LOCUS       sequence-364095-        8147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Yim WW, Yamamoto H, Mizushima N
  TITLE     Annexins A1 and A2 are recruited to larger lysosomal injuries 
            independently of ESCRTs to promote repair.
  JOURNAL   FEBS Lett. 2022 Mar 11. doi: 10.1002/1873-3468.14329.
  PUBMED    35274304
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2022 Mar 11. doi: 10.1002/1873-3468.14329."
COMMENT     Sequence Label: pMRX-IZ-ANXA11-mRuby3
FEATURES             Location/Qualifiers
     source          1..8147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    742..1099
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1164..1580
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1510..1532
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1548..1564
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1590..1964
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1902..1918
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    4286..4852
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4302..4854
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4446..4463)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4673..4692
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4872..5246
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5476..5945
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6075..6092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6246..6834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6326..6345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6998..7789,7790..7858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7621..7640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7859..7963)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8031..8049
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgctagaga accatcagat gtttccaggg tgccccaagg
      421 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
      481 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
      541 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
      601 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
      661 tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc
      721 accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt ccgattgtct
      781 agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact agctctgtat
      841 ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg
      901 tcccagggac ttcgggggcc gtttttgtgg cccgacctga gtccaaaaat cccgatcgtt
      961 ttggactctt tggtgcaccc cccttagagg agggatatgt ggttctggta ggagacgaga
     1021 acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttgggac cgaagccgcg
     1081 ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
     1141 atttgtctga aaatatgggc ccgggccaga ctgttaccac tcccttaagt ttgaccttag
     1201 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1261 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1321 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1381 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1441 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1501 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1561 tcactccttc tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc
     1621 cccttgtaaa cttccctgac cctgacatga caagagttac taacagcccc tctctccaag
     1681 ctcacttaca ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct
     1741 accaagaaca actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt
     1801 gggtccgccg acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc
     1861 tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc
     1921 acgtgaaggc tgccgacccc gggggtggac catcctctag actgccggat ctgccatcga
     1981 tataaagctt aaaggatcca tgagctaccc tggctatccc ccgcccccag gtggctaccc
     2041 accagctgca ccaggtggtg gtccctgggg aggtgctgcc taccctcctc cgcccagcat
     2101 gccccccatc gggctggata acgtggccac ctatgcgggg cagttcaacc aggactatct
     2161 ctcgggaatg gcggccaaca tgtctgggac atttggagga gccaacatgc ccaacctgta
     2221 ccctggggcc cctggggctg gctacccacc agtgccccct ggcggctttg ggcagccccc
     2281 ctctgcccag cagcctgttc ctccctatgg gatgtatcca cccccaggag gaaacccacc
     2341 ctccaggatg ccctcatatc cgccataccc aggggcccct gtgccgggcc agcccatgcc
     2401 accccccgga cagcagcccc caggggccta ccctgggcag ccaccagtga cctaccctgg
     2461 tcagcctcca gtgccactcc ctgggcagca gcagccagtg ccgagctacc caggataccc
     2521 ggggtctggg actgtcaccc ccgctgtgcc cccaacccag tttggaagcc gaggcaccat
     2581 cactgatgct cccggctttg accccctgcg agatgccgag gtcctgcgga aggccatgaa
     2641 aggcttcggg acggatgagc aggccatcat tgactgcctg gggagttgct ccaacaagca
     2701 gcggcagcag atcctacttt ccttcaagac ggcttacggc aaggatttga tcaaagatct
     2761 gaaatctgaa ctgtcaggaa actttgagaa gacaatcttg gctctgatga agaccccagt
     2821 cctctttgac atttatgaga taaaggaagc catcaagggg gttggcactg atgaagcctg
     2881 cctgattgag atcctcgctt cccgcagcaa tgagcacatc cgagaattaa acagagccta
     2941 caaagcagaa ttcaaaaaga ccctggaaga ggccattcga agcgacacat cagggcactt
     3001 ccagcggctc ctcatctctc tctctcaggg aaaccgtgat gaaagcacaa acgtggacat
     3061 gtcactcgcc cagagagatg cccaggagct gtatgcggcc ggggagaacc gcctgggaac
     3121 agacgagtcc aagttcaatg cggttctgtg ctcccggagc cgggcccacc tggtagcagt
     3181 tttcaatgag taccagagaa tgacaggccg ggacattgag aagagcatct gccgggagat
     3241 gtccggggac ctggaggagg gcatgctggc cgtggtgaaa tgtctcaaga ataccccagc
     3301 cttctttgcg gagaggctca acaaggccat gaggggggca ggaacaaagg accggaccct
     3361 gattcgcatc atggtgtctc gcagcgagac cgacctcctg gacatcagat cagagtataa
     3421 gcggatgtac ggcaagtcgc tgtaccacga catctcggga gatacttcag gggattaccg
     3481 gaagattctg ctgaagatct gtggtggcaa tgacggatcc gggaattcgg gctctggttc
     3541 aggcgtgagc aagggcgagg agctcatcaa ggagaacatg cggatgaaag tcgtcatgga
     3601 aggctctgtc aatgggcacc agttcaagtg taccggagaa ggggaagggc gtccctatga
     3661 aggcgtacaa accatgcgca tcaaagtgat agagggcggg ccactccctt ttgccttcga
     3721 cattctggct acctccttca tgtatggcag tcggacattc atcaagtatc ccgcagacat
     3781 tccggacttc ttcaaacagt cctttccaga aggctttaca tgggagaggg tgactaggta
     3841 cgaagatggc ggagtggtta ctgtgactca ggacacgtca ttggaggacg gagagctggt
     3901 gtacaatgtg aaagtacgcg gagtgaactt tcccagcaat ggtcctgtta tgcagaagaa
     3961 aaccaagggt tgggaaccca atacggagat gatgtatcca gctgatggtg gcctgagggg
     4021 atacacagac atagccctga aagtcgatgg aggtggccat ctgcactgca actttgtgac
     4081 cacctacaga agcaagaaaa ctgtggggaa cattaagatg cctggagtgc atgcagtcga
     4141 tcacagactt gagagaatcg aggagtctga taacgagaca tacgtggtac aacgagaagt
     4201 tgccgttgcg aagtacagca atctgggcgg gggcatggac gagctgtaca agtaatgatt
     4261 tctcgagagc ggccgctacg taaattccgc ccccccccct aacgttactg gccgaagccg
     4321 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     4381 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     4441 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     4501 ggaagtttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     4561 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     4621 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     4681 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     4741 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     4801 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     4861 caaccgtcga catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     4921 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     4981 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     5041 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     5101 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     5161 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     5221 acttcgtggc cgaggagcag gactgagtcg accgcccgcc ccacgacccg cagcgcccga
     5281 ccgaaaggag cgcacgaccc catggctcga cgataaaata aaagatttta tttagtctcc
     5341 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     5401 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     5461 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     5521 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     5581 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     5641 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     5701 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     5761 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     5821 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     5881 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     5941 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     6001 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6061 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat
     6961 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7021 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7081 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7141 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7201 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7261 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7321 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7381 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7441 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7501 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7561 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     7621 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7681 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7741 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7801 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7861 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7921 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7981 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     8041 tcacgaggcc ctttcgtctt cactatagga gcttggccca ttgcatacgt tgtatccata
     8101 tcataatatg tacatttata ttggctcatg tccaacatta ccgccat
//
