LOCUS       sequence-364152-       10232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ICUE4 cAMP sensor tethered to the PKA RI-alpha subunit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke 
            M, Rangamani P, Taylor SS, Mehta S, Zhang J
  TITLE     Phase Separation of a PKA Regulatory Subunit Controls cAMP 
            Compartmentation and Oncogenic Signaling.
  JOURNAL   Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 
            10.1016/j.cell.2020.07.043. Epub 2020 Aug 25.
  PUBMED    32846158
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07.043"; journalName: "Cell"; date: 
            "2020-09-17- 17"; volume: "182"; issue: "6"; pages: "1531-1544.e15"
COMMENT     Sequence Label: pcDNA3-RI-alpha-ICUE4
FEATURES             Location/Qualifiers
     source          1..10232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      889..898
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2056..2058,2059..2061,2062..2772)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(2056..2058,2059..2061,2062..2772)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(2056..2058,2059..2061,2062..2772)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(5137..5139,5140..5142,5143..5655)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     promoter        complement(5785..5803)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5786..5803)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5823..5840)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5829..6053
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6099..6527
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6186..6205)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6396..6417
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6536..6556)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6541..6870
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6721..6856
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6783..6802
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6937..7731
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6991..7010)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7601..7620
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7905..8026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7942..7961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7996..8015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8075..8091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8075..8091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8088..8110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8099..8115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8123..8129,8130..8147,8148..8153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8168..8189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8306..8323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8477..9065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8557..8576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9236..10027,10028..10096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9859..9878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10097..10201)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccgc caccatggag
      901 tctggcagta ccgccgccag tgaggaggca cgcagccttc gagaatgtga gctctacgtc
      961 cagaagcata acattcaagc actgctcaaa gattctattg tgcagttgtg cactgctcga
     1021 cctgagagac ccatggcatt cctcagggaa tactttgaga ggttggagaa ggaggaggca
     1081 aaacagattc agaatctgca gaaagcaggc actcgtacag actcaaggga ggatgagatt
     1141 tctcctcctc cacccaaccc agtggttaaa ggtaggaggc gacgaggtgc tatcagcgct
     1201 gaggtctaca cggaggaaga tgcggcatcc tatgttagaa aggttatacc aaaagattac
     1261 aagacaatgg ccgctttagc caaagccatt gaaaagaatg tgctgttttc acatcttgat
     1321 gataatgaga gaagtgatat ttttgatgcc atgttttcgg tctcctttat cgcaggagag
     1381 actgtgattc agcaaggtga tgaaggggat aacttctatg tgattgatca aggagagacg
     1441 gatgtctatg ttaacaatga atgggcaacc agtgttgggg aaggagggag ctttggagaa
     1501 cttgctttga tttatggaac accgagagca gccactgtca aagcaaagac aaatgtgaaa
     1561 ttgtggggca tcgaccgaga cagctataga agaatcctca tgggaagcac actgagaaag
     1621 cggaagatgt atgaggaatt ccttagtaaa gtctctattt tagagtctct ggacaagtgg
     1681 gaacgtctta cggtagctga tgcattggaa ccagtgcagt ttgaagatgg gcagaagatt
     1741 gtggtgcagg gagaaccagg ggatgagttc ttcattattt tagaggggtc agctgctgtg
     1801 ctacaacgtc ggtcagaaaa tgaagagttt gttgaagtgg gaagattggg gccttctgat
     1861 tattttggtg aaattgcact actgatgaat cgtcctcgtg ctgccacagt tgttgctcgt
     1921 ggccccttga agtgcgttaa gctggaccga cctagatttg aacgtgttct tggcccatgc
     1981 tcagacatcc tcaaacgaaa catccagcag tacaacagtt ttgtgtcact gtctgtcggc
     2041 ggtactggtg gttccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2101 ctggtcgagc tggacggcga cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag
     2161 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2221 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
     2281 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2341 gagcgtacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2401 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2461 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
     2521 gacaagcaga agaacggcat caaggcccac ttcaagatcc gccacaacat cgaggacggc
     2581 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2641 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     2701 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac
     2761 gagctgtaca aggctggtac ccccgtggga actcatgaga tggaggagga gttggccgaa
     2821 gctgtggccc tgctctccca gcgggggcct gacgccctgc tcactgtggc acttcgaaag
     2881 cccccaggtc agcgcacgga tgaagagctg gacctcatct ttgaggagct gctgcacatc
     2941 aaggctgtgg cccacctctc caactcggtg aagcgagaat tagcggctgt tctgctcttt
     3001 gaaccacaca gcaaggcagg gaccgtgttg ttcagccagg gggacaaggg cacttcgtgg
     3061 tacattatct ggaagggatc tgtcaacgtg gtgacccatg gcaaggggct ggtgaccacc
     3121 ctgcatgagg gagatgattt tggagaactg gctctggtga atgatgcacc ccgggcagcc
     3181 accatcatcc tgcgagaaga caactgtcat ttcctgcgtg tggacaagca ggacttcaac
     3241 cgtatcatca aggatgtgga ggcaaagacc atgcggctgg aagaacatgg caaagtggtg
     3301 ctggtgctgg agagagcctc tcagggcgcc ggcccttccc gacccccaac cccaggcagg
     3361 aaccggtata cagtgatgtc tggcacccca gagaagatcc tagagcttct gttggaggcc
     3421 atgggaccag attccagtgc tcatgaccaa acagagacat tcctcagcga cttcctcctg
     3481 acccacaggg tcttcatgcc cagcgcccaa ctctgcgctg cccttctgca ccacttccat
     3541 gtggagcctg cgggtggcag cgagcaggag cgcagcacct acgtctgcaa caagaggcag
     3601 cagatcttgc ggctggtcag ccagtgggtg gccctgtatg gctccatgct ccacactgac
     3661 cctgtggcca ccagcttcct ccagaaactc tcagacctgg tgggcaggga cacccgactc
     3721 agcaacctgc tgagggagca gtggccagag aggcggcgat gccacaggtt ggagaatggc
     3781 tgtgggaatg catctcctca gatgaaggcc cggaacttgc ctgtttggct ccccaaccag
     3841 gacgagcccc ttcctggcag cagctgtgcc atccaagttg gggataaagt cccctatgac
     3901 atctgccggc cagaccactc agtgttgacc ctgcagctgc ctgtgacagc ctccgtgaga
     3961 gaggtgatgg cagcgttggc ccaggaggat ggctggacca aggggcaggt gctggtgaag
     4021 gtcaattctg caggtgatgc cattggcctg cagccagatg cccgtggtgt ggccacatct
     4081 ctggggctca atgagcgtct ctttgttgtc aacccacagg aagcgcatga gctgatccca
     4141 caccctgacc agctggggcc cactgtgggc tctgctgagg ggctggacct ggtgagtgcc
     4201 aaggacctgg caggccagct gacggaccac gactggagcc tcttcaacag tatccaccag
     4261 gtggagctga tccactatgt gctgggcccc cagcatctgc gggatgtcac caccgccaac
     4321 ctggagcgct tcatgcgccg cttcaatgag ctgcagtact gggtggccac cgagctgtgt
     4381 ctctgccccg tgcccggccc ccgggcccag ctgctcagga agttcattaa gctggcggcc
     4441 cacctcaagg agcagaagaa tctcaattcc ttctttgccg tcatgtttgg cctcagcaac
     4501 tcggccatca gccacctagc ccacacctgg gagcggctgc cccacaaagt ccggaagctg
     4561 tactccgccc tcgagaggct gctggacccc tcatggaacc accgggtata ccgactggcc
     4621 ctcgccaagc tctcccctcc tgtcatcccc ttcatgcccc ttcttctcaa agacatgacc
     4681 ttcattcatg agggaaacca cacactagtg gagaatctca tcaactttga gaagatgaga
     4741 atgatggcca gagtcgcgcg gatgctgcac cactgccgaa gccacaaccc tgtgcctctc
     4801 tcaccactca gaagccgagt ttcccacctc cacgaggaca gccaggtggc gaggatttcc
     4861 acatgctcgg agcagtccct gagcacccgg agtccagcca gcacctgggc ttatgtccag
     4921 cagctgaagg tcattgacaa ccagcgggaa ctctcccgcc tctcccgaga gctggagcca
     4981 gagctcatgc ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac
     5041 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5101 atggacgagc tgtacaaggg aggtaccggt ggatctatgg tgagcaaggg cgaggagctg
     5161 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5221 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
     5281 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
     5341 cttcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5401 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5461 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5521 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5581 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     5641 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     5701 atcggcgacg gccccgtgct gtaagaattc tgcagatatc catcacactg gcggccgctc
     5761 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     5821 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5881 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5941 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6001 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6061 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6121 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6181 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6241 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6301 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6361 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6421 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6481 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6541 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6601 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6661 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6721 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6781 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6841 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6901 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6961 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7021 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     7081 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     7141 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     7201 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     7261 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     7321 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     7381 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     7441 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     7501 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     7561 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     7621 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7681 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     7741 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7801 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7861 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7921 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7981 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8041 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8101 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8161 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8281 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8881 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9541 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9841 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10081 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10141 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10201 gcacatttcc ccgaaaagtg ccacctgacg tc
//
