LOCUS       sequence-364064-        3351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35E2A_STOP
FEATURES             Location/Qualifiers
     source          1..3351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(908..1007)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1024..1043)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1025..1043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1048..1064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1048..1064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1166..1185)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1177..1986
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1244..1263)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2133..2721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2622..2641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2875..2892
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3051..3078)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3170..3256)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3305..3327
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3319..3336
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3320..3336
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag ctcctctgtg
      121 aagacaccag ccctggagga gctggtgcca ggaagcgagg agaagccaaa gggccggtcc
      181 cctctgtctt ggggaagcct gttcggccac agatccgaga agatcgtgtt tgccaagtct
      241 gacggcggca ccgatgagaa cgtgctgacc gtgacaatca ccgagaccac agtgatcgag
      301 tccgacctgg gcgtgtggag ctcccgggcc ctgctgtacc tgacactgtg gttctttttc
      361 tctttttgca ccctgttcct gaataagtat atcctgagcc tgctgggagg agagccatcc
      421 atgctgggag ccgtgcagat gctgagcacc acagtgatcg gctgcgtgaa gacactggtg
      481 ccctgctgtc tgtaccagca caaggccagg ctgtcctatc cccctaactt tctgatgaca
      541 atgctgttcg tgggcctgat gcgctttgcc accgtggtgc tgggcctggt gtctctgaag
      601 aatgtggccg tgagcttcgc cgagacagtg aagtctagcg cccccatctt taccgtgatc
      661 atgtctagga tgatcctggg agagtacacc ggccgcccta gcgacaggga ggagagggag
      721 gagctgcagc tgcagcctgg aaggggagca gcagcctccg atcggagatc tccagtgcca
      781 ccatccgaga ggcacggcgt gagaccacac ggagagaacc tgcccggcga tttccaggtg
      841 cctcaggccc tgcacagagt ggccctgagc atggccctgc cttgcccaat gctgcctgcc
      901 tcctaagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      961 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1021 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1081 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1141 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1201 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1261 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1321 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1381 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1441 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1501 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1561 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1621 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1681 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     1741 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1801 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1861 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     1921 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     1981 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2041 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2101 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2161 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2221 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2281 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2341 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2401 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2461 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2521 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2581 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2641 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2701 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2761 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2821 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2881 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2941 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3001 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3061 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3121 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3181 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3241 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3301 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
