LOCUS       sequence-364134-        9675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains cDNA of wt MET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Cerqua M, Botti O, Arigoni M, Gioelli N, Serini G, Calogero R, 
            Boccaccio C, Comoglio PM, Altintas DM
  TITLE     MET14 promotes a ligand-dependent, AKT-driven invasive growth.
  JOURNAL   Life Sci Alliance. 2022 May 30;5(10). pii: 5/10/e202201409. doi: 
            10.26508/lsa.202201409. Print 2022 Oct.
  PUBMED    35636967
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-05-30"; volume: "5(10). pii: 
            5/10/e202201409. doi"; pages: " 10.26508/lsa.202201409. Print 2022 
            Oct"
COMMENT     Sequence Label: pCDNA_MET_3Flag
FEATURES             Location/Qualifiers
     source          1..9675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        295..674
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        675..878
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     829..849
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     923..942
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        923..941
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5152..5229
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKGDYKDDDDKIDYKDDDDK"
     primer_bind     complement(5326..5343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5332..5556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5602..6030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5689..5708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5899..5920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6039..6059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6044..6373
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6224..6359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6286..6305
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6440..7234
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6494..6513)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7104..7123
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7408..7529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7445..7464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7499..7518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7578..7594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7578..7594)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7591..7613)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7602..7618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7626..7632,7633..7650,7651..7656))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7671..7692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7809..7826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7980..8568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8060..8079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8739..9530,9531..9599))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9362..9381
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9600..9675,1..29))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
       61 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
      121 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      181 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      241 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      301 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      361 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      421 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      481 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      541 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      601 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      661 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      721 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      781 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      841 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      901 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      961 atgaaggccc ccgctgtgct tgcacctggc atcctcgtgc tcctgtttac cttggtgcag
     1021 aggagcaatg gggagtgtaa agaggcacta gcaaagtccg agatgaatgt gaatatgaag
     1081 tatcagcttc ccaacttcac cgcggaaaca cccatccaga atgtcattct acatgagcat
     1141 cacattttcc ttggtgccac taactacatt tatgttttaa atgaggaaga ccttcagaag
     1201 gttgctgagt acaagactgg gcctgtgctg gaacacccag attgtttccc atgtcaggac
     1261 tgcagcagca aagccaattt atcaggaggt gtttggaaag ataacatcaa catggctcta
     1321 gttgtcgaca cctactatga tgatcaactc attagctgtg gcagcgtcaa cagagggacc
     1381 tgccagcgac atgtctttcc ccacaatcat actgctgaca tacagtcgga ggttcactgc
     1441 atattctccc cacagataga agagcccagc cagtgtcctg actgtgtggt gagcgccctg
     1501 ggagccaaag tcctttcatc tgtaaaggac cggttcatca acttctttgt aggcaatacc
     1561 ataaattctt cttatttccc agatcatcca ttgcattcga tatcagtgag aaggctaaag
     1621 gaaacgaaag atggttttat gtttttgacg gaccagtcct acattgatgt tttacctgag
     1681 ttcagagatt cttaccccat taagtatgtc catgcctttg aaagcaacaa ttttatttac
     1741 ttcttgacgg tccaaaggga aactctagat gctcagactt ttcacacaag aataatcagg
     1801 ttctgttcca taaactctgg attgcattcc tacatggaaa tgcctctgga gtgtattctc
     1861 acagaaaaga gaaaaaagag atccacaaag aaggaagtgt ttaatatact tcaggctgcg
     1921 tatgtcagca agcctggggc ccagcttgct agacaaatag gagccagcct gaatgatgac
     1981 attcttttcg gggtgttcgc acaaagcaag ccagattctg ccgaaccaat ggatcgatct
     2041 gccatgtgtg cattccctat caaatatgtc aacgacttct tcaacaagat cgtcaacaaa
     2101 aacaatgtga gatgtctcca gcatttttac ggacccaatc atgagcactg ctttaatagg
     2161 acacttctga gaaattcatc aggctgtgaa gcgcgccgtg atgaatatcg aacagagttt
     2221 accacagctt tgcagcgcgt tgacttattc atgggtcaat tcagcgaagt cctcttaaca
     2281 tctatatcca ccttcattaa aggagacctc accatagcta atcttgggac atcagagggt
     2341 cgcttcatgc aggttgtggt ttctcgatca ggaccatcaa cccctcatgt gaattttctc
     2401 ctggactccc atccagtgtc tccagaagtg attgtggagc atacattaaa ccaaaatggc
     2461 tacacactgg ttatcactgg gaagaagatc acgaagatcc cattgaatgg cttgggctgc
     2521 agacatttcc agtcctgcag tcaatgcctc tctgccccac cctttgttca gtgtggctgg
     2581 tgccacgaca aatgtgtgcg atcggaggaa tgcctgagcg ggacatggac tcaacagatc
     2641 tgtctgcctg caatctacaa ggttttccca aatagtgcac cccttgaagg agggacaagg
     2701 ctgaccatat gtggctggga ctttggattt cggaggaata ataaatttga tttaaagaaa
     2761 actagagttc tccttggaaa tgagagctgc accttgactt taagtgagag cacgatgaat
     2821 acattgaaat gcacagttgg tcctgccatg aataagcatt tcaatatgtc cataattatt
     2881 tcaaatggcc acgggacaac acaatacagt acattctcct atgtggatcc tgtaataaca
     2941 agtatttcgc cgaaatacgg tcctatggct ggtggcactt tacttacttt aactggaaat
     3001 tacctaaaca gtgggaattc tagacacatt tcaattggtg gaaaaacatg tactttaaaa
     3061 agtgtgtcaa acagtattct tgaatgttat accccagccc aaaccatttc aactgagttt
     3121 gctgttaaat tgaaaattga cttagccaac cgagagacaa gcatcttcag ttaccgtgaa
     3181 gatcccattg tctatgaaat tcatccaacc aaatctttta ttagtggtgg gagcacaata
     3241 acaggtgttg ggaaaaacct gaattcagtt agtgtcccga gaatggtcat aaatgtgcat
     3301 gaagcaggaa ggaactttac agtggcatgt caacatcgct ctaattcaga gataatctgt
     3361 tgtaccactc cttccctgca acagctgaat ctgcaactcc ccctgaaaac caaagccttt
     3421 ttcatgttag atgggatcct ttccaaatac tttgatctca tttatgtaca taatcctgtg
     3481 tttaagcctt ttgaaaagcc agtgatgatc tcaatgggca atgaaaatgt actggaaatt
     3541 aagggaaatg atattgaccc tgaagcagtt aaaggtgaag tgttaaaagt tggaaataag
     3601 agctgtgaga atatacactt acattctgaa gccgttttat gcacggtccc caatgacctg
     3661 ctgaaattga acagcgagct aaatatagag tggaagcaag caatttcttc aaccgtcctt
     3721 ggaaaagtaa tagttcaacc agatcagaat ttcacaggat tgattgctgg tgttgtctca
     3781 atatcaacag cactgttatt actacttggg tttttcctgt ggctgaaaaa gagaaagcaa
     3841 attaaagatc tgggcagtga attagttcgc tacgatgcaa gagtacacac tcctcatttg
     3901 gataggcttg taagtgcccg aagtgtaagc ccaactacag aaatggtttc aaatgaatct
     3961 gtagactacc gagctacttt tccagaagat cagtttccta attcatctca gaacggttca
     4021 tgccgacaag tgcagtatcc tctgacagac atgtccccca tcctaactag tggggactct
     4081 gatatatcca gtccattact gcaaaatact gtccacattg acctcagtgc tctaaatcca
     4141 gagctggtcc aggcagtgca gcatgtagtg attgggccca gtagcctgat tgtgcatttc
     4201 aatgaagtca taggaagagg gcattttggt tgtgtatatc atgggacttt gttggacaat
     4261 gatggcaaga aaattcactg tgctgtgaaa tccttgaaca gaatcactga cataggagaa
     4321 gtttcccaat ttctgaccga gggaatcatc atgaaagatt ttagtcatcc caatgtcctc
     4381 tcgctcctgg gaatctgcct gcgaagtgaa gggtctccgc tggtggtcct accatacatg
     4441 aaacatggag atcttcgaaa tttcattcga aatgagactc ataatccaac tgtaaaagat
     4501 cttattggct ttggtcttca agtagccaaa ggcatgaaat atcttgcaag caaaaagttt
     4561 gtccacagag acttggctgc aagaaactgt atgctggatg aaaaattcac agtcaaggtt
     4621 gctgattttg gtcttgccag agacatgtat gataaagaat actatagtgt acacaacaaa
     4681 acaggtgcaa agctgccagt gaagtggatg gctttggaaa gtctgcaaac tcaaaagttt
     4741 accaccaagt cagatgtgtg gtcctttggc gtgctcctct gggagctgat gacaagagga
     4801 gccccacctt atcctgatgt aaacaccttt gatataactg tttacttgtt gcaagggaga
     4861 agactcctac aacccgaata ctgcccagac cccttatatg aagtaatgct aaaatgctgg
     4921 caccctaaag ccgaaatgcg cccatccttt tctgaactgg tgtcccggat atcagcaatc
     4981 ttctctactt tcattgggga gcactatgtc catgtgaacg ctacttatgt gaacgtaaaa
     5041 tgtgtcgctc catatccttc tctgttgtca tcagaagata acgctgatga tgaggtggac
     5101 acacgaccag cctccttctg ggagacatca aagcttggcg gtgggggtgg cgattacaag
     5161 gatgacgacg ataagggaga ttacaaggat gacgacgata agatcgatta caaggatgac
     5221 gacgataagt aaggatccac tagtccagtg tggtggaatt ctgcagatat ccagcacagt
     5281 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     5341 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5401 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5461 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5521 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5581 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5641 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5701 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5761 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5821 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5881 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5941 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6001 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     6061 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6121 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6181 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6241 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6301 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6361 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     6421 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     6481 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6541 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6601 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6661 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6721 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6781 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6841 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6901 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6961 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7021 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7081 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7141 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7201 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     7261 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     7321 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7381 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7501 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7561 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7621 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7681 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7741 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7801 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7861 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8821 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8881 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8941 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9001 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9061 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9661 atatttgaat gtatt
//
