LOCUS       sequence-36415-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A
  TITLE     Biochemical analysis of the canonical model for the mammalian 
            circadian clock.
  JOURNAL   J Biol Chem. 2011 Jul 22;286(29):25891-902. doi: 
            10.1074/jbc.M111.254680. Epub 2011 May 25.
  PUBMED    21613214
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.254680"; journalName: "J Biol Chem"; date: 
            "2011-07-22- 22"; volume: "286"; issue: "29"; pages: "25891-902"
COMMENT     Sequence Label: pCKFB1
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             142..162
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             175..198
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgtttcgtac agttttgtat aaaaaaacct ataaatattc cggattattc ataccgtccc
       61 accatcgggc gcggatctcg gtccgaaacc atgtcgtact accatcacca tcaccatcac
      121 gattacgata tcccaacgac cgaaaacctg tattttcagg gcgccatgga tccggactac
      181 aaagacgatg acgacaagat ggtgtttacc gtaagctgta gtaaaatgag ctcaattgtt
      241 gacagagatg acagtagtat ttttgatgga ttggtggaag aagatgacaa ggacaaagca
      301 aaaagagtat ctagaaacaa atcagaaaag aaacgtagag atcagttcaa tgtcctcatt
      361 aaggagctgg ggtctatgct tcctggtaac gcgagaaaga tggacaagtc tactgttcta
      421 cagaagagca ttgatttttt gcgcaaacat aaagagacca ctgcacagtc agatgctagt
      481 gagattcgac aggactggaa acccacattc cttagtaatg aagagtttac acagttaatg
      541 ttagaggctc ttgatggttt ttttttagcg atcatgacag atggaagtat aatatatgta
      601 tctgagagtg taacttcgtt acttgaacat ttaccatctg atcttgtgga tcaaagtata
      661 tttaatttta tcccagaggg agaacattca gaggtttata agatactctc tactcatctg
      721 ctggaaagtg actcattaac ccctgagtac ttaaaatcaa aaaatcagtt agaattctgt
      781 tgtcacatgc ttcgaggaac aatagaccca aaggagccat ccacctatga atatgtgaga
      841 tttataggaa a
//
