LOCUS       sequence-364063-        3768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35E2B_STOP
FEATURES             Location/Qualifiers
     source          1..3768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1325..1424)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1441..1460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1442..1460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1465..1481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1465..1481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1583..1602)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1594..2403
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1661..1680)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2550..3138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3039..3058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3292..3309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3468..3495)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3587..3673)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3722..3744
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3736..3753
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3737..3753
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag ctcctctgtg
      121 aagacacctg ccctggagga gctggtgcca ggaagcgagg agaagccaaa gggcaggtct
      181 ccactgagct ggggatccct gttcggacac cgctctgaga agatcgtgtt tgccaagagc
      241 gacggcggca ccgatgagaa cgtgctgacc gtgacaatca ccgagaccac agtgatcgag
      301 tccgacctgg gcgtgtggag ctccagggcc ctgctgtacc tgacactgtg gttctttttc
      361 tctttttgca ccctgttcct gaataagtat atcctgtccc tgctgggagg agagccttct
      421 atgctgggag ccgtgcagat gctgagcacc acagtgatcg gctgcgtgaa gaccctggtg
      481 ccatgctgtc tgtaccagca caaggccagg ctgtcctatc cccctaactt tctgatgaca
      541 atgctgttcg tgggcctgat gcgctttgca accgtggtgc tgggactggt gagcctgaag
      601 aatgtggccg tgtccttcgc cgagacagtg aagtctagcg cccccatctt taccgtgatc
      661 atgtctcgga tgatcctggg cgagtacaca ggcctgctgg tgaacctgag cctgatccct
      721 gtgatgggag gactggccct gtgcacagca accgagatca gctttaatgt gctgggcttc
      781 tccgccgccc tgtctaccaa catcatggac tgtctgcaga acgtgttcag caagaagctg
      841 ctgagcggcg ataagtacag attctccgcc ccagagctgc agttttatac atctgccgca
      901 gcagtggcaa tgctggtgcc agcacgggtg tttttcaccg acgtgcctgt gatcggcaga
      961 tccggcaagt ccttctctta caaccaggac gtggtgctgc tgctgctgac agatggcgtg
     1021 ctgtttcacc tgcagagcgt gaccgcctat gccctgatgg gcaagatctc ccccgtgaca
     1081 ttctctgtgg ccagcaccgt gaagcacgcc ctgagcatct ggctgtccgt gatcgtgttt
     1141 ggcaacaaga tcaccagcct gtccgccgtg ggaacagccc tggtgaccgt gggcgtgctg
     1201 ctgtataata aggcaaggca gcaccagcag gaggccctgc agtccctggc agcagcaaca
     1261 ggaagggcac cagacgatac cgtggagcca ctgctgcccc aggatcctag acagcacccc
     1321 taagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1381 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1441 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1501 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1561 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1621 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1681 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1741 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1801 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1861 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1921 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1981 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2041 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2101 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2161 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2221 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2281 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2341 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2401 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2461 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2521 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2581 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2641 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2701 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2761 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2821 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2881 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2941 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3001 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3061 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3121 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3181 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3241 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3301 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3361 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3421 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3481 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3541 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3601 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3661 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3721 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
