LOCUS       sequence-364135-        9534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains cDNA of the MET gene without the exon 14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Cerqua M, Botti O, Arigoni M, Gioelli N, Serini G, Calogero R, 
            Boccaccio C, Comoglio PM, Altintas DM
  TITLE     MET14 promotes a ligand-dependent, AKT-driven invasive growth.
  JOURNAL   Life Sci Alliance. 2022 May 30;5(10). pii: 5/10/e202201409. doi: 
            10.26508/lsa.202201409. Print 2022 Oct.
  PUBMED    35636967
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-05-30"; volume: "5(10). pii: 
            5/10/e202201409. doi"; pages: " 10.26508/lsa.202201409. Print 2022 
            Oct"
COMMENT     Sequence Label: pCDNA_MET_D14_3Flag
FEATURES             Location/Qualifiers
     source          1..9534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(95..114)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        286..665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        666..869
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     820..840
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     914..933
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        914..932
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5002..5079
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKGDYKDDDDKIDYKDDDDK"
     primer_bind     complement(5176..5193)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5182..5406
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5452..5880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5539..5558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5749..5770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5889..5909)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5894..6223
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6074..6209
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6136..6155
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6290..7084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6344..6363)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6954..6973
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7258..7379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7295..7314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7349..7368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7428..7444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7428..7444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7441..7463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7452..7468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7476..7482,7483..7500,7501..7506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7521..7542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7659..7676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7830..8418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7910..7929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8589..9380,9381..9449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9212..9231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9450..9534,1..20))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
       61 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      121 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
      181 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
      241 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
      301 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      361 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      421 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      481 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      541 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      601 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      661 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      721 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      781 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      841 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
      901 ggcttatcga aattaatacg actcactata gggagaccca agctggctag catgaaggcc
      961 cccgctgtgc ttgcacctgg catcctcgtg ctcctgttta ccttggtgca gaggagcaat
     1021 ggggagtgta aagaggcact agcaaagtcc gagatgaatg tgaatatgaa gtatcagctt
     1081 cccaacttca ccgcggaaac acccatccag aatgtcattc tacatgagca tcacattttc
     1141 cttggtgcca ctaactacat ttatgtttta aatgaggaag accttcagaa ggttgctgag
     1201 tacaagactg ggcctgtgct ggaacaccca gattgtttcc catgtcagga ctgcagcagc
     1261 aaagccaatt tatcaggagg tgtttggaaa gataacatca acatggctct agttgtcgac
     1321 acctactatg atgatcaact cattagctgt ggcagcgtca acagagggac ctgccagcga
     1381 catgtctttc cccacaatca tactgctgac atacagtcgg aggttcactg catattctcc
     1441 ccacagatag aagagcccag ccagtgtcct gactgtgtgg tgagcgccct gggagccaaa
     1501 gtcctttcat ctgtaaagga ccggttcatc aacttctttg taggcaatac cataaattct
     1561 tcttatttcc cagatcatcc attgcattcg atatcagtga gaaggctaaa ggaaacgaaa
     1621 gatggtttta tgtttttgac ggaccagtcc tacattgatg ttttacctga gttcagagat
     1681 tcttacccca ttaagtatgt ccatgccttt gaaagcaaca attttattta cttcttgacg
     1741 gtccaaaggg aaactctaga tgctcagact tttcacacaa gaataatcag gttctgttcc
     1801 ataaactctg gattgcattc ctacatggaa atgcctctgg agtgtattct cacagaaaag
     1861 agaaaaaaga gatccacaaa gaaggaagtg tttaatatac ttcaggctgc gtatgtcagc
     1921 aagcctgggg cccagcttgc tagacaaata ggagccagcc tgaatgatga cattcttttc
     1981 ggggtgttcg cacaaagcaa gccagattct gccgaaccaa tggatcgatc tgccatgtgt
     2041 gcattcccta tcaaatatgt caacgacttc ttcaacaaga tcgtcaacaa aaacaatgtg
     2101 agatgtctcc agcattttta cggacccaat catgagcact gctttaatag gacacttctg
     2161 agaaattcat caggctgtga agcgcgccgt gatgaatatc gaacagagtt taccacagct
     2221 ttgcagcgcg ttgacttatt catgggtcaa ttcagcgaag tcctcttaac atctatatcc
     2281 accttcatta aaggagacct caccatagct aatcttggga catcagaggg tcgcttcatg
     2341 caggttgtgg tttctcgatc aggaccatca acccctcatg tgaattttct cctggactcc
     2401 catccagtgt ctccagaagt gattgtggag catacattaa accaaaatgg ctacacactg
     2461 gttatcactg ggaagaagat cacgaagatc ccattgaatg gcttgggctg cagacatttc
     2521 cagtcctgca gtcaatgcct ctctgcccca ccctttgttc agtgtggctg gtgccacgac
     2581 aaatgtgtgc gatcggagga atgcctgagc gggacatgga ctcaacagat ctgtctgcct
     2641 gcaatctaca aggttttccc aaatagtgca ccccttgaag gagggacaag gctgaccata
     2701 tgtggctggg actttggatt tcggaggaat aataaatttg atttaaagaa aactagagtt
     2761 ctccttggaa atgagagctg caccttgact ttaagtgaga gcacgatgaa tacattgaaa
     2821 tgcacagttg gtcctgccat gaataagcat ttcaatatgt ccataattat ttcaaatggc
     2881 cacgggacaa cacaatacag tacattctcc tatgtggatc ctgtaataac aagtatttcg
     2941 ccgaaatacg gtcctatggc tggtggcact ttacttactt taactggaaa ttacctaaac
     3001 agtgggaatt ctagacacat ttcaattggt ggaaaaacat gtactttaaa aagtgtgtca
     3061 aacagtattc ttgaatgtta taccccagcc caaaccattt caactgagtt tgctgttaaa
     3121 ttgaaaattg acttagccaa ccgagagaca agcatcttca gttaccgtga agatcccatt
     3181 gtctatgaaa ttcatccaac caaatctttt attagtggtg ggagcacaat aacaggtgtt
     3241 gggaaaaacc tgaattcagt tagtgtcccg agaatggtca taaatgtgca tgaagcagga
     3301 aggaacttta cagtggcatg tcaacatcgc tctaattcag agataatctg ttgtaccact
     3361 ccttccctgc aacagctgaa tctgcaactc cccctgaaaa ccaaagcctt tttcatgtta
     3421 gatgggatcc tttccaaata ctttgatctc atttatgtac ataatcctgt gtttaagcct
     3481 tttgaaaagc cagtgatgat ctcaatgggc aatgaaaatg tactggaaat taagggaaat
     3541 gatattgacc ctgaagcagt taaaggtgaa gtgttaaaag ttggaaataa gagctgtgag
     3601 aatatacact tacattctga agccgtttta tgcacggtcc ccaatgacct gctgaaattg
     3661 aacagcgagc taaatataga gtggaagcaa gcaatttctt caaccgtcct tggaaaagta
     3721 atagttcaac cagatcagaa tttcacagga ttgattgctg gtgttgtctc aatatcaaca
     3781 gcactgttat tactacttgg gtttttcctg tggctgaaaa agagaaagca aattaaagat
     3841 cagtttccta attcatctca gaacggttca tgccgacaag tgcagtatcc tctgacagac
     3901 atgtccccca tcctaactag tggggactct gatatatcca gtccattact gcaaaatact
     3961 gtccacattg acctcagtgc tctaaatcca gagctggtcc aggcagtgca gcatgtagtg
     4021 attgggccca gtagcctgat tgtgcatttc aatgaagtca taggaagagg gcattttggt
     4081 tgtgtatatc atgggacttt gttggacaat gatggcaaga aaattcactg tgctgtgaaa
     4141 tccttgaaca gaatcactga cataggagaa gtttcccaat ttctgaccga gggaatcatc
     4201 atgaaagatt ttagtcatcc caatgtcctc tcgctcctgg gaatctgcct gcgaagtgaa
     4261 gggtctccgc tggtggtcct accatacatg aaacatggag atcttcgaaa tttcattcga
     4321 aatgagactc ataatccaac tgtaaaagat cttattggct ttggtcttca agtagccaaa
     4381 ggcatgaaat atcttgcaag caaaaagttt gtccacagag acttggctgc aagaaactgt
     4441 atgctggatg aaaaattcac agtcaaggtt gctgattttg gtcttgccag agacatgtat
     4501 gataaagaat actatagtgt acacaacaaa acaggtgcaa agctgccagt gaagtggatg
     4561 gctttggaaa gtctgcaaac tcaaaagttt accaccaagt cagatgtgtg gtcctttggc
     4621 gtgctcctct gggagctgat gacaagagga gccccacctt atcctgacgt aaacaccttt
     4681 gatataactg tttacttgtt gcaagggaga agactcctac aacccgaata ctgcccagac
     4741 cccttatatg aagtaatgct aaaatgctgg caccctaaag ccgaaatgcg cccatccttt
     4801 tctgaactgg tgtcccggat atcagcgagc ttctctactt tcattgggga gcactatgtc
     4861 catgtgaacg ctacttatgt gaacgtaaaa tgtgtcgctc cgtatccttc tctgttgtca
     4921 tcagaagata acgctgatga tgaggtggac acacgaccag cctccttctg ggagacatca
     4981 aagcttggcg gtgggggtgg cgattacaag gatgacgacg ataagggaga ttacaaggat
     5041 gacgacgata agatcgatta caaggatgac gacgataagt aaggatccac tagtccagtg
     5101 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt
     5161 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5221 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5281 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5341 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5401 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     5461 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5521 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5581 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5641 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5701 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5761 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5821 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5881 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5941 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6001 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6061 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6121 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     6181 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     6241 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     6301 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6361 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6421 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6481 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6541 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6601 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6661 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6721 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6781 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6841 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6901 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6961 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7021 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7081 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     7141 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7201 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     7261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7381 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7441 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7501 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7561 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7621 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7681 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7741 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7801 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7861 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7921 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7981 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8041 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8101 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8161 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8221 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8281 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8341 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8401 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8461 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8521 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8581 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8641 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8701 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8761 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8821 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8881 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8941 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9001 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9061 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9121 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9181 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9241 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9301 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9361 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9421 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9481 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaat
//
