LOCUS       sequence-364096-        8030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Yim WW, Yamamoto H, Mizushima N
  TITLE     Annexins A1 and A2 are recruited to larger lysosomal injuries 
            independently of ESCRTs to promote repair.
  JOURNAL   FEBS Lett. 2022 Mar 11. doi: 10.1002/1873-3468.14329.
  PUBMED    35274304
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2022 Mar 11. doi: 10.1002/1873-3468.14329."
COMMENT     Sequence Label: pMRX-IZ-ANXA7-mRuby3
FEATURES             Location/Qualifiers
     source          1..8030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    752..1109
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1174..1590
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1520..1542
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1558..1574
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1600..1974
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1912..1928
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    4179..4745
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4195..4747
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4339..4356)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4566..4585
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4765..5139
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5369..5838
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5968..5985)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6139..6727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6219..6238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6891..7682,7683..7751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7514..7533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7752..7856)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7924..7942
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atgctagaga accatcagat gtttccaggg
      421 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      481 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      601 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
      781 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc
      901 ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga gtccaaaaat
      961 cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt ggttctggta
     1021 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttgggac
     1081 cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact
     1141 gtgtttctgt atttgtctga aaatatgggc ccgggccaga ctgttaccac tcccttaagt
     1201 ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc
     1261 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1321 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1381 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1441 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct
     1501 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt
     1561 tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat cttatatggg
     1621 gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac taacagcccc
     1681 tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg gagacctctg
     1741 gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta ccgagtcggc
     1801 gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg gaaaggacct
     1861 tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc agcttggata
     1921 cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag actgccggat
     1981 ctgccatcga tataaagctt aaaggatcca tgtcataccc aggctatccc ccaacaggct
     2041 acccaccttt ccctggatat cctcctgcag gtcaggagtc atcttttccc ccttctggtc
     2101 agtatcctta tcctagtggc tttcctccaa tgggaggagg tgcctaccca caagtgccaa
     2161 gtagtggcta cccaggagct ggaggctacc ctgcgcctgg aggttatcca gcccctggag
     2221 gctatcctgg tgccccacag ccagggggag ctccatccta tcccggagtt cctccaggcc
     2281 aaggatttgg agtcccacca ggtggagcag gcttttctgg gtatccacag ccaccttcac
     2341 agtcttatgg aggtggtcca gcacaggttc cactacctgg tggctttcct ggaggacaga
     2401 tgccttctca gtatcctgga ggacaaccta cttaccctag tcagcctgcc acagtgactc
     2461 aggtcactca aggaactatc cgaccagctg ccaacttcga tgctataaga gatgcagaaa
     2521 ttcttcgtaa ggcaatgaag ggttttggga cagatgagca ggcaattgtg gatgtggtgg
     2581 ccaaccgttc caatgatcag aggcaaaaaa ttaaagcagc atttaagacc tcctatggca
     2641 aggatttaat caaagatctc aaatcagagt taagtggaaa tatggaagaa ctgatcctgg
     2701 ccctcttcat gcctcctacg tattacgatg cctggagctt acggaaagca atgcagggag
     2761 caggaactca ggaacgtgta ttgattgaga ttttgtgcac aagaacaaat caggaaatcc
     2821 gagaaattgt cagatgttat cagtcagaat ttggacgaga ccttgaaaag gacattaggt
     2881 cagatacatc aggacatttt gaacgtttac ttgtgtccat gtgccaggga aatcgtgatg
     2941 agaaccagag tataaaccac caaatggctc aggaagatgc tcagcgtctc tatcaagctg
     3001 gtgaggggag actagggacc gatgaatctt gctttaacat gatccttgcc acaagaagct
     3061 ttcctcagct gagagctacc atggaggctt attctaggat ggctaatcga gacttgttaa
     3121 gcagtgtgag ccgtgagttt tccggatatg tagaaagtgg tttgaagacc atcttgcagt
     3181 gtgccctgaa ccgccctgcc ttctttgctg agaggctcta ctatgctatg aaaggtgctg
     3241 gcacagatga ctccaccctg gtccggattg tggtcactcg aagtgagatt gaccttgtac
     3301 aaataaaaca gatgttcgct cagatgtatc agaagactct gggcacaatg attgcaggtg
     3361 acacgagtgg agattaccga agacttcttc tggctattgt gggccaggga tccgggaatt
     3421 cgggctctgg ttcaggcgtg agcaagggcg aggagctcat caaggagaac atgcggatga
     3481 aagtcgtcat ggaaggctct gtcaatgggc accagttcaa gtgtaccgga gaaggggaag
     3541 ggcgtcccta tgaaggcgta caaaccatgc gcatcaaagt gatagagggc gggccactcc
     3601 cttttgcctt cgacattctg gctacctcct tcatgtatgg cagtcggaca ttcatcaagt
     3661 atcccgcaga cattccggac ttcttcaaac agtcctttcc agaaggcttt acatgggaga
     3721 gggtgactag gtacgaagat ggcggagtgg ttactgtgac tcaggacacg tcattggagg
     3781 acggagagct ggtgtacaat gtgaaagtac gcggagtgaa ctttcccagc aatggtcctg
     3841 ttatgcagaa gaaaaccaag ggttgggaac ccaatacgga gatgatgtat ccagctgatg
     3901 gtggcctgag gggatacaca gacatagccc tgaaagtcga tggaggtggc catctgcact
     3961 gcaactttgt gaccacctac agaagcaaga aaactgtggg gaacattaag atgcctggag
     4021 tgcatgcagt cgatcacaga cttgagagaa tcgaggagtc tgataacgag acatacgtgg
     4081 tacaacgaga agttgccgtt gcgaagtaca gcaatctggg cgggggcatg gacgagctgt
     4141 acaagtaatg atttctcgag agcggccgct acgtaaattc cgcccccccc cctaacgtta
     4201 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4261 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     4321 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     4381 aagcagttcc tctggaagtt tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     4441 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4501 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4561 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4621 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4681 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     4741 gataatatgg ccacaaccgt cgacatggcc aagttgacca gtgccgttcc ggtgctcacc
     4801 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     4861 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     4921 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     4981 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     5041 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     5101 ggcaactgcg tgcacttcgt ggccgaggag caggactgag tcgaccgccc gccccacgac
     5161 ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct cgacgataaa ataaaagatt
     5221 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     5281 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     5341 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     5401 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     5461 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     5521 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     5581 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     5641 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     5701 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     5761 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     5821 gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag
     5881 tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5941 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6001 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6061 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6121 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6541 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6661 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6721 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6781 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg
     6841 gccggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7501 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7681 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7741 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7861 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     7921 aaaaataggc gtatcacgag gccctttcgt cttcactata ggagcttggc ccattgcata
     7981 cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca
//
