgag agaagaggaa gcacgtgctc tcaggcaccc
     5281 ttgggatacc cgagcacacg taccgctccc gcgtcaccct ctccaaccag ccctatatca
     5341 aggtccttac cctagccaac ttcaccacca aggatgaggg cgactacttt tgtgagcttc
     5401 gcgtctcggg cgcgaatccc atgagctcca ataaaagtat cagtgtgtat agagacaagc
     5461 tggtcaagtg tggcggcata agcctgctgg ttcagaacac atcctggatg ctgctgctgc
     5521 tgctttccct ctccctcctc caagccctgg acttcatttc tctgtgacgc gtagaattcg
     5581 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     5641 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga
     5701 gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag
     5761 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     5821 gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     5881 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     5941 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
     6001 tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     6061 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     6121 cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta
     6181 ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca
     6241 atactcctga aatagttcat tatggacagc gcagaaagag ctggggagaa ttgtgaaatt
     6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6421 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8401 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     8461 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     8521 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     8581 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     8641 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     8701 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     8761 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     8821 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     8881 cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     8941 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     9001 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     9061 gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc
     9121 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga
     9181 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaa
//
