LOCUS       sequence-364065-       10109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke 
            M, Rangamani P, Taylor SS, Mehta S, Zhang J
  TITLE     Phase Separation of a PKA Regulatory Subunit Controls cAMP 
            Compartmentation and Oncogenic Signaling.
  JOURNAL   Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 
            10.1016/j.cell.2020.07.043. Epub 2020 Aug 25.
  PUBMED    32846158
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07.043"; journalName: "Cell"; date: 
            "2020-09-17- 17"; volume: "182"; issue: "6"; pages: "1531-1544.e15"
COMMENT     Sequence Label: pcDNA3-PDE4D2cat-ICUE4
FEATURES             Location/Qualifiers
     source          1..10109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1933..1935,1936..1938,1939..2649)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1933..1935,1936..1938,1939..2649)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1933..1935,1936..1938,1939..2649)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(5014..5016,5017..5019,5020..5532)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     promoter        complement(5662..5680)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5663..5680)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5700..5717)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5706..5930
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5976..6404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6063..6082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6273..6294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6413..6433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6418..6747
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6598..6733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6660..6679
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6814..7608
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6868..6887)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7478..7497
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7782..7903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7819..7838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7873..7892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7952..7968)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7952..7968)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7965..7987)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7976..7992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8000..8006,8007..8024,8025..8030))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8045..8066
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8183..8200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8354..8942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8434..8453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9113..9904,9905..9973))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9736..9755
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9974..10078)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccgc caccatgact
      901 gaacaagaag atgtccttgc caaggaacta gaagatgtga acaaatgggg tcttcatgtt
      961 ttcagaatag cagagttgtc tggtaaccgg cccttgactg ttatcatgca caccattttt
     1021 caggaacggg atttattaaa aacatttaaa attccagtag atactttaat tacatatctt
     1081 atgactctcg aagaccatta ccatgctgat gtggcctatc acaacaatat ccatgctgca
     1141 gatgttgtcc agtctactca tgtgctatta tctacacctg ctttggaggc tgtgtttaca
     1201 gatttggaga ttcttgcagc aatttttgcc agtgcaatac atgatgtaga tcatcctggt
     1261 gtgtccaatc aatttctgat caatacaaac tctgaacttg ccttgatgta caatgattcc
     1321 tcagtcttag agaaccatca tttggctgtg ggctttaaat tgcttcagga agaaaactgt
     1381 gacattttcc agaatttgac caaaaaacaa agacaatctt taaggaaaat ggtcattgac
     1441 atcgtacttg caacagatat gtcaaaacac atgaatctac tggctgattt gaagactatg
     1501 gttgaaacta agaaagtgac aagctctgga gttcttcttc ttgataatta ttccgatagg
     1561 attcaggttc ttcagaatat ggtgcactgt gcagatctga gcaacccaac aaagcctctc
     1621 cagctgtacc gccagtggac ggaccggata atggaggagt tcttccgcca aggagaccga
     1681 gagagggaac gtggcatgga gataagcccc atgtgtgaca agcacaatgc ttccgtggaa
     1741 aaatcacagg tgggcttcat agactatatt gttcatcccc tctgggagac atgggcagac
     1801 ctcgtccacc ctgacgccca ggatattttg gacactttgg aggacaatcg tgaatggtac
     1861 cagagcacaa tccctcaggg tggaacagga ggttcaggag gtaccggtgg atctggcggt
     1921 actggtggtt ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1981 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
     2041 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2101 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
     2161 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2221 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2281 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2341 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     2401 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
     2461 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2521 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     2581 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag
     2641 ctgtacaagg ctggtacccc cgtgggaact catgagatgg aggaggagtt ggccgaagct
     2701 gtggccctgc tctcccagcg ggggcctgac gccctgctca ctgtggcact tcgaaagccc
     2761 ccaggtcagc gcacggatga agagctggac ctcatctttg aggagctgct gcacatcaag
     2821 gctgtggccc acctctccaa ctcggtgaag cgagaattag cggctgttct gctctttgaa
     2881 ccacacagca aggcagggac cgtgttgttc agccaggggg acaagggcac ttcgtggtac
     2941 attatctgga agggatctgt caacgtggtg acccatggca aggggctggt gaccaccctg
     3001 catgagggag atgattttgg agaactggct ctggtgaatg atgcaccccg ggcagccacc
     3061 atcatcctgc gagaagacaa ctgtcatttc ctgcgtgtgg acaagcagga cttcaaccgt
     3121 atcatcaagg atgtggaggc aaagaccatg cggctggaag aacatggcaa agtggtgctg
     3181 gtgctggaga gagcctctca gggcgccggc ccttcccgac ccccaacccc aggcaggaac
     3241 cggtatacag tgatgtctgg caccccagag aagatcctag agcttctgtt ggaggccatg
     3301 ggaccagatt ccagtgctca tgaccaaaca gagacattcc tcagcgactt cctcctgacc
     3361 cacagggtct tcatgcccag cgcccaactc tgcgctgccc ttctgcacca cttccatgtg
     3421 gagcctgcgg gtggcagcga gcaggagcgc agcacctacg tctgcaacaa gaggcagcag
     3481 atcttgcggc tggtcagcca gtgggtggcc ctgtatggct ccatgctcca cactgaccct
     3541 gtggccacca gcttcctcca gaaactctca gacctggtgg gcagggacac ccgactcagc
     3601 aacctgctga gggagcagtg gccagagagg cggcgatgcc acaggttgga gaatggctgt
     3661 gggaatgcat ctcctcagat gaaggcccgg aacttgcctg tttggctccc caaccaggac
     3721 gagccccttc ctggcagcag ctgtgccatc caagttgggg ataaagtccc ctatgacatc
     3781 tgccggccag accactcagt gttgaccctg cagctgcctg tgacagcctc cgtgagagag
     3841 gtgatggcag cgttggccca ggaggatggc tggaccaagg ggcaggtgct ggtgaaggtc
     3901 aattctgcag gtgatgccat tggcctgcag ccagatgccc gtggtgtggc cacatctctg
     3961 gggctcaatg agcgtctctt tgttgtcaac ccacaggaag cgcatgagct gatcccacac
     4021 cctgaccagc tggggcccac tgtgggctct gctgaggggc tggacctggt gagtgccaag
     4081 gacctggcag gccagctgac ggaccacgac tggagcctct tcaacagtat ccaccaggtg
     4141 gagctgatcc actatgtgct gggcccccag catctgcggg atgtcaccac cgccaacctg
     4201 gagcgcttca tgcgccgctt caatgagctg cagtactggg tggccaccga gctgtgtctc
     4261 tgccccgtgc ccggcccccg ggcccagctg ctcaggaagt tcattaagct ggcggcccac
     4321 ctcaaggagc agaagaatct caattccttc tttgccgtca tgtttggcct cagcaactcg
     4381 gccatcagcc acctagccca cacctgggag cggctgcccc acaaagtccg gaagctgtac
     4441 tccgccctcg agaggctgct ggacccctca tggaaccacc gggtataccg actggccctc
     4501 gccaagctct cccctcctgt catccccttc atgccccttc ttctcaaaga catgaccttc
     4561 attcatgagg gaaaccacac actagtggag aatctcatca actttgagaa gatgagaatg
     4621 atggccagag tcgcgcggat gctgcaccac tgccgaagcc acaaccctgt gcctctctca
     4681 ccactcagaa gccgagtttc ccacctccac gaggacagcc aggtggcgag gatttccaca
     4741 tgctcggagc agtccctgag cacccggagt ccagccagca cctgggctta tgtccagcag
     4801 ctgaaggtca ttgacaacca gcgggaactc tcccgcctct cccgagagct ggagccagag
     4861 ctcatgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag
     4921 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4981 gacgagctgt acaagggagg taccggtgga tctatggtga gcaagggcga ggagctgttc
     5041 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5101 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     5161 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctt
     5221 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5281 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5341 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5401 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5461 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     5521 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     5581 ggcgacggcc ccgtgctgta agaattctgc agatatccat cacactggcg gccgctcgag
     5641 catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
     5701 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5761 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5821 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5881 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5941 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6001 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6061 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6121 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6181 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6241 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6301 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6361 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     6421 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6481 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6541 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6601 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6661 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6721 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6781 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6841 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6901 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6961 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7021 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7081 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7141 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7201 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7261 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7321 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     7381 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7441 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7501 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7561 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     7621 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7681 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7741 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     7801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7921 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7981 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8041 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8101 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8161 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8761 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9121 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9181 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9241 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9301 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9361 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9421 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9481 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9541 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9601 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9661 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9721 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9781 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9841 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9901 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9961 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10021 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10081 catttccccg aaaagtgcca cctgacgtc
//
