LOCUS       sequence-364150-        8989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional co-activator BRD4 labeled by mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
  TITLE     Transcription activation is enhanced by multivalent interactions 
            independent of phase separation.
  JOURNAL   Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017.
  PUBMED    35537448
REFERENCE   2  (bases 1 to 8989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017."
COMMENT     Sequence Label: mCherry-BRD4
FEATURES             Location/Qualifiers
     source          1..8989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(862..879)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3142..3849)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3417..3436)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3686..3704
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(3888..4091)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3917..3937)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4092..4395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4570..5158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4650..4669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5487..5534
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5559..5578
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5766..6560)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5766..6560)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5877..5896)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6487..6506
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6595..6952)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6609..6744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6761..6952
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6909..6929
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(6954..7058)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7085..7540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7172..7191)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7382..7403
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    7547..7668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7558..7577)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7612..7631
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gggctgctgt cctgggggtg ggtgagtagg ttgctgtcct gggggtggtg ggggctgctg
       61 aatatgagca gaaaagggca tggatggcaa gtgaactggt cgtggtggag gctggtgttg
      121 ttgctggggt ggtggctgtg gctgaggagg tgggggtggc ggtggctgtg gctggagctg
      181 ctgctgctgc acagaagggt ggggtggagg cggcaaaggc ggtgggggtt gggactgcac
      241 cttcacggaa gggagtagtg gtgtgggagg ctgcaccttc tgcagctgct gtaggtacaa
      301 ctgcatctgc atggaggtga ggggtggggc aggtggctct tcatcctcca gcagcacttg
      361 ggggggctga gccattggtg gttgtgggag cagggggggc tgggccaggg caggggacac
      421 agctgggggt cgggtaggct ttgggggcag agcagcagct cggttactgg gccgagatgg
      481 ctgttggggc agcgcattgt gcaaagctgg aggagagacc acagcatgct ggttgagatg
      541 gggtggagtg ctgtgctcag gtggctgggg caggtgagga ggcagctccg gctgcggcag
      601 gtgcaagatg ggctgggtga agtggccaat agggtcaaag acactgcctg gcagctgtgg
      661 ttccaggacg gggacctggg cagtgatgaa gggtgggggc gaggacttca tcgctggggc
      721 agtctgctgt ggcatggagg gcggaggtgg tgggggtgga ggttgctgct gctgctgctg
      781 cggaggtggg ggtggaggag gctgctgtgg aggtgggggc ggctgctggg gcacaggagc
      841 tggggctggc tgcatctgtg gatggtgatg gtgatgatgc tttttctggt ccctcccagt
      901 gtgccccttc ttttttgact tgggagccat ctctgtttca gagtcttcac tgtcagagga
      961 gctggattcg ctggtgctct ccgactcaga ggatgagaat cccttcatct tggaagaacc
     1021 agcaatcacg tcaacttttt cagcttgagg tttccttttc ttccgcaaac aggaggtgac
     1081 atatcgctcc aactctcgta gtgtagatgg cttcagggtc tcaaagtcaa tctcaatctc
     1141 atcggggttg gagtttttaa gtgatggttc ccttgactga attatgtgta ctacacggcc
     1201 tagcttctca ccaggaagtt tgttgatatc tagacttagc tgccgcttct cctcataaga
     1261 catgggctta cacttatcct cctcttctga ttcatatgtg ggaggcggct tggtcttcgt
     1321 gggtactggt tccttcttgc tcacattgct gttgctgctg ttatttttct ttgtcttttt
     1381 gggaggaagt tccttggttt tgcttttttt attttcctcc acttcttctt tctttttgtg
     1441 cttttccttt ttcttttcct tcttgtcctt ctcctttttc tttggtttgt tctgctgggg
     1501 ctgtgagagg gctgcaagct gctcatgcac ggccttgagc tgttcctgga gttcagccag
     1561 ccgctgggct cgctcttcct cagagtcgtc agtggaactg tcgctgtcgg aagaactgtc
     1621 gctgctgctg tcactagatg agggtggggc taccaccttt gtagggggtg gcactgcagg
     1681 agaggacact gtaacaactg gctcttcagg ctcatcaggc atcttggcaa agcgcatttc
     1741 aaacacatcc tggagttttc gagccatggc taccacttca tggtcagggg ggttgtactt
     1801 gtagcagttg gagaacatca atcggacatc agcaccaaat tcctgggcat ctctgtactc
     1861 tcgggactct agtttagact tgattgtgct catgtccatg ggatgtttga tgatgtcaca
     1921 gtagtcgtgc agacccagtg cctccacatc cacaggcttg tagaaaggcc aggcataggc
     1981 agcatgtttc ttggcaaaca tctccttgag gatgccactg cagcacttta gctgctcaga
     2041 gatcttgctg ctcttctctg gccctgggtg ctgctgtgag tccggtacat ccttctttgg
     2101 aggcttcaca ggtctgctgc tctcccgccg aggacccagc ttggcggtct tgggctctgg
     2161 ggccagtgag ggtggctcat gaatggggtc gatggtggta ggggtggtgg tatctgcttt
     2221 cctcttcacc cctttctttg tcttcacagg ctggggggtg gtcgcaatga tgggcgggtg
     2281 actctgcaca ggctgtggaa ctggagcagg tgggggtggt ggctgggggg gtaccggtga
     2341 aggagtctga agtggctgag ggggcaccat tgtcatgaca ggaggctggg caatgaggtc
     2401 tggggtgaca gcaggaaagg ggtgagttgt ggcctgcaca ggtggaggag ggttctgctg
     2461 aggcgtctgg gtctgcggag aagttgatgc ttgagttgtg tttggtaccg tggatacacc
     2521 aggctttgct gcccctgttt ctttcctccc tcgtcctctt ccttttgcct ggactatcat
     2581 gatctcagtt tcttctgtag gcagttcatt gattttttgc aagaagagct tctccagagc
     2641 ttctgccatt aagacgatgt catctccagg cttgttatag atgtaacaat ttgtaaacat
     2701 agtgttgaag tcctggatac attcctgagc attccagtaa tagttgtttt ccaagcgctt
     2761 ctttattgtt cccatatcca tgggtgtttt aataatctta tagtaatcag ggaggttcag
     2821 cttgacggca tccacgggct gctggaaagg ccacgcaaac tggtgtttcc atagtgtctt
     2881 gagtaccact ctgagcagat attgcagttg gtttgtctgt ctcttgggct tgttagggtt
     2941 ggaggtctct gggggtggag gattggtgct ggctgcattt gctggctggg gttgggcctg
     3001 ggcctgcgtt gtagacattt gggaggtttc tagtccatcc cccattactg gcagatttct
     3061 caatcttgtc ccagggccgc tctccgtaga catgctagtg gatccggaat tcgaagcttg
     3121 agctcgagat ctgagtccgg acttgtacag ctcgtccatg ccgccggtgg agtggcggcc
     3181 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
     3241 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
     3301 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
     3361 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
     3421 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
     3481 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
     3541 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
     3601 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
     3661 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
     3721 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
     3781 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
     3841 gctcaccatt accggtagcg ctagcggatc tgacggttca ctaaaccagc tctgcttata
     3901 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
     3961 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
     4021 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     4081 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     4141 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     4201 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     4261 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     4321 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     4381 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     4441 tactattaat aactaatgca tggcggtaat acggttatcc acagaatcag gggataacgc
     4501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4981 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5221 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5281 aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc
     5341 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     5401 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     5461 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg
     5521 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     5581 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     5641 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     5701 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
     5761 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     5821 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     5881 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
     5941 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     6001 gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct
     6061 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     6121 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     6181 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     6241 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     6301 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     6361 agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     6421 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     6481 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     6541 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca
     6601 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     6661 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     6721 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     6781 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     6841 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     6901 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc
     6961 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7021 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7081 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7141 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7201 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7261 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7321 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7381 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7441 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7501 tttaacgcga attttaacaa aatattaacg cttacaattt acgcgttaag atacattgat
     7561 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     7621 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     7681 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     7741 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tggatcccgg
     7801 gcccgcggta ccgtcgactg cagaattccg tcgaggtcaa catttttgcc agaaaaagtc
     7861 agaagtcacc tgggtgctct caaaaaagat tttcttcaaa tattgacaaa agatcactct
     7921 ggaaattcat gtcaattgta gctgccattg cctccctgcg cctccgctcc tgctctcgtt
     7981 tccgggccaa ctccctctgc tggtccagca tagactgggg ctgggagctc tgggcctggg
     8041 gggcagaggc agcggccaca gcagctgcct gctgctgctg ctgctgctgc tcctgtctct
     8101 gctgctgctg ctgctgctgc tgctcctggc gccgtcgtgc ctcctcatgg gctcgacgtg
     8161 cctgttccag cgcatcttca tcctctcggc tcctcatgcg ctcctgcctc agccgctcct
     8221 tctccttctc tgcatgctca gcctgagcct tcagggcctt ttccctctcc tccttctccc
     8281 gagcagcacg gcggaaatgc tcaaagctgt cacttgagga cttggctgtg gaggatgggg
     8341 tggtcggatg cttctgtacc aggctggccc aggagcccat gttcttaatt ttcaggtcct
     8401 ttttgggtgc cactggtgtt tttggctcct gcttctgttt gtccttgtca ggggcccctg
     8461 gtgggggtgc actctgctct ggaggccgga ttacaggcct ccctatgtct acaggcttca
     8521 tctcaggccg ctggggcagg tggactgggg ccttgatgtt ctctgggtgc ttggggggct
     8581 ctggtcgaag tgaggtgctg aaaggctcgc tgcgaatgat tggtgagtga atcttctcct
     8641 cctttactac caccaggggc tggggctgga cgactgaagg tggtcgcagc tcctgggaca
     8701 gcataggcac ggcagccggt gaggcaggtg ggcatccctg gccttggccc atgactggcc
     8761 ctggtggctg tggctcagcc ctgcccttta cctgcttctt aggctggacg ttttgctggg
     8821 gaggagactg atgggtcagg ctctggaact gtggcatctg aggggaatgt atcataagcg
     8881 gagagggagc ctcacgaaga tgaccagctg agtaggggtc tgacttgtgg tgccgggggg
     8941 aagggtgatg ctggatgact tgctggggct tggcaggctg tggtggtgg
//
