LOCUS       sequence-364129-        8884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic PHR domain coupled to GFP and STAT2 activation domain; 
            binds to CIBN upon blue light exposure.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
  TITLE     Transcription activation is enhanced by multivalent interactions 
            independent of phase separation.
  JOURNAL   Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017.
  PUBMED    35537448
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017."
COMMENT     Sequence Label: PHR-GFP-STAT2
FEATURES             Location/Qualifiers
     source          1..8884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..165
                     /gene="bla"
                     /label=AmpR promoter
     promoter        167..524
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        167..358
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(190..210)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      375..510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             559..1353
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             559..1353
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(613..632)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1223..1242
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1541..1560)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1585..1632
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1961..2549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2450..2469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2724..3027
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3028..3231
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3182..3202
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3288..4781
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(4791..4793,4794..4796,4797..5507)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4791..4793,4794..4796,4797..5507)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4836..4857)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5097..5116)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5444..5465
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5541..5561
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8199..8208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8335..8456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8372..8391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8426..8445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(join(8463..8884,1..34))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8600..8621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8812..8831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
       61 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      121 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
      181 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      241 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      481 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
      541 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
      601 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
      661 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
      721 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
      781 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
      841 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
      901 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
      961 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     1021 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     1081 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     1141 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     1201 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     1261 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     1321 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     1381 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     1441 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     1501 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     1561 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     1621 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     1681 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     1741 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     1801 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1861 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1921 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1981 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2041 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2101 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2161 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2221 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2281 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2341 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2401 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2461 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2521 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2581 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2641 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     2701 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     2761 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2821 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2881 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2941 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     3001 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     3061 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3121 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     3181 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     3241 aaccgtcaga tccgctagcg ctaccggact cagatctcga ggccaccatg aagatggaca
     3301 aaaagactat agtttggttt agaagagacc taaggattga ggataatcct gcattagcag
     3361 cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac
     3421 agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct cacttatctc
     3481 aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac acgatttcag
     3541 cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac cacctctatg
     3601 atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa cgtgggatct
     3661 ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac tgcgaaaagg
     3721 gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg tcgattgaat
     3781 ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct gaagcgattt
     3841 gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt
     3901 tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta aatgagttca
     3961 tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg aattctactt
     4021 cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt ttccagtgtg
     4081 cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa gaaagtgcag
     4141 atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt ttcaacttcc
     4201 cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg gatgctgatg
     4261 ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg gatgccggaa
     4321 tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt gtttcaagct
     4381 ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc tgggatacac
     4441 ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct gggagtatcc
     4501 ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc
     4561 cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca actgaatgga
     4621 tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg gaactcggaa
     4681 caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta gctaaagcta
     4741 tttcaagaac ccgtgaagca cagatcatga tcggagcagc agcccgggtc atggtgagca
     4801 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4861 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4921 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4981 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5041 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5101 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5161 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5221 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5281 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5341 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5401 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5461 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagc ggccgcaggg
     5521 ggcgtcgact gggtacccct cctaagaaga agaggaaggt tgcccccacc atggataaaa
     5581 agaagagaaa ggcccccccg accgatgtca gcctggggga cggtccggat ctagacatgg
     5641 cgcagtggga aatgctgcag aatcttgaca gcccctttca ggatcagctg caccagcttt
     5701 actcgcacag cctcctgcct gtggacattc gacagtactt ggctgtctgg attgaagacc
     5761 agaactggca ggaagctgca cttgggagtg atgattccaa ggctaccatg ctattcttcc
     5821 acttcttgga tcagctgaac tatgagtgtg gccgttgcag ccaggaccca gagtccttgt
     5881 tgctgcagca caatttgcgg aaattctgcc gggacattca gcccttttcc caggatccta
     5941 cccagttggc tgagatgatc tttaacctcc ttctggaaga aaaaagaatt ttgatccagg
     6001 ctcagagggc ccaattggaa caaggagagc cagttctcga aacacctgtg gagagccagc
     6061 aacatgagat tgaatcccgg atcctggatt taagggctat gatggagaag ctggtaaaat
     6121 ccatcagcca actgaaagac cagcaggatg tcttctgctt ccgatataag atccaggcca
     6181 aagggaagac accctctctg gacccccatc agaccaaaga gcagaagatt ctgcaggaaa
     6241 ctctcaatga actggacaaa aggagaaagg aggtgctgga tgcctccaaa gcactgctag
     6301 gccgattaac taccctaatc gagctactgc tgccaaagtt ggaggagtgg aaggcccagc
     6361 agcaaaaagc ctgcatcaga gctcccattg accacgggtt ggaacagctg gagacatggt
     6421 tcacagctgg agcaaagctg ttgtttcacc tgaggcagct gctgaaggag ctgaagggac
     6481 tgagttgcct ggttagctat caggatgacc ctctgaccaa aggggtggac ctacgcaacg
     6541 cccaggtcac agagttgcta cagcgtctgc tccacagagc ctttgtggta gaaacccagc
     6601 cctgcatgcc ccaaactccc catcgacccc tcatcctcaa gactggcagc aagttcaccg
     6661 tccgaacaag gctgctggtg agactccagg aaggcaatga gtcactgact gtggaagtct
     6721 ccattgacag gaatcctcct caattacaag gcttccggaa gttcaacatt ctgacttcaa
     6781 accagaaaac tttgaccccc gagaaggggc agagtcaggg tttgatttgg gactttggtt
     6841 acctgactct ggtggagcaa cgttcaggtg gttcaggaaa gggcagcaat aaggggccac
     6901 taggtgtgac agaggaactg cacatcatca gcttcacggt caaatatacc taccagggtc
     6961 tgaagcagga gctgaaaacg gacaccctcc ctgtggtgat tatttccaac atgaaccagc
     7021 tctcaattgc ctgggcttca gttctctggt tcaatttgct cagcccaaac cttcagaacc
     7081 agcagttctt ctccaacccc cccaaggccc cctggagctt gctgggccct gctctcagtt
     7141 ggcagttctc ctcctatgtt ggccgaggcc tcaactcaga ccagctgagc atgctgagaa
     7201 acaagctgtt cgggcagaac tgtaggactg aggatccatt attgtcctgg gctgacttca
     7261 ctaagcgaga gagccctcct ggcaagttac cattctggac atggctggac aaaattctgg
     7321 agttagtaca tgaccacctg aaggatctct ggaatgatgg acgcatcatg ggctttgtga
     7381 gtcggagcca ggagcgccgg ctgctgaaga agaccatgtc tggcaccttt ctactgcgct
     7441 tcagtgaatc gtcagaaggg ggcattacct gctcctgggt ggagcaccag gatgatgaca
     7501 aggtgctcat ctactctgtg caaccgtaca cgaaggaggt gctgcagtca ctcccgctga
     7561 ctgaaatcat ccgccattac cagttgctca ctgaggagaa tatacctgaa aacccactgc
     7621 gcttcctcta tccccgaatc ccccgggatg aagcttttgg gtgctactac caggagaaag
     7681 ttaatctcca ggaacggagg aaatacctga aacacaggct cattgtggtc tctaatagac
     7741 aggtggatga actgcaacaa ccgctggagc ttaagccaga gccagagctg gagtcattag
     7801 agctggaact agggctggtg ccagagccag agctcagcct ggacttagag ccactgctga
     7861 aggcagggct ggatctgggg ccagagctag agtctgtgct ggagtccact ctggagcctg
     7921 tgatagagcc cacactatgc atggtatcac aaacagtgcc agagccagac caaggacctg
     7981 tatcacagcc agtgccagag ccagatttgc cctgtgatct gagacatttg aacactgagc
     8041 caatggaaat cttcagaaac tgtgtaaaga ttgaagaaat catgccgaat ggtgacccac
     8101 tgttggctgg ccagaacacc gtggatgagg tttacgtctc ccgccccagc cacttctaca
     8161 ctgatggacc cttgatgcct tctgacttcc aaccggtcgc caccatggat atctcgatct
     8221 agcggctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8281 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     8341 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8401 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     8461 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     8521 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     8581 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     8641 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     8701 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     8761 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     8821 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     8881 caca
//
