LOCUS       sequence-364133-        4088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-injection fragment for extra-chromosomal array integration at 
            ce-unc-119 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM371 - ce-unc-119 array integration
FEATURES             Location/Qualifiers
     source          1..4088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1466..1482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1466..1482)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1479..1501)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1654..2242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1734..1753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2359..2383)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2483..3274,3275..3343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3106..3125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3344..3448)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3547..3569
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3561..3578
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3562..3578
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgttcggcaa atttgccgtt tgccgagctg ggcaaatttt gagatttgcc gcacacccct
       61 tttatgaacg tgctgacgtc acatcttttt ggacaaagaa ttcccgcatt ttttgtatat
      121 caaaccgtga tgggacagcc tggcaccacg tggggttggc ggcaaatgat tttttccgac
      181 aaatcggcaa attggcaaat tgccggaatt taaagtttct ggcaaattgt tgatttgccg
      241 aaaaaaaatt tcaaaaaggc acagttttaa gtgtttccgt cttataaaat atttctctaa
      301 aaatttccgg aaaacggaaa attgccggaa atacaaagtt tcgtcaaatc ggcaaacctg
      361 caatttgccg aacggcaact gccgccaaca cctggttccc aaaaaagcgt gcactgtttg
      421 aatattaaaa aaaattcaca caaaaataat aaattgaaat aggctaaatt cacatctatg
      481 tacaaaaatt gcatgccagc accggtcaat tttctcaatt actttttgtc tatttttgcc
      541 gaacataatt tgctgtttcc ttggcagaat acagaaaact ctttttttca gatcccacgt
      601 ccacctccaa gtacagaaca aggtatcacc accgaatccg aactggccaa aaaagcgcag
      661 atcaccccga acgacgtgct ggccctgcca ggcattaccc aaggcttcct gtgcagccca
      721 agcgcgaaca tttataacat tgagtttacc aagtttcaga tccgcgatct ggataccgag
      781 caggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcaggtgctg
      841 tttgagattg cgaagccgga aaacgaccag gagaacgatg aatccccgca agaaagcgcc
      901 cgctacgtgc gctaccgctt tgcgccgaac tttctcaaac tcaaaaccgt gggagcgacc
      961 gtcgaattta aggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
     1021 caggtgggcg atattccaat tcatcatttt cgtatgattg aacgccattt ctttaaagat
     1081 cgcctcctga agtgctttga tttcgaattt ggattttgca ttccaaacag ccgtaacaac
     1141 tgcgaacata tctatgagtt tccgcaactg agccagcagc tcatggatga tatgatcaac
     1201 aacccgaacg aaacccgtag cgacagcttt tactttgtcg ataacaaact cgtgatgcac
     1261 aacaaagcgg actatagcta cgatgcgtaa atcttcattg ttgagtttat cttgttgatt
     1321 tttgaataaa ttatcaactc tttacttttt aatgggttat gaaataaata aacattgaaa
     1381 actgataaac aacgttcatc tcttctagaa ggctaggtgg aggctcagtg atgataagtc
     1441 tgcgatggtg gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1501 tcagagggca caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc
     1561 agttcggcgt atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct
     2281 attcaacaaa gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc
     2341 ctcgtgatac caattcggag cctgcttttt tgtacaaact tgttgataat ggcaattcaa
     2401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagagcca cgctcaccgg
     2641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca gatacctgaa
     3481 acaaaaccca tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag
     3541 ggttttccca gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct
     3601 ggaataagga agtgccattc cgcctgacct tctagatgag agagagagcg tgtttatgca
     3661 cgcagtatac taactcctga tatccaattt catatatcga tatgcttgct ttcttttctt
     3721 ctacgttgaa attgtttgaa cttttctcaa aaaacgcgga ttttagagag acttagagtt
     3781 gctccaaata aatttaattt gcaattttgc acttactatt agaatctact tttatagcgc
     3841 taaaatgacc gaataacaca gtagatttta aaagaaaatt ttaaaaatta tttttttgtg
     3901 cagtcaaatt tcgctagttt ttgccactca tttttgaaca tttgtatatg tgcctttcgg
     3961 tggttcaatc aggggtggca cgtggtgtca gagtgttcca tttcggtttg atctacgttg
     4021 atctacaaaa aatgcgggag aagagacgca gagttctcaa ctgatttcac atggttatga
     4081 accagggg
//
