LOCUS       sequence-364061-        8941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded FRET-based sensor for monitoring cAMP dynamics 
            near a protein of interest. Must pair with GFP11-tagged POI to 
            reconstitute donor fluorescence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke 
            M, Rangamani P, Taylor SS, Mehta S, Zhang J
  TITLE     Phase Separation of a PKA Regulatory Subunit Controls cAMP 
            Compartmentation and Oncogenic Signaling.
  JOURNAL   Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 
            10.1016/j.cell.2020.07.043. Epub 2020 Aug 25.
  PUBMED    32846158
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07.043"; journalName: "Cell"; date: 
            "2020-09-17- 17"; volume: "182"; issue: "6"; pages: "1531-1544.e15"
COMMENT     Sequence Label: pcDNA3.1(+)-FluoSTEP-ICUE
FEATURES             Location/Qualifiers
     source          1..8941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     639..659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..752
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        733..751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             789..1499
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RITVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREVAV
                     AKFAGLGGGMDELYK"
     CDS             3705..4346
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     primer_bind     complement(4402..4419)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4408..4632
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4678..5106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4765..4784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4975..4996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5115..5135)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5120..5449
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5300..5435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5362..5381
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5516..6310
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5570..5589)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6180..6199
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6484..6605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6521..6540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6575..6594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6654..6670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6654..6670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6667..6689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6678..6694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6702..6708,6709..6726,6727..6732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6747..6768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6885..6902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7056..7644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7136..7155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7815..8606,8607..8675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8438..8457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8676..8780)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8855..8874)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      721 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt
      781 aagcttggat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg
      841 tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc
      901 cgtacatggg aactcaaacc atgaggatca cagtcatcga gggaggaccc ctgccatttg
      961 cctttgacat tcttgccacg tcgttcatgt atggcagccg tacttttatc aagtacccga
     1021 aaggcattcc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta
     1081 cgagatacga agatggtgga gtcgtcaccg tcatgcagga caccagcctt gaggatggct
     1141 gtctcgttta ccacgtccaa gtcagagggg taaactttcc ctccaatggt cccgtgatgc
     1201 agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc
     1261 tgaggggata cactcatatg gcactgaaag ttgatggtgg tggccatctg tcttgctctt
     1321 tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtatccatg
     1381 ccgttgatca ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc gtagtacaac
     1441 gcgaagtcgc agttgccaag ttcgccgggc ttggtggtgg gatggacgag ctgtacaagg
     1501 ctggtacccc cgtgggaact catgagatgg aggaggagtt ggccgaagct gtggccctgc
     1561 tctcccagcg ggggcctgac gccctgctca ctgtggcact tcgaaagccc ccaggtcagc
     1621 gcacggatga agagctggac ctcatctttg aggagctgct gcacatcaag gctgtggccc
     1681 acctctccaa ctcggtgaag cgagaattag cggctgttct gctctttgaa ccacacagca
     1741 aggcagggac cgtgttgttc agccaggggg acaagggcac ttcgtggtac attatctgga
     1801 agggatctgt caacgtggtg acccatggca aggggctggt gaccaccctg catgagggag
     1861 atgattttgg acagctggct ctggtgaatg atgcaccccg ggcagccacc atcatcctgc
     1921 gagaagacaa ctgtcatttc ctgcgtgtgg acaagcagga cttcaaccgt atcatcaagg
     1981 atgtggaggc aaagaccatg cggctggaag aacatggcaa agtggtgctg gtgctggaga
     2041 gagcctctca gggcgccggc ccttcccgac ccccaacccc aggcaggaac cggtatacag
     2101 tgatgtctgg caccccagag aagatcctag agcttctgtt ggaggccatg ggaccagatt
     2161 ccagtgctca tgaccaaaca gagacattcc tcagcgactt cctcctgacc cacagggtct
     2221 tcatgcccag cgcccaactc tgcgctgccc ttctgcacca cttccatgtg gagcctgcgg
     2281 gtggcagcga gcaggagcgc agcacctacg tctgcaacaa gaggcagcag atcttgcggc
     2341 tggtcagcca gtgggtggcc ctgtatggct ccatgctcca cactgaccct gtggccacca
     2401 gcttcctcca gaaactctca gacctggtgg gcagggacac ccgactcagc aacctgctga
     2461 gggagcagtg gccagagagg cggcgatgcc acaggttgga gaatggctgt gggaatgcat
     2521 ctcctcagat gaaggcccgg aacttgcctg tttggctccc caaccaggac gagccccttc
     2581 ctggcagcag ctgtgccatc caagttgggg ataaagtccc ctatgacatc tgccggccag
     2641 accactcagt gttgaccctg cagctgcctg tgacagcctc cgtgagagag gtgatggcag
     2701 cgttggccca ggaggatggc tggaccaagg ggcaggtgct ggtgaaggtc aattctgcag
     2761 gtgatgccat tggcctgcag ccagatgccc gtggtgtggc cacatctctg gggctcaatg
     2821 agcgtctctt tgttgtcaac ccacaggaag cgcatgagct gatcccacac cctgaccagc
     2881 tggggcccac tgtgggctct gctgaggggc tggacctggt gagtgccaag gacctggcag
     2941 gccagctgac ggaccacgac tggagcctct tcaacagtat ccaccaggtg gagctgatcc
     3001 actatgtgct gggcccccag catctgcggg atgtcaccac cgccaacctg gagcgcttca
     3061 tgcgccgctt caatgagctg cagtactggg tggccaccga gctgtgtctc tgccccgtgc
     3121 ccggcccccg ggcccagctg ctcaggaagt tcattaagct ggcggcccac ctcaaggagc
     3181 agaagaatct caattccttc tttgccgtca tgtttggcct cagcaactcg gccatcagcc
     3241 acctagccca cacctgggag cggctgcccc acaaagtccg gaagctgtac tccgccctcg
     3301 agaggctgct ggacccctca tggaaccacc gggtataccg actggccctc gccaagctct
     3361 cccctcctgt catccccttc atgccccttc ttctcaaaga catgaccttc attcatgagg
     3421 gaaaccacac actagtggag aatctcatca actttgagaa gatgagaatg atggccagag
     3481 tcgcgcggat gctgcaccac tgccgaagcc acaaccctgt gcctctctca ccactcagaa
     3541 gccgagtttc ccacctccac gaggacagcc aggtggcgag gatttccaca tgctcggagc
     3601 agtccctgag cacccggagt ccagccagca cctgggctta tgtccagcag ctgaaggtca
     3661 ttgacaacca gcgggaactc tcccgcctct cccgagagct ggagatgtcc aaaggagaag
     3721 aactgtttac cggtgttgtg ccaattttgg ttgaactcga tggtgatgtc aacggacata
     3781 agttctcagt gagaggcgaa ggagaaggtg acgccaccat tggaaaattg actcttaaat
     3841 tcatctgtac tactggtaaa cttcctgtac catggccgac tctcgtaaca acgcttacgt
     3901 acggagttca gtgcttttcg agatacccag accatatgaa aagacatgac ttttttaagt
     3961 cggctatgcc tgaaggttac gtgcaagaaa gaacaatttc gttcaaagat gatggaaaat
     4021 ataaaactag agcagttgtt aaatttgaag gagatacttt ggttaaccgc attgaactga
     4081 aaggaacaga ttttaaagaa gatggtaata ttcttggaca caaactcgaa tacaatttta
     4141 atagtcataa cgtatacatc actgctgata agcaaaagaa cggaattaaa gcgaatttca
     4201 cagtacgcca taatgtagaa gatggcagtg ttcaacttgc cgaccattac caacaaaaca
     4261 cccctattgg agacggtccg gtacttcttc ctgataatca ctacctctca acacaaacag
     4321 tcctgagcaa agatccaaat gaaaaaggaa caggtggcgg cggaagttag tctagagggc
     4381 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4441 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4501 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4561 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4621 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4681 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4741 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4801 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4861 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4921 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4981 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5041 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5101 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5221 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5281 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5341 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     5401 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5461 agcttatata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     5521 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5581 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5641 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     5701 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5761 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5821 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5881 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5941 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6001 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6061 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6121 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6181 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6241 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6301 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6361 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6421 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6481 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6541 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6601 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6661 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6721 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6781 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6841 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6901 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6961 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7621 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7741 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7801 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7861 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7921 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7981 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8041 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8101 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8161 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8221 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8281 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8341 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8401 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8461 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8521 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8581 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8641 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8701 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8761 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     8821 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8881 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8941 t
//
